Multiparametric Characterization of Telomere Length in T Cells by Flow-FISH [0.03%]
流式细胞仪结合荧光原位杂交技术多参数分析T细胞端粒长度
Macedonia Trigueros,Yacine Kharraz,Hammad Alzayat et al.
Macedonia Trigueros et al.
Telomere shortening is a hallmark of aging and immune dysfunction, offering a reliable indicator of cellular replicative history and biological age. Measuring telomere length in defined immune subsets provides critical insights into immune ...
Current Overview and Main Challenges of the Use of Galleria mellonella in Biomedical Research [0.03%]
"Galleria mellonella在生物医学研究中的应用现状及主要挑战"
Fabiana Alves de Souza Silva,Maria Eduarda da Silva Costa,Patrícia Michelle Nagai de Lima et al.
Fabiana Alves de Souza Silva et al.
Larvae of the greater wax moth Galleria mellonella have become an increasingly important in vivo model for biomedical research, providing a practical, ethical, and biologically relevant alternative to vertebrate organisms. Its suitability a...
Cellular Characterization Using Microwave-Assisted Immunocytochemistry, A Time-Efficient Alternative to Traditional Immunocytochemistry [0.03%]
基于微波免疫细胞化学的高效细胞表征新技术
Aidyn M Medina-López,Irma I Torres-Vázquez,Noraida Martínez-Rivera et al.
Aidyn M Medina-López et al.
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are related but separate techniques used for cell and tissue characterization. ICC involves immunolabeling of cells, and IHC immunolabeling of tissues; thus, the main difference is sa...
Absolute Quantification of microRNA Copies in Exosomes Using Real-Time PCR [0.03%]
使用实时PCR法对外泌体中微小核糖核酸进行绝对定量的方法
Pragati Raghuwanshi,Prasenjit Srivastava,Nishant Singh et al.
Pragati Raghuwanshi et al.
Exosomes are small extracellular vesicles (50 to 150 nm in size) that carry different kind of biomolecules in their lumen and facilitate intercellular communication. Serum contains a large number of exosomes (∼1012/ml of serum) that contai...
Co-Culture Systems to Study Epithelial-Immune Interactions During SARS-CoV-2 Infection [0.03%]
用于研究SARS-CoV-2感染期间上皮细胞-免疫细胞相互作用的共培养系统
Scott H Randell,Katherine C Barnett
Scott H Randell
Robust inflammatory responses to viral infection are mediated by immune cell populations, including monocytes and dendritic cells. However, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) does not replicate efficiently in these...
Acid-Catalyzed Cycloaddition of Guanines with TMOP: A Unified Strategy for Synthesizing Tricyclic Acyclovir Analogues and M1G-Containing Oligonucleotides [0.03%]
酸催化鸟嘌呤与TMOP的环加成反应：合成三环阿昔洛韦类似物和含M1G的寡核苷酸的统一策略
Zhuo-Yue Jia,Bin Wu,Wen-Wu Sun
Zhuo-Yue Jia
This article describes an efficient acid-catalyzed cyclization strategy for constructing the tricyclic pyrimido[1,2-a]purin-10(3H)-one scaffold via the reaction of guanine derivatives with 1,1,3,3-tetramethoxypropane (TMOP) catalyzed by 2,4...
A Hierarchical Screening Strategy for Genome-Edited Events in Polyploid Species: A Case Study on Hexaploid Common Wheat [0.03%]
六倍体普通小麦基因组编辑事件的层次筛选策略研究
Mingjiu Li,Guofeng Gao,Yuling Jiao
Mingjiu Li
Genome editing via CRISPR/Cas9 has been widely adopted in cereal crops. In diploid species such as rice and barley, the generation of knockout mutants is relatively straightforward for functional characterization of the genes of interest du...
A Tutorial for Isolating, Characterizing, and Inducing Presenescence in Human Periodontal Ligament and Dental Pulp Stem Cells [0.03%]
牙周膜和牙髓干细胞衰老诱导、鉴定及生物学特性分析的实验方法学研究
Kamila Sauer Veiga Leme,Márjorie de Assis Golim,Aline Márcia Marques Braz et al.
Kamila Sauer Veiga Leme et al.
For several years, scientists have focused on studying mesenchymal stem cells because of their ability for self-regeneration, their potential for differentiation into multiple lineages (e.g., osteogenic, chondrogenic, and adipogenic cells),...
Synthesis of 2',4'- and 3',4'-Bridged Nucleosides via 4'-Carbon Radicals Generated by H-Abstraction [0.03%]
通过H- abstraction生成的4'-碳自由基合成2',4'-和3',4'-桥连核苷
Yuta Ito,Yoshiyuki Hari
Yuta Ito
This unit describes a method for the synthesis of 2',4'- and 3',4'-bridged nucleosides from nucleosides bearing oxime imidate moieties at the 5' position. Hydrogen abstraction at the 4'-position of nucleosides by iminyl radicals derived fro...
Synthesis of Oligonucleotide RNA Containing 2´-Deoxy-2´-Fluoroarabinoguanosine (2´F-araG) for Stabilization of the Human Telomeric RNA G-Quadruplex [0.03%]
合成含有2'脱氧-2'-氟阿拉伯果糖腺苷(2'F-araG)的人类端粒RNA-G四联体稳定的寡核苷酸RNA
Hisao Saneyoshi,Rie Iwakiri,Shiyu Wang et al.
Hisao Saneyoshi et al.
This article provides detailed synthetic protocols for the preparation of RNA oligonucleotides containing 2'-deoxy-2'-fluoroarabinoguanosine (2'F-araG). Incorporation of this modification into a human telomeric RNA sequence stabilizes the G...