Assessment of Doxorubicin Internalization and Cytotoxicity in Primary Tumor Spheroids After Collagen Digestion [0.03%]
消化胶原蛋白后评估多柔比星在原发肿瘤类器官球体中的内吞作用及细胞毒效应
Alessandra Lo Cicero,Gabriele Lo Buglio,Simona Campora et al.
Alessandra Lo Cicero et al.
The limited penetration of chemotherapeutic agents into solid tumors remains a major obstacle to effective cancer treatment and is strongly influenced by the extracellular matrix (ECM). Three-dimensional (3D) tumor spheroids derived from pr...
Ta&#x;Aliyah M Jones,Stephen Hurst,Michael J Bround
Ta&#x;Aliyah M Jones
Mitochondrial calcium (mCa2+) homeostasis promotes oxidative metabolism within the physiological range; however, dysregulation can trigger necrotic cell death in diseases such as cardiac ischemia-reperfusion injury, muscular dystrophy, and ...
Liquid-Phase Synthesis of DNA Aptamer-Based PROTACs Using the Postsynthesis Conjugation Method [0.03%]
基于 postsynthesis 偶联法的 DNA 适配体基液相PROTAC合成法
Hitomi Terauchi,Genichiro Tsuji,Yosuke Demizu
Hitomi Terauchi
Proteolysis-targeting chimeras (PROTACs) have emerged as a promising strategy for the selective degradation of intracellular proteins by harnessing the ubiquitin-proteasome system. However, the development of PROTACs is often limited by the...
Sequence-Specific Primer Polymerase Chain Reaction Genotyping of Interleukin-22 Single Nucleotide Polymorphisms rs2227473 and rs2227513 with Validation by Sanger Sequencing [0.03%]
基于Sanger测序验证的白细胞介素-22基因单核苷酸多态性位点rs2227473和rs2227513的序列特异性引物聚合酶链锁反应基因分型研究
Julyane Schavaren,Matheus Braga,Aléia Harumi Uchibaba Yamanaka et al.
Julyane Schavaren et al.
Single nucleotide polymorphisms (SNPs) can modify protein expression or function, influencing susceptibility to various diseases. Interleukin-22 (IL-22), involved in epithelial barrier homeostasis and tissue repair, has shown complex roles ...
Sequence-Specific Primer Polymerase Chain Reaction Genotyping of the Human TLR9 Promoter Polymorphism rs187084 (-1486T>C) [0.03%]
人类TLR9启动子多态位点rs187084(-1486T>C)的序列特异性引物PCR基因分型法
Guilherme Castellani,Fernanda Pelisson Massi,Aléia Harumi Uchibaba Yamanaka et al.
Guilherme Castellani et al.
This article describes a validated and optimized sequence-specific primer polymerase chain reaction (PCR-SSP) protocol for genotyping the human TLR9 promoter polymorphism rs187084 (-1486T>C). Promoter single nucleotide polymorphisms (SNPs) ...
Identifying Donor Immune Cells in the Intestinal Mucosa of Chimeric Recipient Mice by Immunofluorescence [0.03%]
免疫荧光鉴定供者免疫细胞在嵌合受体小鼠肠道黏膜中的分布
Vinicius Canale,Samuel Asiedu,Fuad Malki et al.
Vinicius Canale et al.
Murine competitive congenic bone marrow transplantation is an important method used to identify genetic contributions to immune cell abundance, activation, differentiation, and mobilization, either in the bone marrow or in the peripheral or...
Automated Optimization of Bacterial Tracking Pipelines With TrackMate 8 [0.03%]
利用TrackMate 8自动化细菌追踪管道的优化
Marie Anselmet,Laura Xénard,Marvin Albert et al.
Marie Anselmet et al.
Quantitative analysis of bacterial dynamics in time-lapse microscopy requires robust tracking pipelines, yet selecting and optimizing algorithms for specific experiments remains challenging. Indeed, microbiologists are confronted with numer...
Quantification of Appetitive and Mechanical Aversive Associative Learning Paradigms in Drosophila via a Y-maze Assay [0.03%]
利用Y迷宫测定果蝇食欲和机械厌恶关联学习量化的定量分析
Samuel J Mabry,Thaanvi Malgireddy,Sean Sarkissian et al.
Samuel J Mabry et al.
Learning and memory are essential for the acquisition, storage, and recall of information, enabling organisms to adapt to changes in their environment. Associative learning begins when a stimulus, either positive or negative in valence, is ...
Quantitative Imaging of RNA in Plants Using Hybridization Chain Reaction-RNA Fluorescence in Situ Hybridization (HCR RNA-FISH) [0.03%]
基于杂交链反应的RNA荧光原位杂交技术用于植物中RNA的成像及定量分析
Sessen Daniel Iohannes,Nandhakumar Shanmugaraj,David Jackson
Sessen Daniel Iohannes
Quantitative RNA imaging in large plant tissues has historically been challenging because of limited spatial resolution, low signal-to-noise ratios, and the largely qualitative nature of traditional RNA in situ hybridization methods. Hybrid...
Engineering, Expression, Purification, and Application of Glycosaminoglycan-Specific Antibodies [0.03%]
岩藻糖甘露聚糖特异性抗体的工程改造、表达、纯化及应用
Kheerthana Duraivelan,Sriram Sundaravel,Esther N Njoroge et al.
Kheerthana Duraivelan et al.
Cluster of differentiation markers have been transformative for defining cell populations and their functional states, but recent work indicates that finer granularity can be achieved by considering the diverse heparan sulfate structures pr...