Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung [0.03%]
成人人类肺泡上皮细胞的永生化以研究远端肺部环境暴露
Evelyn Tran,Laurence St Pierre,Diego Velarde et al.
Evelyn Tran et al.
Alveolar epithelial cell type 1 (AT1) and type 2 (AT2) cells make up the saccular gas exchange units of the lung, called alveoli. Formation of alveoli during lung development accounts for the expansive surface area of the lung, allowing for...
Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand [0.03%]
表面等离子体共振（SPR）工作流程比较分析纳米抗体变异体与模型配基溶菌酶的结合能力
Escarlet Díaz-Galicia,Nicoleta Gutu,Yuli Peng et al.
Escarlet Díaz-Galicia et al.
Developing protein interaction-based technologies such as biosensors requires a clear understanding of receptor-target kinetics. Nanobodies, which are camelid-derived single-domain antibodies, are ideal biosensor receptors due to their high...
Point-of-CARE INFRARED IMAGING for Early Detection and Screening of Oral Cancer [0.03%]
口腔癌早期检测与筛查的临场关怀红外成像技术
Shalini R Gupta,Ravibabu Mulaveesala,Mani Kalaivani
Shalini R Gupta
India accounts for one-third of the global incidence and mortality of oral cancer. The national oral cancer screening program uses Conventional Oral Examination by Primary Healthcare (PHC) workers. This subjective assessment leads to unnece...
Cell Painting PLUS: An Iterative Staining-Elution Protocol for High-Content Phenotypic Screenings [0.03%]
细胞染色PLUS：用于高内涵表型筛选的迭代染色-洗脱协议
Marlene Wedler,Elena von Coburg,Jose M Muino et al.
Marlene Wedler et al.
Cell Painting (CP) methods use a combination of fluorescent dyes to label multiple cellular compartments simultaneously, enabling the comprehensive analysis of phenotypic changes through morphological profiling. Here, we present a detailed ...
A Standardized Unpredictable Chronic Stress Protocol for Adult Zebrafish [0.03%]
适用于成年斑马鱼的标准化不可预测慢性压力方案
Matheus Gallas-Lopes,Ana P Herrmann,Angelo Piato
Matheus Gallas-Lopes
The unpredictable chronic stress (UCS) protocol, originating in the 1980s, has contributed to depression research by inducing behavioral and physiological changes in rodents that resemble the symptoms observed in patients. Its translational...
Cholesterol-Induced Senescent Macrophages and Evaluation of Cellular Senescence Phenotypes [0.03%]
胆固醇诱导的衰老巨噬细胞及细胞衰老表型评估
Ivan A Salladay-Perez,Anthony J Covarrubias
Ivan A Salladay-Perez
Recent discoveries linking cellular senescence to inflammaging and age-associated metabolic diseases have intensified interest in defining the mechanisms that drive cellular senescence. Single-cell transcriptomic studies have identified tis...
Determination of Total Soluble Sugars in Pteridophytes Using the Anthrone Method [0.03%]
蒽酮法测定pteridophyta中的总可溶性糖类
Pablo Denova-Lozano,Alejandra Chamorro-Flores,Amparo B Cerón-Carpio et al.
Pablo Denova-Lozano et al.
Sugars serve as crucial integrators of both internal and environmental signals in plants, shaping the regulation of diverse physiological processes that occur throughout the plant's life span, from early embryogenesis to later senescence st...
Michelle C Krzyzanowski,Helen Pan,Iris Glaze et al.
Michelle C Krzyzanowski et al.
Because of the urgent need to better understand the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission mechanism and clinical outcomes resulting from infection, health organizations and research institutes developed d...
Grasiela Torres,Utkarsh Tripathi,Ivan Salladay-Perez et al.
Grasiela Torres et al.
We present a reproducible in vitro protocol for harvesting and culturing murine bone marrow-derived macrophages, with the added capability of freezing bone marrow cells at -80°C for scalability and long-term storage. To induce macrophage s...
Laboratory Protocols for Candidatus Liberibacter asiaticus Transmission Biology and Management Research [0.03%]
亚洲 Liberibacter asiaticus 传输生物学和管理研究的实验室方案
Diogo M Galdeano,William Ingram,Anna Erickson et al.
Diogo M Galdeano et al.
Candidatus Liberibacter asiaticus (CLas), the causal agent of Huanglongbing, is transmitted by the Asian citrus psyllid (Diaphorina citri) in a persistent, propagative manner. Reproducible transmission studies require not only controlled in...