Standardization of Immunohistochemistry Using an Anti-WASLB Antibody in the Skeletal Muscle of Teleost Fish [0.03%]
利用辐鳍鱼骨骼肌中的抗WASLB抗体进行免疫组化的标准化研究
Erika Stefani Perez,Matheus Naia Fioretto,Bruna Tereza Thomazini Zanella et al.
Erika Stefani Perez et al.
Immunohistochemistry (IHC) is a widely used technique for the identification, localization, and analysis of protein expression in tissues, playing a critical role in pathological diagnosis and validation of data derived from molecular biolo...
Zebrafish Embryos to Profile Nano(bio)materials: A Modular Platform for Developmental Toxicity, Neurotoxicity, and Inflammation-Regeneration Assays [0.03%]
斑马鱼胚胎用于纳米（生物）材料特征分析：一个模块化平台，用于发育毒性、神经毒性和炎症再生试验
Cinzia Bragato,Alessandra Lama,Greta Carbone Faccin et al.
Cinzia Bragato et al.
Zebrafish embryos provide a rapid, low-cost in vivo platform to profile the developmental, neurotoxic, and immunomodulatory effects of engineered nano(bio)materials within a single vertebrate system. This article presents a modular workflow...
William J Jones,Rhea Couper,Gwyn W Gould et al.
William J Jones et al.
Obesity and type 2 diabetes (T2D) have reached epidemic proportions worldwide, affecting millions of people and contributing to serious health complications, such as metabolic syndrome, cardiovascular disease, and cancer. Adipose tissue is ...
Enhancing Biological Activity in Skin Repair with a Transdermal Peptide-Recombinant Type III Collagen Hydrogel [0.03%]
一种经皮肽-重组III型胶原水凝胶在皮肤修复中的促活性研究
Yue Liu,Jingjun Hong
Yue Liu
Skin wound healing is a complex biological process requiring coordinated cellular responses and extracellular matrix remodeling. Type III collagen (COL3A) plays a critical role in early-stage tissue repair, but its direct application is lim...
High-Content Immunofluorescence Quantification of Transcription Factors: Application to pSMADs for BMP/TGFβ Signaling Pathways [0.03%]
高内涵免疫荧光定量测定转录因子：在骨形态发生蛋白/转化生长因子β信号途径中pSMADs的应用
Valia Khodr,Paul Machillot,Catherine Picart
Valia Khodr
High-throughput immunofluorescence (IF) detection has become an essential tool in drug discovery for analyzing multiple markers and providing insights into cellular pathways. While IF techniques have been established for decades, high-conte...
MccB-catalyzed C-terminal Thioesterification for Protein Bioconjugation [0.03%]
McCB催化的蛋白质C末端硫酯化反应及其生物偶联应用
Di Yang,Amy M Weeks
Di Yang
Protein bioconjugation enables the installation of functional groups, probes, and payloads that cannot be genetically encoded, thereby enabling the study and manipulation of biological systems. An important class of intermediates in protein...
Deploying a JupyterHub Server for Academic Research Using Netbooks as an Example [0.03%]
使用Netbook为例部署JupyterHub服务器以供学术研究使用
Marouen Ben Guebila,Enakshi Saha,Mia Shapoval et al.
Marouen Ben Guebila et al.
Jupyter Notebooks have transformed the way we conduct computational research thanks to a versatile platform that combines code, annotations, plots, and a lightweight format that enables versioning. However, steps for installing dependencies...
Induction of Murine Models of Autoimmune Myocarditis, Valvulitis, and Cardiomyopathy [0.03%]
自身免疫性心肌炎、瓣膜炎和心肌病的小鼠模型的诱导
Jennifer M Myers,DeLisa Fairweather,Sally A Huber et al.
Jennifer M Myers et al.
Myocarditis and valvulitis are inflammatory diseases affecting myocardium and heart valves. Myocarditis, a viral-induced disease of myocardium, may lead to dilated cardiomyopathy and loss of heart function, and valvulitis leads to deformed ...
Enzymatic Synthesis of NAD [0.03%]
酶法合成NAD+
Emily Boyle,Olivia Zhang,Yana Cen
Emily Boyle
Nicotinamide adenine dinucleotide (NAD+) is an essential metabolite involved in numerous biological processes, functioning both as a redox cofactor in cellular energy metabolism and as a co-substrate for NAD+-utilizing enzymes, including si...
Optimized MTT Assay for Metabolic Activity Evaluation in Human Spermatozoa [0.03%]
人精子代谢活性评价的优化MTT实验方法
Bárbara Guerra-Carvalho,Eva V Gonçalves,Mário Sousa et al.
Bárbara Guerra-Carvalho et al.
The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is low-cost, widely available, and mainly used to assess cell viability as a function of cellular oxidoreductase enzyme-dependent conversion of the water-soluble t...