Automated Optimization of Bacterial Tracking Pipelines With TrackMate 8 [0.03%]
利用TrackMate 8自动化细菌追踪管道的优化
Marie Anselmet,Laura Xénard,Marvin Albert et al.
Marie Anselmet et al.
Quantitative analysis of bacterial dynamics in time-lapse microscopy requires robust tracking pipelines, yet selecting and optimizing algorithms for specific experiments remains challenging. Indeed, microbiologists are confronted with numer...
Quantification of Appetitive and Mechanical Aversive Associative Learning Paradigms in Drosophila via a Y-maze Assay [0.03%]
利用Y迷宫测定果蝇食欲和机械厌恶关联学习量化的定量分析
Samuel J Mabry,Thaanvi Malgireddy,Sean Sarkissian et al.
Samuel J Mabry et al.
Learning and memory are essential for the acquisition, storage, and recall of information, enabling organisms to adapt to changes in their environment. Associative learning begins when a stimulus, either positive or negative in valence, is ...
Quantitative Imaging of RNA in Plants Using Hybridization Chain Reaction-RNA Fluorescence in Situ Hybridization (HCR RNA-FISH) [0.03%]
基于杂交链反应的RNA荧光原位杂交技术用于植物中RNA的成像及定量分析
Sessen Daniel Iohannes,Nandhakumar Shanmugaraj,David Jackson
Sessen Daniel Iohannes
Quantitative RNA imaging in large plant tissues has historically been challenging because of limited spatial resolution, low signal-to-noise ratios, and the largely qualitative nature of traditional RNA in situ hybridization methods. Hybrid...
Engineering, Expression, Purification, and Application of Glycosaminoglycan-Specific Antibodies [0.03%]
岩藻糖甘露聚糖特异性抗体的工程改造、表达、纯化及应用
Kheerthana Duraivelan,Sriram Sundaravel,Esther N Njoroge et al.
Kheerthana Duraivelan et al.
Cluster of differentiation markers have been transformative for defining cell populations and their functional states, but recent work indicates that finer granularity can be achieved by considering the diverse heparan sulfate structures pr...
Expression and Membrane Targeting of β1-integrin-ECFP in Mammalian Cells [0.03%]
哺乳动物细胞中的β1-整合素-ECFP的表达及其膜定位
Andres Martin Toscani,Federico Coluccio Leskow
Andres Martin Toscani
β1-integrin is a transmembrane glycoprotein that heterodimerizes with multiple α subunits and functions as a key receptor for extracellular matrix proteins. It contains a large extracellular ligand-binding domain, a single-pass transmembr...
A Facile Strategy for 3'-Terminal Functionalization of DNA and RNA via On-Column Conjugation with Integration into Tandem Oligonucleotide Synthesis [0.03%]
一种用于DNA和RNA 3'-末端官能化的简便策略及其在串联寡核苷酸合成中的应用
Jagandeep S Saraya,Derek K O&#x;Flaherty
Jagandeep S Saraya
Amino-functionalized oligonucleotides are valuable tools in therapeutics, biosensing, and nanotechnology. Although 5'-terminal modifications are readily accessible, incorporation at the 3'-terminus remains more challenging and often require...
Evaluation of Antibacterial Effects of Sargassum binderi-based Mouthwash via API 20E Profiling: Study Protocol for a Feasibility Randomized Controlled Trial [0.03%]
基于扁芽丝藻漱口水抗菌效果的API 20E特征研究：可行性随机对照试验方案
Ruslim Andre Kusuma,Nisrina Ekayani Nasrun,Muhammad Ruslin et al.
Ruslim Andre Kusuma et al.
The antibacterial effects of Sargassum binderi (brown macroalgae rich in phlorotannins and fucoidan) have not yet been explored in clinical settings, particularly regarding its use as an oral antimicrobial agent. This study aims to evaluate...
Brain Magnetic Resonance Elastography Experiments With an Electromagnetic Actuator [0.03%]
基于电磁激励的脑弹性图实验研究
Suhao Qiu,Yuan Feng,Guang-Zhong Yang
Suhao Qiu
Magnetic resonance elastography (MRE) enables non-invasive assessment of the mechanical properties of brain tissue, which is crucial for diagnosing neurodegenerative diseases and understanding brain development. This technique relies on ind...
Efficient Adaptation of Structure-Aware Protein Language Models for Diverse Protein Applications [0.03%]
高效适应结构感知的蛋白质语言模型以应用于各种蛋白质相关任务
Duolin Wang,Yichuan Zhang,Yongfang Qin et al.
Duolin Wang et al.
Protein language models (PLMs) trained solely on sequence data have significantly advanced our understanding of protein biology and achieved remarkable performance in protein prediction tasks. However, their lack of three-dimensional (3D) s...
Anzu Minami,Minami Shimizu,Shun Tamaki et al.
Anzu Minami et al.
Genome editing can enhance basic research and enable industrial applications of green algae. Here, we present an affordable, broadly applicable workflow for genome editing in the unicellular green alga Euglena gracilis using Cas9 nucleases....