High Throughput In vivo Imaging; A Pipeline for Automated Analysis of Longitudinal Preclinical Studies [0.03%]
高通量体内成像；纵向前期研究自动化分析流程
Mark Tchinov,Zachary Gurberg,Diana Möckel et al.
Mark Tchinov et al.
Fast acquisition times, multi-animal beds, and improved accessibility of preclinical imaging systems have enabled high-throughput imaging, significantly accelerating in vivo experimentation. However, image analysis remains largely manual, l...
Decoding Viral Dark Matter: Metagenomic Prokaryotic Virus Characterization With Pharokka, Phold, and Phynteny [0.03%]
破解病毒暗物质：用Pharokka、Phold和Phynteny表征宏基因组原核病毒
George Bouras,Susanna R Grigson,Lindsay Durr et al.
George Bouras et al.
Viral metagenomics is an increasingly powerful tool for understanding the function and structure of viruses across the diverse environments of our planet. However, decoding the functional potential of prokaryotic viral metagenomes is extrem...
A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers [0.03%]
评价根管充填密封材料微渗漏的新实验室方法学研究
Emannuel Fonseca Thomé da Silva Monteiro,Alice Abib Ramos Fabri,Marcelo Barbosa Garcia et al.
Emannuel Fonseca Thomé da Silva Monteiro et al.
To avoid failure of endodontic treatment, it is important to properly seal the root canals after chemomechanical preparation in order to avoid microleakage. Typically, sealing involves the use of gutta-percha and sealing cement, the latter ...
TRIAGE Toolkit: Streamlined Discovery of Regulatory Genes and Elements [0.03%]
TRIAGE工具箱：调控基因和元件的精简发现方法
Qiongyi Zhao,Sophie Shen,Yuliangzi Sun et al.
Qiongyi Zhao et al.
Efficient discovery of regulatory genes and elements is essential for understanding cell identity, differentiation, and disease mechanisms. The TRIAGE methods are a set of well-established computational approaches that identify context-spec...
High-throughput Profiling of Pseudouridines in Microbiome-derived Bacterial RNA [0.03%]
微生物组细菌RNA中的假尿苷高通量分析法
Shikha Sharma,Ning Duan,Akintunde Emiola
Shikha Sharma
Pseudouridine (Ψ) is a widespread RNA modification that influences RNA stability, structure, and translation. However, its role in bacterial mRNA, particularly within complex microbiomes, remains poorly defined. Here, we describe a bisulfi...
Michael Akintubosun,Melanie A Higgins
Michael Akintubosun
Producing recombinant bacterial proteins is essential for elucidating their structure and function, making efficient overexpression systems a key requirement. Escherichia coli is widely used for this purpose, particularly for bacterial prot...
Streamlined In Vitro Transcription for Generating Self-Amplifying RNA With Modified Nucleotides [0.03%]
一种用于生成带修饰核苷酸的自我增殖型RNA的简化体外转录方法
Harish Prakash,Karthik Kadhir Velu,Vasanth Thamodaran
Harish Prakash
Self-amplifying RNA (saRNA) derived from alphavirus replicons enables robust intracellular RNA amplification and high-level protein expression at substantially lower doses than nonreplicating messenger RNA (mRNA) platforms. Venezuelan equin...
James Phie,Joshua N Johnstone,Cameron Fraser et al.
James Phie et al.
Concatenating Original Duplex for Error Correction (CODEC) is a duplex sequencing library preparation method that physically links the Watson and Crick strands of individual DNA molecules. Despite growing interest in cost-effective duplex s...
Standardized Pipeline for Metabolism and Cognition in GO-DS21 Mouse Model: Investigating Down Syndrome Comorbidities [0.03%]
GO-DS21小鼠模型中代谢和认知的标准化流程：探究唐氏综合症共病问题
Mohammed Selloum,Patricia da Silva-Buttkus,Fabrice Riet et al.
Mohammed Selloum et al.
Down syndrome (DS) is the most prevalent form of intellectual disability (ID) globally, with an incidence rate of approximately 1 in 1000 births, affecting over 5 million individuals worldwide. DS is characterized by a genetic profile that ...
Effect of Passive Hamstring Stretching on Craniovertebral Angle, Muscle Activation Pattern and Proprioception in Individuals with Forward Head Posture: Study Protocol for Randomized Controlled Trial [0.03%]
被动腘绳肌牵拉对前倾头部姿势个体颅椎角、肌肉激活模式和本体感觉影响的随机对照试验研究方案：一项随机对照试验的研究方案
Musawir Mohsin Parsa,Zubia Veqar,Shabnam Khan et al.
Musawir Mohsin Parsa et al.
Background: Forward head posture (FHP) is a common musculoskeletal condition associated with altered craniovertebral angle (CVA), reduced cervical range of motion (CROM), and impaired muscle activation. Conventional treat...