High-throughput Method for Novel Medium Development for Culture of Anaerobic Gut Bacteria [0.03%]
一种用于厌氧肠道细菌培养的新培养基开发的高通量方法
Kristina Wornell,Bikiran Pardesi,Kevin Lee et al.
Kristina Wornell et al.
Gut microbiota play important roles in the health of their host and detailed investigation of these organisms requires in vitro culture. Culturing strictly anaerobic bacteria can be a challenge as the gut environment they inhabit is nutriti...
Methods to Identify Immune Cells in Tissues With a Focus on Skin as a Model [0.03%]
以皮肤为例识别组织中免疫细胞的方法
Peter R Murphy,Divyaa Narayanan,Snehlata Kumari
Peter R Murphy
The skin protects our body from external challenges, insults, and pathogens and consists of two layers, epidermis and dermis. The immune cells of the skin are an integral part of protecting the body and essential for mediating skin immune h...
Receptor Interactome Discovery with RDIMIS, a Membrane Protein Interaction Screen Using Recombinant Extracellular Vesicles [0.03%]
利用重组外泌体的膜蛋白相互作用筛选（RDIMIS）发现受体互作组
Ariana D Sanchez,Sean M Peterson,Mike Reichelt et al.
Ariana D Sanchez et al.
Membrane protein interactions are challenging to identify because of the unique biophysical characteristics of both transmembrane proteins and membrane environments. The Receptor Display in Membranes Interaction Screen (RDIMIS) platform ove...
Laser Capture Microdissection Optimization for High-Quality RNA in Mouse Brain Tissue [0.03%]
小鼠脑组织激光捕获微解剖技术的优化以获得高质量RNA
Margareth Nogueira,Daiane C F Golbert,Bruna Landeira et al.
Margareth Nogueira et al.
Laser Capture Microdissection (LCM) is a method that allows one to select and dissect well-defined structures, specific cell subpopulations, or even single cells from different types of tissue for subsequent extraction of DNA, RNA, or prote...
Using Full-Spectrum Flow Cytometry to Phenotype Memory T and NKT Cell Subsets with Optimized Tissue-Specific Preparation Protocols [0.03%]
采用全光谱流式细胞仪和组织特异性优化制备方案表征记忆T及NKT细胞亚群
Kathryn Farrand,Lauren E Holz,Laura Ferrer-Font et al.
Kathryn Farrand et al.
Full-spectrum flow cytometry is now routinely used in many laboratories internationally, and the demand for this technology is rapidly increasing. With capacity to use larger and more complex staining panels, standardized protocols are requ...
Isolating Nuclei From Frozen Human Heart Tissue for Single-Nucleus RNA Sequencing [0.03%]
从冷冻的人类心脏组织中分离单细胞核进行单细胞核RNA测序
Sina Safabakhsh,Funda Sar,Luciano Martelotto et al.
Sina Safabakhsh et al.
Heart disease is the leading cause of global morbidity and mortality. This is in part because, despite an abundance of animal and in vitro models, it has been a challenge to date to study human heart tissue with sufficient depth and resolut...
Akiharu Kubo,Satomi Aoki,Harumi Fujita
Akiharu Kubo
The epidermis is a stratified epithelium. Compared to that for monolayered epithelia, understanding of the cell biology of stratified epithelia lags far behind. The major reason for this is the limitation of methods to reproduce the epiderm...
High-Throughput Assessments for Storage Stability, In Vitro Release, and Particle Size of Encapsulated Biocontrol Fungi in Hydrogel Beads [0.03%]
用于水凝胶珠中包埋生物控制真菌的储存稳定性、体外释放和粒径的高通量评价方法研究
Adriana Santos-Díaz,Liz Uribe-Gutiérrez,Mauricio Cruz-Barrera
Adriana Santos-Díaz
Use of biocontrol fungi (BF) such as Trichoderma spp. minimizes fungicide input and increases both plant nutrition and protection from disease. Thus, the introduction of BF by novel inoculants in crop management is an excellent strategy to ...
Co-Culture of Human Primary Hepatocytes and Nonparenchymal Liver Cells in the Emulate® Liver-Chip for the Study of Drug-Induced Liver Injury [0.03%]
人原代肝细胞和非实质细胞在Emulate®肝脏芯片中的共培养用于研究药物引起的肝损伤
Qiang Shi,Ayesha Arefin,Lijun Ren et al.
Qiang Shi et al.
Drug-induced liver injury (DILI) is a significant public health issue, but standard animal tests and clinical trials sometimes fail to predict DILI due to species differences and the relatively low number of human subjects involved in preap...
Xinyun Su,Sangeeth Pillai,Younan Liu et al.
Xinyun Su et al.
Primary cells are an essential tool for in vitro studies and are obtained directly from living tissues or organs. They closely mimic the physiological state and maintain in vivo functions for short periods of time under optimal conditions. ...