Efficient Cas9-based Genome Editing Using CRISPR Analysis Webtools in Severe Early-onset-obesity Patient-derived iPSCs [0.03%]
利用CRISPR分析网络工具在严重早发性肥胖病人诱导多能干细胞中进行高效的Cas9基因组编辑技术
Achchhe Patel,Grazia Iannello,Alejandro Garcia Diaz et al.
Achchhe Patel et al.
The CRISPR system is an adaptive defense mechanism used by bacteria and archaea against viruses and plasmids. The discovery of the CRISPR-associated protein Cas9 and its RNA-guided cleavage mechanism marked the beginning of a new era in gen...
Case Reports
Current protocols. 2022 Aug;2(8):e519. DOI:10.1002/cpz1.519 2022
Frederik Helmprobst,Anna Hupfer,Matthias Lauth et al.
Frederik Helmprobst et al.
The composition of the cell culture environment profoundly affects cultured cells. Standard cell culture equipment such as plastic and glass provide extremely stiff surfaces compared to physiological cell environments (i.e., tissue). A grow...
In Vitro Mapping of Nucleosome Positions at Base-Pair Resolution Using Ortho-Phenanthroline [0.03%]
一种用于以碱基对分辨率进行体外核小体定位的试剂：邻菲啰啉
Samaneh Ghassabi Kondalaji,Gregory D Bowman
Samaneh Ghassabi Kondalaji
The positions of nucleosomes along genomic DNA play a role in defining patterns of gene expression and chromatin organization. Determination of nucleosome positions in vivo and in vitro, as revealed by the locations of histones on DNA, has ...
Study of Calcium Signaling in Astrocytes with a Novel Endoplasmic Reticulum-Targeted GCaMP Sensor [0.03%]
一种新型靶向内质网的GCaMP钙离子荧光传感器及其在星形胶质细胞钙信号转导研究中的应用
Surya P Aryal,Mengfan Xia,Pavel I Ortinski et al.
Surya P Aryal et al.
The endoplasmic reticulum (ER), the major organelle for the storage of Ca2+ , maintains a concentration of Ca2+ much higher than in the cytosol or other subcellular organelles, such as the mitochondria. A variety of tools have been develope...
The Use of Precision-Cut Lung Slices for Studying Innate Immunity to Viral Infections [0.03%]
精密制备的肺组织切片在研究病毒天然免疫中的应用
Christina Michalaki,Charlotte Dean,Cecilia Johansson
Christina Michalaki
Precision-cut lung slices (PCLS) are a novel tool to study cells of the lower airways. As PCLS retain the integrity and architecture of the lung, they constitute a robust model for studying the cells of the lower respiratory tract. Use of P...
Intravital Microscopy to Visualize Murine Small Intestinal Intraepithelial Lymphocyte Migration [0.03%]
活体成像技术可视化小鼠小肠上皮内淋巴细胞迁移现象
Matthew Fischer,Karen L Edelblum
Matthew Fischer
Intraepithelial lymphocytes (IELs) are critical sentinels involved in host defense and maintenance of the intestinal mucosal barrier. IELs expressing the γδ T-cell receptor provide continuous surveillance of the villous epithelium by migr...
High-Throughput Cell-Free Screening of Eukaryotic Membrane Proteins in Lipidic Mimetics [0.03%]
基于脂质类似物的真核生物膜蛋白无细胞高通量筛选
Renato Bruni
Renato Bruni
Membrane proteins (MPs) carry out important functions in the metabolism of cells, such as the detection of extracellular activities and the transport of small molecules across the plasma and organelle membranes. Expression of MPs for bioche...
Donald P Evenson
Donald P Evenson
The Sperm Chromatin Structure Assay (SCSA® ) is a federally registered protocol for simultaneous flow cytometric measures of sperm DNA integrity and chromatin structure. Fresh or frozen/thawed raw semen samples are diluted in buffer to a s...
Claire Regan,Jonathan Preall
Claire Regan
The single-cell revolution in the field of genomics is in full bloom, with clever new molecular biology tricks appearing regularly that allow researchers to explore new modalities or scale up their projects to millions of cells and beyond. ...
Methods to Evaluate the Potential Clinical Significance of Antibodies to Red Blood Cells [0.03%]
评估红细胞抗体潜在临床意义的方法
Kayluz Frias Boligan,Gurleen Sandhu,Donald R Branch
Kayluz Frias Boligan
Immune-mediated red blood cell (RBC) destruction due to antibodies is an ongoing problem in transfusion medicine for the selection of the safest blood. Serological testing often revealed incompatibility with donors' RBCs. When this incompat...