Fengjun Lv,Shuangting Lang,Ximing Qin
Fengjun Lv
Mice, as one of the most important model organisms in life science research, rely heavily on assisted reproductive technologies, particularly in vitro fertilization (IVF) and embryo transfer (ET), which serve as indispensable platforms for ...
Transduction of Quiescent Human Hematopoietic Stem and Progenitor Cells Using Lentiviral Vectors and Virus-Like Particles [0.03%]
采用慢病毒和类似病毒颗粒转导静息的人造血干细胞和祖细胞
Denise Klatt,Christian Brendel
Denise Klatt
Traditional retroviral gene transfer protocols for the genetic modification of hematopoietic stem and progenitor cells (HSPC) include a multiday ex vivo culture period, which can negatively affect the biology of the cells and is costly. As ...
Preparation of Human Lymphocyte Proteins to Profile Targets of Oxidative Stress [0.03%]
用于研究氧化应激靶点的人淋巴细胞蛋白质的制备
James A McCardle,David O Carpenter,David A Lawrence
James A McCardle
This article provides detailed methods for preparing membrane and cytosolic proteins isolated from human CD4+ T cells to identify biomarkers of oxidative stress (OS). Proteins important for T-cell metabolism, differentiation, and redox home...
Correction: Efficient Site-Directed Mutagenesis Mediated by Primer Pairs with 3'-Overhangs [0.03%]
关于高效定点突变的一封更正信：使用3'突出端引物对进行定点突变
Negar Mousavi,Ethan Zhou,Arezousadat Razavi et al.
Negar Mousavi et al.
Published Erratum
Current protocols. 2026 Jan;6(1):e70311. DOI:10.1002/cpz1.70311 2026
Evaluating Mineral Deposition in Bone-Regenerative Collagen-Based Scaffolds Using High-Resolution microCT and 3D Computational Segmentation [0.03%]
基于 collagen 的骨再生支架中矿化沉积的评价：高分辨率 microCT 和 3D 计算机断层摄影术的应用
Youngnam Kang,Kaavian Shariati,Catherine T Cascavita et al.
Youngnam Kang et al.
Bone regeneration depends on coordinated interactions among cells, extracellular matrices, and the dynamic microenvironment within engineered constructs. Mineralized collagen-glycosaminoglycan (MC-GAG) scaffolds are designed to replicate ke...
Rachel D McConnell,Crinan Jarrett,Diogo F Ferreira et al.
Rachel D McConnell et al.
Fecal DNA metabarcoding is a powerful tool for examining animal diets with unprecedented resolution, offering insights into ecological patterns shaped by trophic interactions. As a result, dietary metabarcoding has become widely applied acr...
Multi-Cellular Human Liver Organoids for Modeling Metabolic Dysfunction-Associated Steatotic Liver Disease and Drug-Induced Liver Injury [0.03%]
代谢功能异常相关肝脂肪变性疾病和药物性肝损伤的体外模型研究——多细胞类器官技术及其应用初探
Maela Duclos,Zeineb Marzougui,Mariam Saleh et al.
Maela Duclos et al.
In vitro modeling of human liver function is essential for assessing drug-induced toxicity, particularly in the context of metabolic dysfunction-associated steatotic liver disease (MASLD). However, standard liver organoid systems often lack...
Seamless and Highly Efficient Site-directed Mutagenesis for Protein, RNA, and Plasmid Engineering [0.03%]
高效便捷的蛋白质、RNA和质粒定点突变技术
Xiang-Jiao Yang
Xiang-Jiao Yang
Site-directed mutagenesis is indispensable for protein, RNA, and plasmid engineering. It is ideal to carry out such mutagenesis at an efficiency close to 100%, but many current methods fail to reach this goal and thus require extensive scre...
Analysis of Protein and Lipid Dynamics Using Confocal Fluorescence Recovery After Photobleaching [0.03%]
confocal荧光漂白恢复法分析蛋白质和脂质的动力学
Charles A Day,Lewis J Kraft,Minchul Kang et al.
Charles A Day et al.
Fluorescence recovery after photobleaching (FRAP) is a powerful, versatile, and widely accessible tool to monitor molecular dynamics in living cells that can be performed using modern confocal microscopes. Although the basic principles of F...
Sunna Nabeela,Neha Agrawal,Priya Uppuluri
Sunna Nabeela
Dermatophytes are keratinophilic fungi that cause superficial infections of the skin, hair, and nails. The emergence of recalcitrant infections caused by Trichophyton mentagrophytes complex and T. indotineae, particularly in diabetic patien...