Mass Cytometry Workflow to Achieve High-Dimensional Immunophenotyping in Resource-Limited or Decentralized Environments [0.03%]
高通量质谱流式细胞术实现有限资源或去中心化环境下的免疫表型分析
Natalie Smith,Michael Cohen,Lauren Tracey et al.
Natalie Smith et al.
Globally, regional and remote communities are burdened by both an increased prevalence and worse prognosis of many infectious and chronic diseases. However, largely owing to logistical challenges, these communities are under-represented in ...
Virtual Morris Water Task: Procedures and Protocols for the Assessment of Spatial Navigation and Memory [0.03%]
虚拟莫里斯水迷宫任务：空间导航和记忆评估的方法与规程
Conor Thornberry,Jose M Cimadevilla,Sean Commins
Conor Thornberry
The original Morris water maze has been coined the "gold standard" task for examining spatial navigation in animals. The general procedure of the maze involves a circular pool filled approximately halfway with water. An animal is then taske...
Light Microscopy-Based Organelle Quantification: A Comprehensive Protocol [0.03%]
基于光学显微镜的细胞器定量分析：全面协议
Suraj Thapliyal,Hari Kalpana Namdar,Ronald McMillan et al.
Suraj Thapliyal et al.
Cellular organelles are not just static structures; they are highly dynamic and directly linked to cellular functions. Changes in their morphology can be early indicators of diseases. Recent advancements in light microscopy techniques have ...
MinION Nanopore-Enabled Identification and Genomic Characterization of Pseudomonas syringae Complex Infecting Blueberry Using Symptomatic Plant Samples and Pathogen Pure Culture [0.03%]
基于MinION纳米孔测序的侵染蓝莓的假单胞杆菌鉴定及其基因组特征分析（基于病原纯培养和植株样品）
Rishi R Burlakoti,Sanjib Sapkota,Pragyan Burlakoti et al.
Rishi R Burlakoti et al.
Pseudomonas syringae complex (Psc) causes bacterial blight and canker on blueberry and other fruit crops. Reliable and cost-effective pathogen detection is essential for managing crop diseases. The Oxford Nanopore MinION has emerged as a po...
Marc Sorigue,Rebeca Jurado,Michael D Ward et al.
Marc Sorigue et al.
Acoustic imaging cytometry integrates high-throughput flow cytometry with high-resolution brightfield imaging, enabling simultaneous quantitative and morphological analysis of cells. This technology employs acoustic focusing to align cells ...
Mass Spectrometry-Based Integrated Multiomics Platforms for Medicinal Plant Analysis [0.03%]
基于质谱的整合型多组学平台在药用植物分析中的应用
Cemil Can Eylem,Sevilay Erdoğan Kablan,Tuba Reçber et al.
Cemil Can Eylem et al.
Plant-based multiomics approaches provide powerful tools for elucidating metabolic regulation, biochemical diversity, and functional responses to genetic and environmental variation. However, plant matrices pose unique analytical challenges...
Correction: Rapid, Scalable, and Cost-Effective Manufacturing of Uniform Non-Enveloped, Tag-Free Virus-Like Particles [0.03%]
关于统一的非包膜无标签病毒样颗粒快速、可扩展且低成本制造的更正通知
Xiaoyan Wang,Yanhao Gu,Hong Luo et al.
Xiaoyan Wang et al.
Published Erratum
Current protocols. 2026 Feb;6(2):e70343. DOI:10.1002/cpz1.70343 2026
Correction: Preparation of Vitrified Mammalian Cells for In Situ Cryo-Electron Tomography [0.03%]
关于制备用于原位冷冻电子断层扫描的玻璃化哺乳动物细胞的纠正意见
Eva Martin-Solana,Sonia Frendi,Jiying Ning et al.
Eva Martin-Solana et al.
Published Erratum
Current protocols. 2026 Feb;6(2):e70344. DOI:10.1002/cpz1.70344 2026
A Novel Cell-Based PCR Method for Accurate Characterization of African Trypanosomes [0.03%]
一种新型的基于细胞的PCR方法，用于非洲锥虫的准确表征
Emmanuel Oluwadare Balogun,Gideon Ibrahim Joseph,Edwige Flore Gouegni et al.
Emmanuel Oluwadare Balogun et al.
African trypanosomes exhibit significant genetic diversity but share similar morphological characteristics, making effective surveillance and diagnosis challenging with conventional microscopy and other detection methods. While polymerase c...
AMPSeek: A Workflow for Predicting Antimicrobial Peptide Activity, Three-Dimensional Structure, and Toxicity [0.03%]
AMPSeek：预测抗菌肽活性、三维结构和毒性的工作流程
Emily Zhang,Berke Ucar,Ali Salehi et al.
Emily Zhang et al.
With antimicrobial resistance emerging as a top global health concern, new approaches to prevent and treat infections are urgently needed. Antimicrobial peptides are recognized as a promising alternative to conventional antibiotics. Conside...