Streamlined In Vitro Transcription for Generating Self-Amplifying RNA With Modified Nucleotides [0.03%]
一种用于生成带修饰核苷酸的自我增殖型RNA的简化体外转录方法
Harish Prakash,Karthik Kadhir Velu,Vasanth Thamodaran
Harish Prakash
Self-amplifying RNA (saRNA) derived from alphavirus replicons enables robust intracellular RNA amplification and high-level protein expression at substantially lower doses than nonreplicating messenger RNA (mRNA) platforms. Venezuelan equin...
James Phie,Joshua N Johnstone,Cameron Fraser et al.
James Phie et al.
Concatenating Original Duplex for Error Correction (CODEC) is a duplex sequencing library preparation method that physically links the Watson and Crick strands of individual DNA molecules. Despite growing interest in cost-effective duplex s...
Standardized Pipeline for Metabolism and Cognition in GO-DS21 Mouse Model: Investigating Down Syndrome Comorbidities [0.03%]
GO-DS21小鼠模型中代谢和认知的标准化流程：探究唐氏综合症共病问题
Mohammed Selloum,Patricia da Silva-Buttkus,Fabrice Riet et al.
Mohammed Selloum et al.
Down syndrome (DS) is the most prevalent form of intellectual disability (ID) globally, with an incidence rate of approximately 1 in 1000 births, affecting over 5 million individuals worldwide. DS is characterized by a genetic profile that ...
Effect of Passive Hamstring Stretching on Craniovertebral Angle, Muscle Activation Pattern and Proprioception in Individuals with Forward Head Posture: Study Protocol for Randomized Controlled Trial [0.03%]
被动腘绳肌牵拉对前倾头部姿势个体颅椎角、肌肉激活模式和本体感觉影响的随机对照试验研究方案：一项随机对照试验的研究方案
Musawir Mohsin Parsa,Zubia Veqar,Shabnam Khan et al.
Musawir Mohsin Parsa et al.
Background: Forward head posture (FHP) is a common musculoskeletal condition associated with altered craniovertebral angle (CVA), reduced cervical range of motion (CROM), and impaired muscle activation. Conventional treat...
Standardization of Immunohistochemistry Using an Anti-WASLB Antibody in the Skeletal Muscle of Teleost Fish [0.03%]
利用辐鳍鱼骨骼肌中的抗WASLB抗体进行免疫组化的标准化研究
Erika Stefani Perez,Matheus Naia Fioretto,Bruna Tereza Thomazini Zanella et al.
Erika Stefani Perez et al.
Immunohistochemistry (IHC) is a widely used technique for the identification, localization, and analysis of protein expression in tissues, playing a critical role in pathological diagnosis and validation of data derived from molecular biolo...
Zebrafish Embryos to Profile Nano(bio)materials: A Modular Platform for Developmental Toxicity, Neurotoxicity, and Inflammation-Regeneration Assays [0.03%]
斑马鱼胚胎用于纳米（生物）材料特征分析：一个模块化平台，用于发育毒性、神经毒性和炎症再生试验
Cinzia Bragato,Alessandra Lama,Greta Carbone Faccin et al.
Cinzia Bragato et al.
Zebrafish embryos provide a rapid, low-cost in vivo platform to profile the developmental, neurotoxic, and immunomodulatory effects of engineered nano(bio)materials within a single vertebrate system. This article presents a modular workflow...
William J Jones,Rhea Couper,Gwyn W Gould et al.
William J Jones et al.
Obesity and type 2 diabetes (T2D) have reached epidemic proportions worldwide, affecting millions of people and contributing to serious health complications, such as metabolic syndrome, cardiovascular disease, and cancer. Adipose tissue is ...
Enhancing Biological Activity in Skin Repair with a Transdermal Peptide-Recombinant Type III Collagen Hydrogel [0.03%]
一种经皮肽-重组III型胶原水凝胶在皮肤修复中的促活性研究
Yue Liu,Jingjun Hong
Yue Liu
Skin wound healing is a complex biological process requiring coordinated cellular responses and extracellular matrix remodeling. Type III collagen (COL3A) plays a critical role in early-stage tissue repair, but its direct application is lim...
High-Content Immunofluorescence Quantification of Transcription Factors: Application to pSMADs for BMP/TGFβ Signaling Pathways [0.03%]
高内涵免疫荧光定量测定转录因子：在骨形态发生蛋白/转化生长因子β信号途径中pSMADs的应用
Valia Khodr,Paul Machillot,Catherine Picart
Valia Khodr
High-throughput immunofluorescence (IF) detection has become an essential tool in drug discovery for analyzing multiple markers and providing insights into cellular pathways. While IF techniques have been established for decades, high-conte...
MccB-catalyzed C-terminal Thioesterification for Protein Bioconjugation [0.03%]
McCB催化的蛋白质C末端硫酯化反应及其生物偶联应用
Di Yang,Amy M Weeks
Di Yang
Protein bioconjugation enables the installation of functional groups, probes, and payloads that cannot be genetically encoded, thereby enabling the study and manipulation of biological systems. An important class of intermediates in protein...