Transposon and Transgene Tribulations in Mosquitoes: A Perspective of piRNA Proportions [0.03%]
蚊子中的转座子和转基因问题：piRNA比例的视角
Nelson C Lau,Vanessa M Macias
Nelson C Lau
Mosquitoes, like Drosophila, are dipterans, the order of "true flies" characterized by a single set of two wings. Drosophila are prime model organisms for biomedical research, while mosquito researchers struggle to establish robust molecula...
Efficient Elimination of mtDNA from Mammalian Cells with 2',3'-Dideoxycytidine [0.03%]
2'，3'-二脱氧胞苷有效清除哺乳动物细胞中的线粒体DNA
Natalya Kozhukhar,Mikhail F Alexeyev
Natalya Kozhukhar
Mammalian cell lines devoid of mitochondrial DNA (mtDNA) are indispensable in studies aimed at elucidating the contribution of mtDNA to various cellular processes or interactions between nuclear and mitochondrial genomes. However, the reper...
Kinetic Studies on the 2-Oxoglutarate/Fe(II)-Dependent Nucleic Acid Modifying Enzymes from the AlkB and TET Families [0.03%]
阿尔肯B和TET家族的依赖于α-酮戊二酸/铁(II)的核酸修饰酶的动力学研究
Zhiyuan Peng,Jian Ma,Christo Z Christov et al.
Zhiyuan Peng et al.
Nucleic acid methylations are important genetic and epigenetic biomarkers. The formation and removal of these markers is related to either methylation or demethylation. In this review, we focus on the demethylation or oxidative modification...
The Effects of Particle LET and Fluence on the Complexity and Frequency of Clustered DNA Damage [0.03%]
粒子LET和通量对簇状DNA损伤的复杂性和频率的影响
Mohammad Rezaee,Amitava Adhikary
Mohammad Rezaee
Motivation: Clustered DNA-lesions are predominantly induced by ionizing radiation, particularly by high-LET particles, and considered as lethal damage. Quantification of this specific type of damage as a function of radia...
Mikhail Alexeyev
Mikhail Alexeyev
Disease-Associated Mutation A554V Disrupts Normal Autoinhibition of DNMT1 [0.03%]
疾病相关的基因变异A554V扰乱DNA甲基转移酶1的正常自我抑制状态
Rebecca L Switzer,Zach J Hartman,Geoffrey R Hewett et al.
Rebecca L Switzer et al.
DNA methyltransferase 1 (DNMT1) is the enzyme primarily responsible for propagation of the methylation pattern in cells. Mutations in DNMT1 have been linked to the development of adult-onset neurodegenerative disorders; these disease-associ...
An Enzyme-Linked Immunosorbent Assay for the Detection of Mitochondrial DNA-Protein Cross-Links from Mammalian Cells [0.03%]
一种检测哺乳动物细胞线粒体DNA-蛋白质交联的酶联免疫吸附测定法
Wenyan Xu,Linlin Zhao
Wenyan Xu
DNA-Protein cross-links (DPCs) are cytotoxic DNA lesions with a protein covalently bound to the DNA. Although much has been learned about the formation, repair, and biological consequences of DPCs in the nucleus, little is known regarding m...
Size- and Stereochemistry-Dependent Transcriptional Bypass of DNA Alkyl Phosphotriester Adducts in Mammalian Cells [0.03%]
哺乳动物细胞中DNA烷基磷三酯加合物转录旁路的尺寸和立体化学依赖性
Ying Tan,Jiabin Wu,Garrit Clabaugh et al.
Ying Tan et al.
Environmental, endogenous and therapeutic alkylating agents can react with internucleotide phosphate groups in DNA to yield alkyl phosphotriester (PTE) adducts. Alkyl-PTEs are induced at relatively high frequencies and are persistent in mam...
Inna Shokolenko,Mikhail Alexeyev
Inna Shokolenko
In the course of its short history, mitochondrial DNA (mtDNA) has made a long journey from obscurity to the forefront of research on major biological processes. mtDNA alterations have been found in all major disease groups, and their signif...
Jac A Nickoloff,Neelam Sharma,Lynn Taylor et al.
Jac A Nickoloff et al.
DNA replication stress is a constant threat that cells must manage to proliferate and maintain genome integrity. DNA replication stress responses, a subset of the broader DNA damage response (DDR), operate when the DNA replication machinery...