Use of paired Cas9-NG nickase and truncated sgRNAs for single-nucleotide microbial genome editing [0.03%]
利用Cas9-NG切酶和截短sgRNA进行微生物单碱基基因组编辑
Song Hee Jeong,Ho Joung Lee,Sang Jun Lee
Song Hee Jeong
The paired nickases approach, which utilizes clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) nickase and dual guide RNA, has the advantage of reducing off-target effects by being able to d...
Making gene editing accessible in resource limited environments: recommendations to guide a first-time user [0.03%]
在资源有限的环境中进行基因编辑：指导初次使用者的建议
Shivani Goolab,Janine Scholefield
Shivani Goolab
The designer nuclease, CRISPR-Cas9 system has advanced the field of genome engineering owing to its programmability and ease of use. The application of these molecular scissors for genome engineering earned the developing researchers the No...
Global regulatory policies for animal biotechnology: overview, opportunities and challenges [0.03%]
动物生物技术的全球监管政策：概况、机遇与挑战
Diane Wray-Cahen,Eric Hallerman,Mark Tizard
Diane Wray-Cahen
Genome editing (GnEd) has the potential to provide many benefits to animal agriculture, offering a means for achieving rapid growth, disease resistance, and novel phenotypes. The technology has the potential to be useful for rapidly incorpo...
The 4Fs of cotton: genome editing of cotton for fiber, food, feed, and fuel to achieve zero hunger [0.03%]
棉花的4F：基因组编辑实现零饥饿以提高棉花纤维、粮食、饲料和燃料品质
Muhammad Sulyman Saleem,Sultan Habibullah Khan,Aftab Ahmad et al.
Muhammad Sulyman Saleem et al.
Cotton is globally known for its high-priority cellulose-rich natural fiber. In addition to providing fiber for the textile industry, it is an important source material for edible oil, livestock feed, and fuel products. Global warming and t...
In vivo liver targeted genome editing as therapeutic approach: progresses and challenges [0.03%]
体内肝脏特异性基因组编辑治疗进展与挑战
Chiara Simoni,Elena Barbon,Andrés F Muro et al.
Chiara Simoni et al.
The liver is an essential organ of the body that performs several vital functions, including the metabolism of biomolecules, foreign substances, and toxins, and the production of plasma proteins, such as coagulation factors. There are hundr...
Genetic engineering and genome editing technologies as catalyst for Africa's food security: the case of plant biotechnology in Nigeria [0.03%]
基因工程和基因组编辑技术作为非洲粮食安全的催化剂：尼日利亚植物生物技术案例分析
Muyiwa Seyi Adegbaju,Titilayo Ajose,Ifeoluwa Elizabeth Adegbaju et al.
Muyiwa Seyi Adegbaju et al.
Many African countries are unable to meet the food demands of their growing population and the situation is worsened by climate change and disease outbreaks. This issue of food insecurity may lead to a crisis of epic proportion if effective...
Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production [0.03%]
通过基因编辑提高作物抗病性：增强农业生产的一种前景光明的方法
Subaya Manzoor,Sajad Un Nabi,Tariq Rasool Rather et al.
Subaya Manzoor et al.
Modern agriculture has encountered several challenges in achieving constant yield stability especially due to disease outbreaks and lack of long-term disease-resistant crop cultivars. In the past, disease outbreaks in economically important...
Horizon scanning of potential environmental applications of terrestrial animals, fish, algae and microorganisms produced by genetic modification, including the use of new genomic techniques [0.03%]
陆生动物、水产动物、藻类和微生物（包括采用新的基因技术产生的）的潜在环境影响的扫描筛查研究报告
Marianne Miklau,Sarah-Joe Burn,Michael Eckerstorfer et al.
Marianne Miklau et al.
With scientific progress and the development of new genomic techniques (NGTs), the spectrum of organisms modified for various purposes is rapidly expanding and includes a wide range of taxonomic groups. An improved understanding of which ne...
The potential of genome editing to create novel alleles of resistance genes in rice [0.03%]
利用基因组编辑技术在水稻中创制新型抗性等位基因的潜能
Pankaj Kumar Singh,Basavantraya N Devanna,Himanshu Dubey et al.
Pankaj Kumar Singh et al.
Rice, a staple food for a significant portion of the global population, faces persistent threats from various pathogens and pests, necessitating the development of resilient crop varieties. Deployment of resistance genes in rice is the best...
A gene edited pig model for studying LGR5+ stem cells: implications for future applications in tissue regeneration and biomedical research [0.03%]
一种用于研究LGR5+干细胞的基因编辑猪模型及其在组织再生和生物医学研究中的未来应用展望
Amanda B T Hill,Yanet M Murphy,Kathryn M Polkoff et al.
Amanda B T Hill et al.
Recent advancements in genome editing techniques, notably CRISPR-Cas9 and TALENs, have marked a transformative era in biomedical research, significantly enhancing our understanding of disease mechanisms and helping develop novel therapies. ...