From recalcitrance to precision: a robust regeneration, transformation and targeted gene editing framework in Cajanus cajan [0.03%]
从难缠到精准：热带豆(Cajanus cajan)的高效再生、遗传转化及基因组编辑体系建立
Rachana Verma,Jyotsna Bharti,Arulprakash Thangaraj et al.
Rachana Verma et al.
Pigeonpea (Cajanus cajan (L.) Millsp.; 2n = 2× = 22) is a drought-tolerant perennial grain legume commonly cultivated in India's rain-fed and dry land zones, and it is a remarkable natural source of minerals and protein worldwide. Despite ...
Clinical outcome of an SCNT-derived MSTN knockout buffalo: a case study [0.03%]
体细胞克隆去肌苷基因水牛的临床研究案例分析
Priyanka Singh,Kartikey Patel,Gaurav Tripathi et al.
Priyanka Singh et al.
Myostatin (MSTN) gene knockout has attracted considerable interest for enhancing meat production in livestock species due to its well-established role as a negative regulator of skeletal muscle growth. In this study, we report the clinical ...
CRISPR and Fanzor in sickle cell disease: current progress and future prospects [0.03%]
基因编辑和法佐尔在镰状细胞病中的研究进展及未来前景
Aisha Yousef Alhumoudi,Aminah Ghazi Alotaibi,Nada Fahad Alosaimi et al.
Aisha Yousef Alhumoudi et al.
Advancements in genome editing have established a new frontier for the treatment of various genetic diseases, including sickle cell disease (SCD). SCD, the most prevalent monogenic blood disorder, causes severe pain, organ damage, and reduc...
A high-throughput, streamlined cloning protocol to generate guide RNAs for CRISPR activation [0.03%]
一种用于CRISPR激活的高通量、精简型克隆协议以生成指导RNA
Shusen Zhu,Aura A Tamez González,Abdelrahman Alokda et al.
Shusen Zhu et al.
Caenorhabditis elegans is a powerful model for studying gene function and disease pathogenesis. While RNA interference effectively suppresses gene expression, CRISPR activation (CRISPRa) provides a robust tool for upregulating endogenous ge...
Reconstructing the complex architecture of the genome with molecular scissors: applying genome editing technology in precision medicine [0.03%]
利用分子剪刀重构复杂的基因组架构：在精准医学中应用基因编辑技术
Md Nur Amin Khan,Rohit Das,Pooja Barik et al.
Md Nur Amin Khan et al.
Our past, present, and future are governed by the grand design of the genetic blueprint, which was mapped more than two decades back. While the eloquent design of our genome results from millions of years of evolution and has reached a near...
Advances in gene editing for legume improvement: technologies, progress, and prospects [0.03%]
豆科作物基因编辑研究进展、现状及展望
Shallu Thakur,Shalini Pareek,G P Dixit et al.
Shallu Thakur et al.
Legumes are among the most important protein-rich crops in global agri-food systems. To meet the rising protein demand of a growing population, significant efforts are underway to enhance legume yield, nutritional quality, and resilience to...
Development of in planta genome editing by transient expression of genome-editing tools in tomato [0.03%]
通过瞬时表达基因组编辑工具进行植物体内基因组编辑的发展——以番茄为例
Misaki Kobayashi,Na Renhu,Shu Takahashi et al.
Misaki Kobayashi et al.
Two major processes are important for genome editing in plants: transformation by stable transfection, in which nucleic acids encoding genome-editing enzymes are introduced into plant cells and the regeneration of plant individuals from cel...
Editorial: Genome editing strategies for augmenting crop resilience against climate change [0.03%]
编辑部：增强作物抵御气候变化能力的基因组编辑策略
Wadzani Palnam Dauda,Amolkumar U Solanke,Ramesh Namdeo Pudake
Wadzani Palnam Dauda
Harnessing myostatin pleiotropy for multitrait improvement via precision gene editing [0.03%]
利用精准基因编辑挖掘肌生长抑制素多效性以开展肉牛多性状选育改善研究
Yajun Chen,Ruiyao Yang,Yucai Yang et al.
Yajun Chen et al.
The pursuit of sustainable livestock farming to meet the rising global protein demand has positioned myostatin (MSTN) gene editing as a key technology. However, the field's focus on the remarkable double-muscle phenotype has often overshado...