Cas9 endonuclease: a molecular tool for in vitro cloning and CRISPR edit detection [0.03%]
Cas9 核酸内切酶：体外克隆及 CRISPR 编辑检测的分子工具
Xingliang Ma,Dhouha Kthiri,Manpartik S Gill et al.
Xingliang Ma et al.
Large genetic engineering constructs often face limitations in DNA element addition or replacement due to lack of unique endonuclease recognition sites. Traditional restriction resistance methods can identify CRISPR-induced mutants efficien...
CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops [0.03%]
利用 CRISPR/Cas9 增强主要农作物的气候适应性是一项可持续的技术手段
Navjot Kaur,Muslim Qadir,Dali V Francis et al.
Navjot Kaur et al.
Climate change is a global concern for agriculture, food security, and human health. It affects several crops and causes drastic losses in yield, leading to severe disturbances in the global economy, environment, and community. The conseque...
Emerging applications of gene editing technologies for the development of climate-resilient crops [0.03%]
基因编辑技术在开发气候适应型作物中的新兴应用
R L Chavhan,S G Jaybhaye,V R Hinge et al.
R L Chavhan et al.
Climate change threatens global crop yield and food security due to rising temperatures, erratic rainfall, and increased abiotic stresses like drought, heat, and salinity. Gene editing technologies, including CRISPR/Cas9, base editors, and ...
CRISPR-mediated genome editing of wheat for enhancing disease resistance [0.03%]
利用CRISPR介导的基因组编辑提高小麦抗病性
Joshua Waites,V Mohan Murali Achary,Easter D Syombua et al.
Joshua Waites et al.
Wheat is cultivated across diverse global environments, and its productivity is significantly impacted by various biotic stresses, most importantly but not limited to rust diseases, Fusarium head blight, wheat blast, and powdery mildew. The...
Engineering tomato disease resistance by manipulating susceptibility genes [0.03%]
通过操纵易感基因来工程化提高番茄的抗病性
Duoduo Wang,Palash Mandal,Md Sazan Rahman et al.
Duoduo Wang et al.
Various pathogens severely threaten tomato yield and quality. Advances in understanding plant-pathogen interactions have revealed the intricate roles of resistance (R) and susceptibility (S) genes in determining plant immunity. While R gene...
Gӧtz Laible,Mark Cigan
Gӧtz Laible
Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells [0.03%]
高温抑制鸡原始生殖细胞的生物学特性和迁移能力
Yuzhou Gu,Kexin Wu,Bowen Niu et al.
Yuzhou Gu et al.
Primordial germ cells (PGCs) play a crucial role in transmitting genetic information to the next-generation. In chickens, genetically edited PGCs can be propagated in vitro and subsequently transplanted into recipient embryos to produce off...
Shujuan Meng,Aijun Miao,Sen Wu et al.
Shujuan Meng et al.
Protein drug production encompasses various methods, among which animal bioreactors are emerging as a transgenic system. Animal bioreactors have the potential to reduce production costs and increase efficiency, thereby producing recombinant...
Danling Hu,Lizhe Hu,Yaqiang Lu et al.
Danling Hu et al.
Virus-induced genome editing (VIGE) technologies have been developed to address the limitations to plant genome editing, which heavily relies on genetic transformation and regeneration. However, the application of VIGE in plants is hampered...
Transcriptional engineering for value enhancement of oilseed crops: a forward perspective [0.03%]
油料作物转录组工程的价值提升：展望未来
Charli Kaushal,Mahak Sachdev,Mansi Parekh et al.
Charli Kaushal et al.
Plant-derived oils provide 20%-35% of dietary calories and are a primary source of essential omega-6 (linoleic) and omega-3 (α-linolenic) fatty acids. While traditional breeding has significantly increased yields in key oilseed crops like ...