Long read sequencing reveals transgene concatemerization and vector sequences integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes [0.03%]
长读取测序揭示了小鼠受精卵中AAV电转CRISPR RNP复合物后转基因串联化和载体序列整合的现象
Muhammad W Luqman,Piroon Jenjaroenpun,Jessica Spathos et al.
Muhammad W Luqman et al.
Over the last decade CRISPR gene editing has been successfully used to streamline the generation of animal models for biomedical research purposes. However, one limitation to its use is the potential occurrence of on-target mutations that m...
Recent developments, challenges and opportunities in genome editing for crop science from a societal perspective [0.03%]
社会视角下作物科学的基因组编辑近年的发展、挑战和机遇
Srividhya Venkataraman,David Zaruk,Kathleen Hefferon
Srividhya Venkataraman
Genome editing has presented enormous potential in the fields of medicine and agriculture. Here, we explore the social and regulatory aspects of genome editing from the perspective of food security. We provide recent examples of crop genome...
Lentiviral CRISPRa/i in the adult prairie vole brain: modulating neuronal gene expression without DNA cleavage [0.03%]
成年草原田鼠脑内 lentiviral crisprai：无需dna断裂即可调节神经元基因表达
Meredith K Loth,Kendall T Mesch,Celine Herrera-Garcia et al.
Meredith K Loth et al.
Prairie voles (Microtus ochrogaster) are a powerful model for studying the neurobiology of social bonding, yet tools for region- and cell type-specific gene regulation remain underdeveloped in this species. Here, we present a lentivirus-med...
Joshua Yeboah Asiamah,Sakina Haruna Mahdi,Kusum R Tamang et al.
Joshua Yeboah Asiamah et al.
Throughout history, leguminous crops have contributed significantly to the human diet. Grain legumes have long been identified as a valuable nutritional source for humans. However, their significance extends beyond nutrition to global food ...
Editorial: Plant breeding innovations-CRISPR as a powerful weapon for agricultural crops [0.03%]
编辑来稿：植物育种创新技术——CRISPR将成为农作物改良的有力武器
Basavantraya N Devanna,Yugander Arra,Maganti Sheshu Madhav
Basavantraya N Devanna
Base editors in zebrafish: a new era for functional genomics and disease modeling [0.03%]
基于贝斯编辑器的斑马鱼：功能基因组学和疾病建模的新时代
Yuwen Liu,Chao Li,Yiren Qiu et al.
Yuwen Liu et al.
Base editing has revolutionized genome engineering by enabling precise single-nucleotide modifications without inducing double-strand breaks. As a powerful and efficient gene-editing tool, base editors (BEs) have been widely applied in vari...
Efficient GBA1 editing via HDR with ssODNs by outcompeting pseudogene-mediated gene conversion upon CRISPR/Cas9 cleavage [0.03%]
CRISPR/Cas9切割时通过ssODNs进行HDR可有效编辑GBA1基因，并能竞争假基因介导的基因转换效应
Joseph S Lagas,Monica F Sentmanat,Xiaoxia Cui
Joseph S Lagas
Introduction: CRISPR/Cas9-edited induced pluripotent stem cells (iPSCs) are valuable research models for mechanistic studies. However, gene conversion between a gene-pseudogene pair that share high sequence identity and f...
Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology [0.03%]
基因组学的未来展望：CRISPR、单细胞组学及癌症和免疫学中的应用
A Vipin Menon,Bicna Song,Lumen Chao et al.
A Vipin Menon et al.
The CRISPR system has transformed many research areas, including cancer and immunology, by providing a simple yet effective genome editing system. Its simplicity has facilitated large-scale experiments to assess gene functionality across di...
Mapping the therapeutic landscape of CRISPR-Cas9 for combating age-related diseases [0.03%]
绘制CRISPR-Cas9治疗年龄相关疾病图谱
Qiyu He,Yida Wang,Zhimin Tan et al.
Qiyu He et al.
CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated protein 9) has emerged as a transformative genome-editing tool with significant therapeutic potential for age-related diseases, including Alzheimer's disease,...
CRISPR-dependent base editing as a therapeutic strategy for rare monogenic disorders [0.03%]
基于CRISPR的碱基编辑疗法在单基因罕见病中的应用策略研究
Júlia-Jié Cabré-Romans,Raquel Cuella-Martin
Júlia-Jié Cabré-Romans
Rare monogenic disorders are caused by mutations in single genes and have an incidence rate of less than 0.5%. Due to their low prevalence, these diseases often attract limited research and commercial interest, leading to significant unmet ...