Stem cell and CRISPR/Cas9 gene editing technology in Alzheimer's disease therapy: from basic research to clinical innovation [0.03%]
阿尔茨海默病治疗中的干细胞和CRISPR/Cas9基因编辑技术：从基础研究到临床创新
Cong He,Baojiang Chen,Ciai Yan et al.
Cong He et al.
Alzheimer's disease (AD), a progressive neurodegenerative disorder characterized by Aβ plaques, tau protein neuronal fiber tangles, and neuroinflammation, poses a significant global health problem, and current therapies focus on the sympto...
Ethical dimensions and societal implications: ensuring the social responsibility of CRISPR technology [0.03%]
伦理维度及社会影响：确保CRISPR技术的社会责任
Irfan Biswas
Irfan Biswas
CRISPR-Cas9 is a breakthrough genome-editing platform that can cut chosen DNA sequences with unprecedented speed, accuracy, and affordability. By reprogramming a single guide RNA, researchers now alter gene function, correct pathogenic vari...
Shakeel Ahmad,Iqrar Ahmad Rana,Kevin M Folta et al.
Shakeel Ahmad et al.
CATS: a bioinformatic tool for automated Cas9 nucleases activity comparison in clinically relevant contexts [0.03%]
cats：一种_bioinformatics_工具，可自动比较临床相关的Cas9内切酶活性
Ettore Rocchi,Federico Magnani,Gastone Castellani et al.
Ettore Rocchi et al.
Introduction: With the growing number of Cas9 nucleases available to genetic engineers, selecting the most suitable one for a given application can be challenging. A major complication arises from the differing protospace...
CRISPR-Cas9 editing of TNFAIP3 variants in salivary gland epithelial cells to study Sjögren's disease pathogenesis [0.03%]
使用CRISPR-Cas9编辑唾液腺上皮细胞中的TNFAIP3变异以研究Sjög伦综合征发病机制
Subhashis Ghosh,Qisheng Tu,Zoe Xiaofang Zhu et al.
Subhashis Ghosh et al.
Sjögren's disease (SD) is a systemic autoimmune disease that particularly affects the salivary and lacrimal glands, causing sicca symptoms. Genetic polymorphism in the TNFAIP3 gene has been implicated in the pathogenesis of SD. In this stu...
Simply cut out - Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion in Trichoderma atroviride [0.03%]
简单的基因删除-在青霉菌黑色变种中结合CRISPR / Cas9 RNP和瞬时选择端粒载体进行无标记基因删除
Mario Gründlinger,Chiara Ellensohn,Leo Drechsel et al.
Mario Gründlinger et al.
Trichoderma atroviride is a well-known mycoparasitic fungus widely used for the biological control of fungal plant pathogens. However, genetic manipulation in this organism remains challenging due to the limited availability of versatile an...
Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat [0.03%]
高效基因组编辑的单碱基编辑器（SDN1-CRISPR / Cas9）gRNA设计方法学研究小麦中的应用
Tushadri Singh,H M Mamrutha,Rajender Singh et al.
Tushadri Singh et al.
Background: CRISPR/Cas9 technology has gained popularity due to its efficient, widely applicable, and relatively easy genome editing. Furthermore, the removal of regulation on site-directed nuclease1- (SDN1) and SDN2-deve...
Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation [0.03%]
基因编辑在秘鲁可持续农业中的应用：进展、潜在用途和规范制定
Marilu Mestanza,Angel David Hernández-Amasifuen,Alexandra Jherina Pineda-Lázaro et al.
Marilu Mestanza et al.
Peruvian agriculture is characterize by crops such as potato, maize, rice, asparagus, mango, banana, avocado, cassava, onion, oil palm, chili, papikra, blueberry, coffee, cacao, grapes, quinoa, olive, citrus and others. All of them have cha...
DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection [0.03%]
通过RNP介导的转染在覆盆子（红悬钩子）原生质体中进行免DNA CRISPR基因组编辑
Ryan Creeth,Andrew Thompson,Zoltan Kevei
Ryan Creeth
Protoplast-based systems have been utilised in a wide variety of plant species to enable genome editing without chromosomal introgression of foreign DNA into plant genomes. DNA-free genome editing followed by protoplast regeneration allows ...
A long shelf-life melon created via CRISPR/Cas9 RNP-based in planta genome editing [0.03%]
一种通过CRISPR/Cas9 RNP介导的植物体内基因组编辑培育的耐储运甜瓜品种
Kentaro Sasaki,Kaoru Urano,Naozumi Mimida et al.
Kentaro Sasaki et al.
Genome editing in melon (Cucumis melo L.) remains a significant challenge due to the inefficiencies associated with conventional cell culture-based transformation methods. In the present study, a novel in planta Particle Bombardment (iPB) a...