Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants [0.03%]
植物质粒载体的先导编辑介导的拼写改变和荧光标记
Li Wang,Hilal Betul Kaya,Ning Zhang et al.
Li Wang et al.
Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allo...
Baboon Envelope Pseudotyped "Nanoblades" Carrying Cas9/gRNA Complexes Allow Efficient Genome Editing in Human T, B, and CD34+ Cells and Knock-in of AAV6-Encoded Donor DNA in CD34+ Cells [0.03%]
携带Cas9/gRNA复合物的狒狒包膜假型“纳米刀”可实现人类T细胞，B细胞和CD34+细胞的有效基因编辑以及CD34+细胞中AAV6编码供体DNA的敲入
Alejandra Gutierrez-Guerrero,Maria Jimena Abrey Recalde,Philippe E Mangeot et al.
Alejandra Gutierrez-Guerrero et al.
Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells and living organisms. However, their delivery into human blood cells can be challenging. Here, we have utilized "nanoblades," a new technology t...
Genome Editing and Protoplast Regeneration to Study Plant-Pathogen Interactions in the Model Plant Nicotiana benthamiana [0.03%]
基因编辑和原生质体再生研究模式植物本氏烟草中的植物-病原互作关系
Chen-Tran Hsu,Wen-Chi Lee,Yu-Jung Cheng et al.
Chen-Tran Hsu et al.
Biotic diseases cause substantial agricultural losses annually, spurring research into plant pathogens and strategies to mitigate them. Nicotiana benthamiana is a commonly used model plant for studying plant-pathogen interactions because it...
Non-immunogenic Induced Pluripotent Stem Cells, a Promising Way Forward for Allogenic Transplantations for Neurological Disorders [0.03%]
非免疫原性诱导多能干细胞，神经性疾病同种异体移植的一种前景广阔的方法
Henriette Reventlow Frederiksen,Ulrik Doehn,Pernille Tveden-Nyborg et al.
Henriette Reventlow Frederiksen et al.
Neurological disorder is a general term used for diseases affecting the function of the brain and nervous system. Those include a broad range of diseases from developmental disorders (e.g., Autism) over injury related disorders (e.g., strok...
Development of a Transformable Fast-Flowering Mini-Maize as a Tool for Maize Gene Editing [0.03%]
可转换的快开花迷你玉米的开发作为基因编辑工具在玉米中的应用
Morgan E McCaw,Keunsub Lee,Minjeong Kang et al.
Morgan E McCaw et al.
Maize (Zea mays ssp. mays) is a popular genetic model due to its ease of crossing, well-established toolkits, and its status as a major global food crop. Recent technology developments for precise manipulation of the genome are further impa...
Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility [0.03%]
使用高效且扩展了PAM兼容性的CRISPR-xCas9工具组进行植物基因组编辑
Chengwei Zhang,Guiting Kang,Xinxiang Liu et al.
Chengwei Zhang et al.
The CRISPR-Cas9 system enables simple, rapid, and effective genome editing in many species. Nevertheless, the requirement of an NGG protospacer adjacent motif (PAM) for the widely used canonical Streptococcus pyogenes Cas9 (SpCas9) limits t...
Mesenchymal Stromal Cells as a Cellular Target in Myeloid Malignancy: Chances and Challenges in the Genome Editing of Stromal Alterations [0.03%]
间充质基质细胞作为髓系恶性肿瘤的细胞靶点：基因组编辑在基质改变中的机遇与挑战
Bella Banjanin,Rebekka K Schneider
Bella Banjanin
The contribution of bone marrow stromal cells to the pathogenesis and therapy response of myeloid malignancies has gained significant attention over the last decade. Evidence suggests that the bone marrow stroma should not be neglected in t...
Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems in Mammalian Cells [0.03%]
利用合成型引导RNA以增强哺乳动物细胞中的CRISPR基因组编辑系统
Daniel Allen,Michael Rosenberg,Ayal Hendel
Daniel Allen
CRISPR-Cas9 is quickly revolutionizing the way we approach gene therapy. CRISPR-Cas9 is a complexed, two-component system using a short guide RNA (gRNA) sequence to direct the Cas9 endonuclease to the target site. Modifying the gRNA indepen...
A Small Key for a Heavy Door: Genetic Therapies for the Treatment of Hemoglobinopathies [0.03%]
沉重之门的小钥匙：治疗血红蛋白病的基因疗法
Hidde A Zittersteijn,Cornelis L Harteveld,Stefanie Klaver-Flores et al.
Hidde A Zittersteijn et al.
Throughout the past decades, the search for a treatment for severe hemoglobinopathies has gained increased interest within the scientific community. The discovery that ɤ-globin expression from intact HBG alleles complements defective HBB a...
Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants [0.03%]
通过基因定位和随后的单链退火介导的标记基因切除实现植物microRNA靶点位点的精确基因组编辑
Namie Ohtsuki,Keiko Kizawa,Akiko Mori et al.
Namie Ohtsuki et al.
Gene targeting (GT) enables precise genome modification-e.g., the introduction of base substitutions-using donor DNA as a template. Combined with clean excision of the selection marker used to select GT cells, GT is expected to become a sta...