Genome editing for improving nutritional quality, post-harvest shelf life and stress tolerance of fruits, vegetables, and ornamentals [0.03%]
基因组编辑改善果蔬和观赏作物的营养品质、采后贮运性状及抗逆性的研究进展
Punam Sharma,Anuradha Pandey,Rinku Malviya et al.
Punam Sharma et al.
Agricultural production relies on horticultural crops, including vegetables, fruits, and ornamental plants, which sustain human life. With an alarming increase in human population and the consequential need for more food, it has become nece...
In search of an ideal template for therapeutic genome editing: A review of current developments for structure optimization [0.03%]
寻找理想的治疗性基因组编辑模板：结构优化的当前进展综述
Alena Shakirova,Timofey Karpov,Yaroslava Komarova et al.
Alena Shakirova et al.
Gene therapy is a fast developing field of medicine with hundreds of ongoing early-stage clinical trials and numerous preclinical studies. Genome editing (GE) now is an increasingly important technology for achieving stable therapeutic effe...
T-CAST: An optimized CAST-Seq pipeline for TALEN confirms superior safety and efficacy of obligate-heterodimeric scaffolds [0.03%]
基于TALEN的优化型CAST-Seq管道（T-Cast）确认了义务异二聚体支架具有更优的安全性与有效性
Manuel Rhiel,Kerstin Geiger,Geoffroy Andrieux et al.
Manuel Rhiel et al.
Transcription activator-like effector nucleases (TALENs) are programmable nucleases that have entered the clinical stage. Each subunit of the dimer consists of a DNA-binding domain composed of an array of TALE repeats fused to the catalytic...
Brigitte Voigt
Brigitte Voigt
This article presents a proposal on how the European Union's regulatory framework on genetically modified (GM) plants should be reformed in light of recent developments in genomic plant breeding techniques. The reform involves a three-tier ...
Rare immune diseases paving the road for genome editing-based precision medicine [0.03%]
罕见免疫性疾病为基于基因编辑的精准医疗开辟道路
Mara Pavel-Dinu,Simon Borna,Rosa Bacchetta
Mara Pavel-Dinu
Clustered regularly interspaced short palindromic repeats (CRISPR) genome editing platform heralds a new era of gene therapy. Innovative treatments for life-threatening monogenic diseases of the blood and immune system are transitioning fro...
CRISPR/Cas9 mediated gene editing in non-model nematode Panagrolaimus sp. PS1159 [0.03%]
基于CRISPR/Cas9的非模型线虫Panagrolaimus sp. PS1159基因编辑技术
Viktoria Hellekes,Denise Claus,Johanna Seiler et al.
Viktoria Hellekes et al.
The phylum Nematoda harbors a huge diversity of species in a broad range of ecosystems and habitats. Nematodes share a largely conserved Bauplan but major differences have been found in early developmental processes. The development of the ...
Corrigendum: CRISPR nuclease off-target activity and mitigation strategies [0.03%]
Correction: CRISPR核酸酶脱靶效应及缓解策略
Beeke Wienert,M Kyle Cromer
Beeke Wienert
[This corrects the article DOI: 10.3389/fgeed.2022.1050507.]. Keywords: CRISPR/Cas9; gene therapy; genome ed...
Published Erratum
Frontiers in genome editing. 2023 Jan 20:4:1112956. DOI:10.3389/fgeed.2022.1112956 2023
On-site and visual detection of sorghum mosaic virus and rice stripe mosaic virus based on reverse transcription-recombinase-aided amplification and CRISPR/Cas12a [0.03%]
基于反转录重组酶介导扩增和CRISPR/Cas12a的高粱花叶病毒及水稻条纹花叶病毒现场可视化检测技术
Junkai Wang,Xiuqin Huang,Siping Chen et al.
Junkai Wang et al.
Rapid, sensitive and visual detection of plant viruses is conducive to effective prevention and control of plant viral diseases. Therefore, combined with reverse transcription and recombinase-aided amplification, we developed a CRISPR/Cas12...
Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits [0.03%]
CCR5的CRISPR-Cas9基因编辑的毛里求斯食蟹猴胚胎细胞的全基因组测序揭示了大规模的缺失和脱靶效应
Jenna Kropp Schmidt,Yun Hee Kim,Nick Strelchenko et al.
Jenna Kropp Schmidt et al.
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications. Nonhuman primates are physiologically closer to humans than other labor...
Genome editing approaches using reproductive cells/tissues in flowering plants [0.03%]
开花植物利用生殖细胞/组织的基因组编辑方法
Erika Toda,Norio Kato,Tetsuya Higashiyama et al.
Erika Toda et al.
Targeted mutagenesis via programmable nucleases including the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system has been broadly utilized to generate genome-edited or...