Editorial: CRISPR and beyond: Cutting-edge technologies for gene correction in therapeutic applications [0.03%]
editorial: crispr和更先进的技术在治疗性基因修正中的应用
Ayal Hendel,Rasmus O Bak
Ayal Hendel
Contamination of imported kernels by unapproved genome-edited varieties poses a major challenge for monitoring and traceability during transport and handling on a global scale: inferences from a study on feral oilseed rape in Austria [0.03%]
进口种子被未经批准的基因编辑品种污染对全球运输和处理过程中的监测和追溯构成重大挑战：奥地利野生油菜研究启示
Kathrin Pascher,Christa Hainz-Renetzeder,Michaela Jagersberger et al.
Kathrin Pascher et al.
Novel techniques such as CRISPR/Cas are increasingly being applied for the development of modern crops. However, the regulatory framework for production, labelling and handling of genome-edited organisms varies worldwide. Currently, the Eur...
Assessment of anti-HIV-1 guide RNA efficacy in cells containing the viral target sequence, corresponding gRNA, and CRISPR/Cas9 [0.03%]
评估含有HIV-1靶序列、相应导向RNA和CRISPR/Cas9的细胞中的抗HIV-1导向RNA的效力
Alexander G Allen,Cheng-Han Chung,Stephen D Worrell et al.
Alexander G Allen et al.
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 gene editing system has been shown to be effective at inhibiting human immunodeficiency virus type 1 (HIV-1). Studies have not consistently used a trackable dual re...
CRISPR/Cas9-generated mouse model with humanizing single-base substitution in the Gnao1 for safety studies of RNA therapeutics [0.03%]
用于RNA药物安全性研究的具有人类GNAO1单碱基突变的CRISPR/Cas9小鼠模型
Anna V Polikarpova,Tatiana V Egorova,Evgenii A Lunev et al.
Anna V Polikarpova et al.
The development of personalized medicine for genetic diseases requires preclinical testing in the appropriate animal models. GNAO1 encephalopathy is a severe neurodevelopmental disorder caused by heterozygous de novo mutations in the GNAO1 ...
Corrigendum: Establishment of an efficient protoplast regeneration and transfection protocol for field cress (Lepidium campestre) [0.03%]
拟南芥近缘种田旋花原生质体转染和再生体系的建立及更正
Sjur Sandgrind,Xueyuan Li,Emelie Ivarson et al.
Sjur Sandgrind et al.
[This corrects the article DOI: 10.3389/fgeed.2021.757540.]. Keywords: Braccicaceae; CRISPR/Cas9; domesticat...
Published Erratum
Frontiers in genome editing. 2023 Mar 27:5:1183791. DOI:10.3389/fgeed.2023.1183791 2023
Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice [0.03%]
水稻中Cas9同源物、Cas12a核酸酶和温度的编辑效率
Eudald Illa-Berenguer,Peter R LaFayette,Wayne A Parrott
Eudald Illa-Berenguer
The advent of CRISPR-Cas technology has made it the genome editing tool of choice in all kingdoms of life, including plants, which can have large, highly duplicated genomes. As a result, finding adequate target sequences that meet the speci...
Editorial: Genome and transcriptome editing to understand and treat neuromuscular diseases [0.03%]
述评：基因组和转录组的编辑在神经肌肉疾病研究和治疗中的应用
Rika Maruyama,Alyson Fiorillo,Christopher Heier et al.
Rika Maruyama et al.
Genome editing in rice mediated by miniature size Cas nuclease SpCas12f [0.03%]
由微型SpCas12f核糖核酸酶介导的水稻基因组编辑
Satoru Sukegawa,Osamu Nureki,Seiichi Toki et al.
Satoru Sukegawa et al.
Cas9 derived from Streptococcus pyogenes (SpCas9) is used widely in genome editing using the CRISPR-Cas system due to its high activity, but is a relatively large molecule (1,368 amino acid (a.a.) residues). Recently, targeted mutagenesis i...
High-efficiency editing in hematopoietic stem cells and the HUDEP-2 cell line based on in vitro mRNA synthesis [0.03%]
基于体外mRNA合成在造血干细胞和HUDEP-2细胞系中的高效编辑
Nikoletta Y Papaioannou,Petros Patsali,Basma Naiisseh et al.
Nikoletta Y Papaioannou et al.
Introduction: Genome editing tools, such as CRISPR/Cas, TALE nucleases and, more recently, double-strand-break-independent editors, have been successfully used for gene therapy and reverse genetics. Among various challenges in the field, to...
Prime editing in hematopoietic stem cells-From ex vivo to in vivo CRISPR-based treatment of blood disorders [0.03%]
造血干细胞中的 prime 编辑技术：基于 CRISPR 的血液疾病离体和原位治疗
Jonas Holst Wolff,Jacob Giehm Mikkelsen
Jonas Holst Wolff
Prime editing of human hematopoietic stem cells has the potential to become a safe and efficient way of treating diseases of the blood directly in patients. By allowing site-targeted gene intervention without homology-directed repair donor ...