Editorial: Genome editing for agricultural sustainability: developments in tools, potential applications, and regulatory policy [0.03%]
基因编辑促进农业可持续发展：研究工具、潜在应用及政策制定方面的进展
Felicity J Keiper,Thorben Sprink,Ian Douglas Godwin
Felicity J Keiper
CRISPR gene editing to improve crop resistance to parasitic plants [0.03%]
利用CRISPR基因编辑改善农作物对寄生植物的抗性
Min-Yao Jhu,Evan E Ellison,Neelima R Sinha
Min-Yao Jhu
Parasitic plants pose a significant threat to global agriculture, causing substantial crop losses and hampering food security. In recent years, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) gene-editing technology has e...
Characterization of transcriptional enhancers in the chicken genome using CRISPR-mediated activation [0.03%]
利用CRISPR激活技术表征鸡基因组中的转录增强子
Jeong Hoon Han,Hong Jo Lee,Tae Hyun Kim
Jeong Hoon Han
DNA regulatory elements intricately control when, where, and how genes are activated. Therefore, understanding the function of these elements could unveil the complexity of the genetic regulation network. Genome-wide significant variants ar...
CRISPR/Cas9 mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family [0.03%]
利用CRISPR/Cas9技术对拟南芥GROWTH-REGULATING FACTOR（GRF）基因家族进行突变分析
Juan Angulo,Christopher P Astin,Olivia Bauer et al.
Juan Angulo et al.
Genome editing in plants typically relies on T-DNA plasmids that are mobilized by Agrobacterium-mediated transformation to deliver the CRISPR/Cas machinery. Here, we introduce a series of CRISPR/Cas9 T-DNA vectors for minimal settings, such...
Hs1Cas12a and Ev1Cas12a confer efficient genome editing in plants [0.03%]
Hs1Cas12a和Ev1Cas12a植物高效基因组编辑
Gen Li,Yingxiao Zhang,Micah Dailey et al.
Gen Li et al.
Cas12a, also known as Cpf1, is a highly versatile CRISPR-Cas enzyme that has been widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a has unique features that make it a highly efficient genome editing tool at AT-r...
Konrad Fischer,Angelika Schnieke
Konrad Fischer
The first genetically modified large animals were developed in 1985 by microinjection to increase the growth of agricultural livestock such as pigs. Since then, it has been a difficult trail due to the lack of genetic tools. Although method...
Recalcitrance to transformation, a hindrance for genome editing of legumes [0.03%]
豆科植物基因组编辑难以实现的原因及应对策略
V M Nivya,Jasmine M Shah
V M Nivya
Plant genome editing, a recently discovered method for targeted mutagenesis, has emerged as a promising tool for crop improvement and gene function research. Many genome-edited plants, such as rice, wheat, and tomato, have emerged over the ...
Genome-wide CRISPR screens and their applications in infectious disease [0.03%]
基因组范围的CRISPR筛选及其在传染病学中的应用
Kaveri Srivastava,Bhaswati Pandit
Kaveri Srivastava
Inactivation or targeted disruption of a gene provides clues to assess the function of the gene in many cellular processes. Knockdown or knocking out a gene has been widely used for this purpose. However, recently CRISPR mediated genome edi...
Editorial: Genome edited organisms for agriculture-challenges and perspectives for development and regulation [0.03%]
基因组编辑农业生物的发展和监管面临的挑战及前景稷望杂交水稻奖获奖报告
Michael Eckerstorfer,Sarah Zanon Agapito-Tenfen,Gijs A Kleter
Michael Eckerstorfer
Current approaches and potential challenges in the delivery of gene editing cargos into hematopoietic stem and progenitor cells [0.03%]
造血干细胞和祖细胞基因编辑递送的现有方法及潜在挑战
Ramya Murugesan,Karthik V Karuppusamy,Srujan Marepally et al.
Ramya Murugesan et al.
Advancements in gene delivery and editing have expanded the applications of autologous hematopoietic stem and progenitor cells (HSPCs) for the treatment of monogenic and acquired diseases. The gene editing toolbox is growing, and the abilit...