Achieving simultaneous removal of carbon and nitrogen by an integrated process of anaerobic membrane bioreactor and flow-through biofilm reactor [0.03%]
一种集厌氧膜生物反应器和流动生物膜反应器于一体的碳氮同步去除工艺
Xueshen Wu,Chao Wang,Depeng Wang et al.
Xueshen Wu et al.
In this study, a combined system consisting of an anaerobic membrane bioreactor (AnMBR) and flow-through biofilm reactor/CANON (FTBR/CANON) was developed to simultaneously remove carbon and nitrogen from synthetic livestock wastewater. The ...
Exploring the diversity of microbes and natural products from fungus-growing termite tripartite symbiosis [0.03%]
探求菌圃白蚁三元共生体系中的微生物多样性及其次生代谢产物多样性
Muhammad Shoaib,Ruining Bai,Shuai Li et al.
Muhammad Shoaib et al.
The fungus-growing termite is considered a distinct ecological niche because it involves a tripartite symbiosis between the termite host, gut microflora, and the in vitro fungus Termitomyces, which has led to the expansion of highly organiz...
Identification and application of a strong bidirectional acmN2p promoter from actinomycin D-producing streptomycetes [0.03%]
来源于放线菌素D产生链霉菌的强双向acmN2p启动子的鉴定及应用
Sainan Li,Danfeng Tang,Xu Zhao et al.
Sainan Li et al.
Natural product biosynthesis is controlled at multiple levels. Characterization of naturally occurring promoters has facilitated the study of the synthetic biology of natural products. Herein, we report the discovery of two high-yield actin...
Advances and applications of CRISPR/Cas-mediated interference in Escherichia coli [0.03%]
CRISPR/Cas介导的基因干扰在大肠杆菌中的应用及研究进展
Xiaohui Lim,Congqiang Zhang,Xixian Chen
Xiaohui Lim
The bacterium Escherichia coli (E. coli) is one of the most widely used chassis microbes employed for the biosynthesis of numerous valuable chemical compounds. In the past decade, the metabolic engineering of E. coli has undergone significa...
A new bio-oxidation method for removing iron deposits from waterlogged wood of Nanhai I shipwreck, Guangdong, China [0.03%]
中国广东南海一号沉船木质文物铁腐蚀产物的生物氧化去除试验研究
Yishu Wang,Zijun Zhao,Jianqun Lin et al.
Yishu Wang et al.
The widespread presence of iron and sulfur compounds such as pyrite in marine waterlogged archeological wood (WAW) can cause irreversible damage to the safety of its preservation. This issue has been a longstanding concern for cultural heri...
Half a century after their discovery: Structural insights into exonuclease and annealase proteins catalyzing recombineering [0.03%]
发现后的半个世纪：重组过程中核酸外切酶和退火酶的结构洞察
Lucy J Fitschen,Timothy P Newing,Nikolas P Johnston et al.
Lucy J Fitschen et al.
Recombineering is an essential tool for molecular biologists, allowing for the facile and efficient manipulation of bacterial genomes directly in cells without the need for costly and laborious in vitro manipulations involving restriction e...
Engineering Saccharomyces cerevisiae for efficient production of recombinant proteins [0.03%]
工程改造酿酒酵母用于高效表达重组蛋白
Shuo Yang,Liyun Song,Jing Wang et al.
Shuo Yang et al.
Saccharomyces cerevisiae is an excellent microbial cell factory for producing valuable recombinant proteins because of its fast growth rate, robustness, biosafety, ease of operability via mature genomic modification technologies, and the pr...
Role of homologous recombination/recombineering on human adenovirus genome engineering: Not the only but the most competent solution [0.03%]
同源重组/基因精准编辑在人腺病毒全基因组工程中的作用：不是唯一但最胜任的解决方案
Lisa-Marie Dawson,Montaha Alshawabkeh,Katrin Schröer et al.
Lisa-Marie Dawson et al.
Adenoviruses typically cause mild illnesses, but severe diseases may occur primarily in immunodeficient individuals, particularly children. Recently, adenoviruses have garnered significant interest as a versatile tool in gene therapy, tumor...
Recent advances in genome-scale engineering in Escherichia coli and their applications [0.03%]
大肠杆菌基因组规模工程研究的最新进展及其应用
Hui Gao,Zhichao Qiu,Xuan Wang et al.
Hui Gao et al.
Owing to the rapid advancement of genome engineering technologies, the scale of genome engineering has expanded dramatically. Genome editing has progressed from one genomic alteration at a time that could only be employed in few species, to...
Rational design of a highly active N-glycosyltransferase mutant using fragment replacement approach [0.03%]
基于片段替换的高活性N-糖基转移酶突变体理性设计
Jiangyu Yang,Kun Li,Yongheng Rong et al.
Jiangyu Yang et al.
The modularity of carbohydrate-active enzymes facilitates that enzymes with different functions have similar fragments. However, because of the complex structure of the enzyme active sites and the epistatic effects of various mutations on e...