A strategy to enhance the insecticidal potency of Vip3Aa by introducing additional cleavage sites to increase its proteolytic activation efficiency [0.03%]
通过引入额外的切割位点提高其蛋白酶活化效率从而增强Vip3Aa杀虫毒力的策略
Kun Jiang,Zhe Chen,Yiting Shi et al.
Kun Jiang et al.
Microbially derived, protein-based biopesticides have become a vital element in pest management strategies. Vip3 family proteins from Bacillus thuringiensis have distinct characteristics from known insecticidal Cry toxins and show efficient...
Identification of multiple regulatory genes involved in TGase production in Streptomyces mobaraensis DSM 40587 [0.03%]
参与Streptomyces mobaraensis DSM 40587TG酶产生的多个调控基因的鉴定
Xian Liu,Dan Wang,Yuru Zhang et al.
Xian Liu et al.
Microbial transglutaminase (TGase) is a protein that is secreted in a mature form and finds wide applications in meat products, tissue scaffold crosslinking, and textile engineering. Streptomyces mobaraensis is the only licensed producer of...
Advances in the dynamic control of metabolic pathways in Saccharomyces cerevisiae [0.03%]
酿酒酵母代谢途径动态调控研究进展
Chufan Xiao,Yuyang Pan,Mingtao Huang
Chufan Xiao
The metabolic engineering of Saccharomyces cerevisiae has great potential for enhancing the production of high-value chemicals and recombinant proteins. Recent studies have demonstrated the effectiveness of dynamic regulation as a strategy ...
Paris I Brown,Adaobi Ojiakor,Antonio J Chemello et al.
Paris I Brown et al.
AB5-type toxins are a group of secreted protein toxins that are central virulence factors for bacterial pathogens such as Shigella dysenteriae, Vibrio cholerae, Bordetella pertussis, and certain lineages of pathogenic Escherichia coli and S...
A study of the protein-protein interactions in the phycocyanin monomer from Synechocystis sp . PCC 6803 using a bacterial two-hybrid system [0.03%]
利用细菌双杂交系统研究热激鱼腥藻藻蓝蛋白单体的蛋白质间相互作用
Simi Shao,Longsheng Zhao,Pingyi Li et al.
Simi Shao et al.
Investigations into the intramolecular interactions of the native protein in solution are important to understand its structural stability as well as its potential uses in future applications. In this study, we used a bacterial two-hybrid s...
Feifei Chen,Jianhe Xu,Gaowei Zheng
Feifei Chen
A multifunctional biocatalyst EneIRED capable of catalyzing amine-activated conjugate alkene reduction and subsequent reductive amination was discovered. The enzyme realized the coupling of α, β-unsaturated carbonyls with amines to effici...
Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products [0.03%]
代谢工程改造链霉菌以增强珍贵天然产物的合成
Zuwei Xu,Lihao Ji,Wenxiu Tang et al.
Zuwei Xu et al.
The mycelial bacterium Streptomyces is a workhorse for producing natural products, serving as a key source of drugs and other valuable chemicals. However, its complicated life cycle, silent biosynthetic gene clusters (BGCs), and poorly char...
Engineering for life in toxicity: Key to industrializing microbial synthesis of high energy density fuels [0.03%]
毒性条件下的生命工程是工业规模微生物合成高能量密度燃料的关键
Lijuan Liu,Wenzhi Bao,Xiao Men et al.
Lijuan Liu et al.
With the growing demand for air transportation combined with global concerns about environmental issues and the instability and lack of renewability of the oil market, microbial production of high energy density fuels for jets (bio-jet fuel...
Biochemical characterization of a polyethylene terephthalate hydrolase and design of high-throughput screening for its directed evolution [0.03%]
聚对苯二甲酸乙二醇酯水解酶的生化表征及高效筛选体系构建用于其定向进化研究
Xiaotong Wang,Chaoyi Song,Qingsheng Qi et al.
Xiaotong Wang et al.
Polyethylene terephthalate (PET), one of the most widely used plastics in the world, causes serious environmental pollution. Recently, researchers have focused their efforts on enzymatic degradation of PET, which is an attractive way of deg...
Yan Zhu,Jinxin Zhao,Jian Li
Yan Zhu
The increasing antimicrobial resistance has seriously threatened human health worldwide over the last three decades. This severe medical crisis and the dwindling antibiotic discovery pipeline require the development of novel antimicrobial t...