Wensheng Qin,Guodong Liu
Wensheng Qin
Jianzhong Lin,Yunxue Guo,Jianyun Yao et al.
Jianzhong Lin et al.
Toxin-antitoxin (TA) systems are ubiquitous in bacteria and archaea. Most are composed of two neighboring genetic elements, a stable toxin capable of inhibiting crucial cellular processes, including replication, transcription, translation, ...
Genetic manipulation and tools in myxobacteria for the exploitation of secondary metabolism [0.03%]
操纵子工具在黏细菌中用于开发次级代谢产物
Xinjing Yue,Duohong Sheng,Li Zhuo et al.
Xinjing Yue et al.
Myxobacteria are famous for their capacity for social behavior and natural product biosynthesis. The unique sociality of myxobacteria is not only an intriguing scientific topic but also the main limiting factor for their manipulation. After...
A highly selective cell-based fluorescent biosensor for genistein detection [0.03%]
一种用于检测染料木黄酮的高选择性细胞荧光生物传感器
Lucy Fang-I Chao,Dany Liu,Verena Siewers
Lucy Fang-I Chao
Genistein, an isoflavone found mainly in legumes, has been shown to have numerous health benefits for humans. Therefore, there is substantial interest in producing it using microbial cell factories. To aid in screening for high genistein pr...
High-level production of γ-aminobutyric acid via efficient co-expression of the key genes of glutamate decarboxylase system in Escherichia coli [0.03%]
通过谷氨酸脱羧酶系统关键基因高效共表达在大肠杆菌中高水平生产γ-氨基丁酸
Lili Yao,Changjiang Lyu,Yuting Wang et al.
Lili Yao et al.
Biosynthesis of the functional factor γ-aminobutyric acid (GABA) in bacteria involves two key proteins an intracellular glutamate decarboxylase (GadB) and a membrane-bound antiporter (GadC). Efficient co-expression of suitable GadB and Gad...
CRISPRi screen highlights chromatin regulation to be involved in formic acid tolerance in Saccharomyces cerevisiae [0.03%]
基于CRISPR干扰的筛选揭示了染色质调控参与酿酒酵母耐甲酸性
Vaskar Mukherjee,Ibai Lenitz,Ulrika Lind et al.
Vaskar Mukherjee et al.
Formic acid is one of the main weak acids in lignocellulosic hydrolysates that is known to be inhibitory to yeast growth even at low concentrations. In this study, we employed a CRISPR interference (CRISPRi) strain library comprising >9000 ...
Hang Li
Hang Li
Biosynthetic pathways without any identifiable core enzymes may encode unknown (biosynthetic route)-unknown (molecular structure) natural products. However, bioinformatics-guided mining for such unknown-unknown metabolites is challenging. R...
Global regulation of fungal secondary metabolism in Trichoderma reesei by the transcription factor Ypr1, as revealed by transcriptome analysis [0.03%]
转录因子Ypr1通过调控网络在曲霉稻属菌中调节次生代谢的研究
Jie Yang,Jia-Xiang Li,Fei Zhang et al.
Jie Yang et al.
Trichoderma reesei Rut-C-30 is a well-known robust producer of cellulolytic enzymes, which are used to degrade lignocellulosic biomass for the sustainable production of biofuels and biochemicals. However, studies of its secondary metabolism...
Prospects for engineering Ralstonia eutropha and Zymomonas mobilis for the autotrophic production of 2,3-butanediol from CO2 and H2 [0.03%]
利用Ralstonia eutropha和Zymomonas mobilis以二氧化碳和氢气为原料合成2,3-丁二醇的前景
Hui Wei,Wei Wang,Yat-Chen Chou et al.
Hui Wei et al.
The decarbonization of the chemical industry and a shift toward circular economies because of high global CO2 emissions make CO2 an attractive feedstock for manufacturing chemicals. Moreover, H2 is a low-cost and carbon-free reductant becau...
Carolina E Portero,Yong Han,M Raquel Marchán-Rivadeneira
Carolina E Portero
Numerous studies have investigated the biosynthesis of pyridine heterocycles derived from nicotinic acid. However, metabolic pathways generating pyridine heterocycles in nature remain uninvestigated. Here, we summarize recent contributions ...