Ruijuan Li,Aiying Li,Youming Zhang et al.
Ruijuan Li et al.
Recombineering is a valuable technique for generating recombinant DNA in vivo, primarily in bacterial cells, and is based on homologous recombination using phage-encoded homologous recombinases, such as Redαβγ from the lambda phage and R...
Profiling proteomic responses to hexokinase-II depletion in terpene-producing Saccharomyces cerevisiae [0.03%]
反式牻牛儿基焦磷酸生产的酵母蛋白组学响应(hexokinase II耗尽)分析
Zeyu Lu,Qianyi Shen,Lian Liu et al.
Zeyu Lu et al.
Hexokinase II (Hxk2) is a master protein in glucose-mediated transcriptional repression signaling pathway. Degrading Hxk2 through an auxin-inducible protein degradation previously doubled sesquiterpene (nerolidol) production at gram-per-lit...
[This corrects the article DOI: 10.1016/j.engmic.2022.100036.][This corrects the article DOI: 10.1016/j.engmic.2022.100020.][This corrects the article DOI: 10.1016/j.engmic.2022.100039.][This corrects the article DOI: 10.1016/j.engmic.2023....
Published Erratum
Engineering microbiology. 2023 Nov 16;3(4):100125. DOI:10.1016/j.engmic.2023.100125 2023
Investigating the cellular functions of β-Glucosidases for synthesis of lignocellulose-degrading enzymes in Trichoderma reesei [0.03%]
探明β-葡萄糖苷酶的细胞功能以在解酯丝孢菌中合成木质纤维素降解酶
Ai-Ping Pang,Haiyan Wang,Yongsheng Luo et al.
Ai-Ping Pang et al.
β-glucosidases play an important role in the synthesis of cellulase in fungi, but their molecular functions and mechanisms remain unknown. We found that the 10 putative β-glucosidases investigated in Trichoderma reesei facilitate cellulas...
CRISPR-based gene editing technology and its application in microbial engineering [0.03%]
基于CRISPR的基因编辑技术及其在微生物工程中的应用
Junwei Wei,Yingjun Li
Junwei Wei
Gene editing technology involves the modification of a specific target gene to obtain a new function or phenotype. Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-mediated technologies have provided...
Xiaoyan Wu,Peng Cai,Lun Yao et al.
Xiaoyan Wu et al.
The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins. Recently, it has gained attention for its potential in producing chemicals from inexpe...
Yun Chen,Jiazhang Lian,Jin Hou
Yun Chen
Combinatorial metabolic engineering of Saccharomyces cerevisiae for improved production of 7-dehydrocholesterol [0.03%]
通过组合代谢工程改善酿酒酵母合成7-脱氢胆固醇水平
Yuehao Gu,Shuhui Chen,Xue Jiao et al.
Yuehao Gu et al.
7-Dehydrocholesterol (7-DHC), a key pharmaceutical intermediate in the production of vitamin D3, has a wide range of applications. To explore fermentative synthesis of 7-DHC, a 7-DHC-producing Saccharomyces cerevisiae strain was constructed...
Yeast surface display of leech hyaluronidase for the industrial production of hyaluronic acid oligosaccharides [0.03%]
利用水蛭透明质酸酶酵母展示技术进行工业生产透明质酸寡糖
Lizhi Liao,Hao Huang,Yang Wang et al.
Lizhi Liao et al.
Leech hyaluronidase (LHyal) is a hyperactive hyaluronic acid (HA) hydrolase that belongs to the hyaluronoglucuronidase family. Traditionally, LHyal is extracted from the heads of leeches, but the recent development of the Pichia pastoris re...
The influence of the copy number of invader on the fate of bacterial host cells in the antiviral defense by CRISPR-Cas10 DNases [0.03%]
侵袭者拷贝数对CRISPR-Cas10 DNases抗病毒防御中细菌宿主细胞命运的影响
Zhenxiao Yu,Jianan Xu,Yan Zhang et al.
Zhenxiao Yu et al.
Type III CRISPR-Cas10 systems employ multiple immune activities to defend their hosts against invasion from mobile genetic elements (MGEs), including DNase and cyclic oligoadenylates (cOA) synthesis both of which are hosted by the type-spec...