Genetically encoded biosensors enabled high-throughput screening of microbial cell factories [0.03%]
基于基因编码的生物传感器实现微生物细胞工厂的高通量筛选
Jin Wang,Xueyan Liu,Longqian Zhao et al.
Jin Wang et al.
Genetically encoded biosensors provide powerful tools for coupling desired phenotypes to detectable outputs and have been extensively developed to detect a wide range of natural and unnatural products. When integrated with diverse high-thro...
Phase variation in Bacteroides fragilis governs susceptibility to a microvirus and drives its evolution [0.03%]
脆弱拟杆菌的相变异影响其对微小病毒的易感性并驱动其进化
Pan Huang,Meiqi Du,Yanqiu Liu et al.
Pan Huang et al.
The interaction and co-evolution between human gut bacteria and their phages shape the dynamic gut microbiome, exerting a significant impact on human health. However, the underlying mechanisms are largely unexplored. In particular, a bacter...
Heterologous expression of Vibrio natriegens transporters enhances the growth of Escherichia coli and Bacillus subtilis on glutamate [0.03%]
异源表达副盐伏尔密菌转运蛋白可增强大肠杆菌和枯草芽孢杆菌利用谷氨酸生长的能力
Fei Han,Qian Wang,Yiheng Wang et al.
Fei Han et al.
Bacterial growth modulation is crucial in microbial synthetic biology. In this study, we found that glutamate is an extremely poor carbon source for Escherichia coli and Bacillus subtilis. The slow growth on glutamate can be effectively ove...
Modulation of Hachiman defence by a type II toxin-antitoxin system via balancing trade-off between the fitness cost and antiphage activity [0.03%]
II型毒素-抗毒素系统通过平衡适应性成本和抗菌活性的权衡来调节Hachiman防御体系
Xuhui Tian,Ruyi Zheng,Xin Li et al.
Xuhui Tian et al.
Hachiman systems provide innate antiphage immunity across prokaryotic domains. The system encodes a HamA nuclease and a HamB helicase both of which exhibit great diversity in sequence. Phylogenetic analyses of HamA and HamB proteins reveale...
Gdx mediates low-affinity Cs⁺/H⁺ antiport and confers cesium resistance in Escherichia coli [0.03%]
Gdx介导低亲和力的Cs⁺/H⁺反向转运并赋予大肠杆菌铯耐受性
Daiki Kojima,Masahiro Ito
Daiki Kojima
Following the Fukushima Daiichi nuclear power plant accident, radioactive cesium was released into the environment, prompting intensified efforts to identify cesium-resistant microorganisms. During these studies, we isolated a cesium-resist...
Analysis of the PRE configuration reveals the constraints in Ste12 oligomerization in mediating pheromone-inducible transcription in Saccharomyces cerevisiae [0.03%]
PRE构型分析揭示了在酿酒酵母中介导激素诱导转录的Ste12低聚化的限制条件
Yiqing Zhang,Yinfeng Wei,Yuxin Huang et al.
Yiqing Zhang et al.
Yeast cell mating is regulated by the transcription factor Ste12, which activates the transcription of mating genes by binding to pheromone response elements (PREs) in their promoters. PREs vary in number and position among different promot...
Advances in polyethylene biodegradation and bioconversion: Microbial, enzymatic, and biotechnological insights [0.03%]
聚乙烯生物降解和生物转化研究进展：微生物、酶和生物技术见解
Jie Qiao,Luxuan Wu,Xiaoru Ma et al.
Jie Qiao et al.
Polyethylene (PE) is one of the most widely used plastics worldwide and is valued for its versatility, durability, and cost-effectiveness. However, the chemical stability of PE combined with its widespread use makes it a persistent environm...
Xiaoying Bian
Xiaoying Bian
The mainstream strategy of genome mining relies on the homologous activation and heterologous expression of target biosynthetic gene clusters (BGCs). However, the efficiency of the current techniques available for new compound discovery har...
Complexity of antibiotic resistance and its impact on gut microbiota dynamics [0.03%]
抗生素抗性的复杂性及其对抗肠道微生物群动态的影响
H Shayista,M N Nagendra Prasad,S Niranjan Raj et al.
H Shayista et al.
The present review explores the influence of the gut microbiota on antibiotic resistance dynamics, particularly those associated with dysbiosis. The improper use of antibiotics can induce resistance in pathogens through various pathways, wh...
BTG13-related metalloenzymes: Atypical non-heme iron-dependent dioxygenases with unusual coordination patterns and catalytic mechanisms [0.03%]
BTG13相关金属酶：具有独特配位模式和催化机制的非血红素铁依赖型二氧 化酶类蛋白质
Zhiwei Deng,Zhenbo Yuan,Zhengshan Luo et al.
Zhiwei Deng et al.
Owing to their diverse coordination patterns and catalytic mechanisms, non-heme iron-dependent dioxygenases catalyze a variety of biochemical reactions involved in the synthesis of numerous natural products and valuable compounds. Recently,...