Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis [0.03%]
五味子 lactone生物合成中细胞色素P450催化的戊烯炔醇氧化为1-脱氧戊烯酸𬭩离子𬭩离子
Jing Li,Chengde Zhang,Shiwen Wu et al.
Jing Li et al.
Pentalenolactone is a sesquiterpene antibiotic from Streptomyces. Its biosynthetic pathway has been elucidated, except for the oxidation of pentalen-13-al to 1-deoxypentalenic acid. In this study, we show that cytochrome P450 pentalenene ox...
Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica [0.03%]
优化CRISPR / Cas9系统以用于安氏志贺氏菌基因编辑
Jianhui Liu,Yamin Zhu,Jin Hou
Jianhui Liu
Yarrowia lipolytica is a promising host for producing valuable chemicals owing to its robustness and metabolic versatility. Efficient genome editing tools are essential for advancing its biotechnological applications. Although CRISPR/Cas9 t...
Carbon sequestration pathways in microorganisms: Advances, strategies, and applications [0.03%]
微生物固碳途径：进展、策略与应用
Shupeng Ruan,Yuchen Jiang,Aoxue Wang et al.
Shupeng Ruan et al.
In recent years, industrial activities have significantly increased atmospheric CO2 levels, exacerbating global warming. Carbon reduction involves implementing measures to minimize CO2 emissions from human activities and achieve a balance b...
Dongchun Ni
Dongchun Ni
Bacteria employ diverse immune systems, such as CRISPR-Cas, to fend off phage infections. A recent study uncovered the unprecedented mechanistic features of the Kongming bacterial defense system, which uniquely exploits phage-derived enzyme...
Superoxide-mediated O2 activation drives radical cyclization in ergot alkaloid biosynthesis [0.03%]
超氧合介导的O2活化驱动了麦角生物碱生物合成中的自由基环化反应
Yuanyuan Jiang,Zhong Li,Shengying Li
Yuanyuan Jiang
Conventional heme enzymes utilize iron-oxygen intermediates to activate substrates and drive reactions. Recently, Chen et al. discovered a novel NADPH-independent superoxide mechanism of heme catalase EasC, which facilitates an O2-dependent...
Functional heterologous expression of the reversible Cu-decarboxylase from the lichen, Cladonia uncialis [0.03%]
来自地钱Cladonia uncialis的可逆Cu-去羧酶的功能异源表达
Harman Gill,John L Sorensen
Harman Gill
Despite the isolation of over 1000 known bioactive lichen mycobiont-derived secondary metabolites (SMs), understanding the genetic basis of their biosynthesis remains elusive. Biosynthetic gene clusters (BGCs) have been tentatively linked t...
Advances in synthetic microbial ecosystems approach for studying ecological interactions and their influencing factors [0.03%]
合成微生物生态系统研究进展：生态互作及驱动因素
Wei Jiang,Sumeng Wang,Fei Gu et al.
Wei Jiang et al.
Investigating ecological interactions within microbial ecosystems is essential for enhancing our comprehension of key ecological issues, such as community stability, keystone species identification, and the manipulation of community structu...
Characterization of Bacillus subtilis phages PJNB030, PJNB031, and PJNB032 reveals wall teichoic acid as a key receptor determinant [0.03%]
Bacillus subtilis噬菌体PJNB030、PJNB031和PJNB032的表征揭示了细胞壁-teichoic酸作为关键受体决定因素
Jiaoyang Song,Ming Hu,Min Zhao et al.
Jiaoyang Song et al.
Bacillus subtilis is widely used in industrial fermentation and probiotic applications; however, phage contamination poses a substantial economic threat. To address this, we isolated three phages (PJNB030, PJNB031, and PJNB032) from a conta...
Effect of resistant starch type 5 on gut health through modulating gut microbiota [0.03%]
抗性淀粉5型通过调节肠道微生物群对肠道健康的影响
Raju Ahmmed,Andrew Paff,Lingyan Kong et al.
Raju Ahmmed et al.
Resistant starch is a dietary fiber that escapes digestion in the small intestine and undergoes fermentation by gut microbiota in the colon, producing beneficial short-chain fatty acids (SCFAs). Among the various types of resistant starch, ...
Advances in microbial mevalonolactone production: from fermentative mevalonate accumulation to downstream lactonization [0.03%]
微生物甲基赤藓糖醇焦磷酸代谢途径研究进展：发酵生产发酵积累mekv及下游环化技术改造
Hao Tang,Lihong Yin,Yiwen Jiang et al.
Hao Tang et al.
Mevalonolactone (MVAL) is a high-value feedstock for the cosmetic industry, with (R)-(-)-MVAL as the sole bioactive enantiomer. Chemical synthesis, which is the traditional method for MVAL production, is hindered by cumbersome procedures, l...