Aquaculture sustainability through alternative dietary ingredients: Microalgal value-added products [0.03%]
微藻高价值产品在水产养殖可持续性中的作用：替代饲料原料
John N Idenyi,Jonathan C Eya,Amechi S Nwankwegu et al.
John N Idenyi et al.
Aquaculture contributes remarkably to the global economy and food security through seafood production, an important part of the global food supply chain. The success of this industry depends heavily on aquafeeds, and the nutritional composi...
Lysine acetylation decreases enzyme activity and protein level of Escherichia coli lactate dehydrogenase [0.03%]
丝氨酸乙酰化降低大肠杆菌乳酸脱氢酶的活性和蛋白水平
Min Liu,Meitong Huo,Likun Guo et al.
Min Liu et al.
Lactate is an important bulk chemical with widespread applications and a major byproduct of other chemicals bioprocess in microbial fermentation. Lactate dehydrogenase A (LdhA) catalyzes the synthesis of lactate from pyruvate. Lysine acetyl...
Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomona s using phage-encoded homologous recombination [0.03%]
利用噬菌体编码同源重组在假单胞门中进行高精度基因组编辑的Cascade-Cas3作用机制研究
Wentao Zheng,Yandong Xia,Xue Wang et al.
Wentao Zheng et al.
Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a Pseudomonas-specific PEHR system. However, when using the PEHR system for Pseudomonas genome editing, fa...
Jianping Huang,Shengxiong Huang
Jianping Huang
The de novo biosynthesis of vindoline and catharanthine, the direct precursors used for industrial production of the anti-cancer drug vinblastine, has been achieved in the yeast cell factory. To date, this is the longest natural product bio...
A chemoenzymatic cascade with the potential to feed the world and allow humans to live in space [0.03%]
一种有潜力解决世界粮食问题并允许人类在太空生活的方法
Shuke Wu,Uwe T Bornscheuer
Shuke Wu
While the typical targets of (chemo-)enzymatic cascades are fine chemicals (e.g., pharmaceuticals), a chemoenzymatic cascade, artificial starch anabolic pathway (ASAP), was recently developed to synthesize starch from CO2. The key results a...
In vitro characterization of a nitro-forming oxygenase involved in 3-(trans-2'-aminocyclopropyl)alanine biosynthesis [0.03%]
参与3-(反式-2'-氨基环丙基)甘氨酸生物合成的硝基形成氧合酶的体外表征
Linlin Pang,Weijing Niu,Yuwei Duan et al.
Linlin Pang et al.
In vitro characterization experiments revealed the formations of 3-(trans-2'-aminocyclopropyl)alanine ((3-Acp)Ala) and 3-(trans-2'-nitrocyclopropyl)alanine ((3-Ncp)Ala) are originated via two homologous proteins, BelK and HrmI, which regios...
Shengling Xie,Lihan Zhang
Shengling Xie
The redox cofactor β-nicotinamide adenine dinucleotide (NAD) has been revealed to serve as a building block for the biosynthesis of an alkaloid natural product, altemicidin. The biosynthetic pathway investigation identified a unique pyrido...
A comparative study on the genomes, transcriptomes, and metabolic properties of Escherichia coli strains Nissle 1917, BL21(DE3), and MG1655 [0.03%]
利用比较基因组学和转录组学研究大肠杆菌Nissle 1917、BL21（DE3）和MG1655菌株的基因功能及代谢特性差异
Linlin Zhao,Guobin Yin,Yonglin Zhang et al.
Linlin Zhao et al.
Escherichia coli is the most well-studied model prokaryote and has become an indispensable host for the biotechnological production of proteins and biochemicals. In particular, the probiotic status of one E. coli strain, E. coli Nissle 1917...
Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp. PCC 6803 Δ hoxYH [0.03%]
变异体 Synechocystis sp. PCC 6803 Δ hoxYH 中异源羟基异己酸脱氢酶的表达和活性分析
Valentina Jurkaš,Christoph K Winkler,Silvan Poschenrieder et al.
Valentina Jurkaš et al.
Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of energy. Consequently, cyanobacteria, which can use light e.g., for generating NADPH, are in the focus of r...
Functional expression and regulation of eukaryotic cytochrome P450 enzymes in surrogate microbial cell factories [0.03%]
真核生物细胞色素P450酶在微生物细胞工厂中的功能表达和调控
Pradeepraj Durairaj,Shengying Li
Pradeepraj Durairaj
Cytochrome P450 (CYP) enzymes play crucial roles during the evolution and diversification of ancestral monocellular eukaryotes into multicellular eukaryotic organisms due to their essential functionalities including catalysis of housekeepin...