Genome-scale CRISPRi screening: A powerful tool in engineering microbiology [0.03%]
基因组规模的CRISPRi筛选技术：工程微生物学中的有力工具
Letian Sun,Ping Zheng,Jibin Sun et al.
Letian Sun et al.
Deciphering gene function is fundamental to engineering of microbiology. The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adapted for gene repression across a range of hosts, creating a versatile tool c...
A recombineering system for Bacillus subtilis based on the native phage recombinase pair YqaJ/YqaK [0.03%]
基于固有噬菌体重组酶对YqaJ/YqaK的芽孢杆菌bsubtilis重组工程系统
Qingshu Liu,Ruijuan Li,Hongbo Shi et al.
Qingshu Liu et al.
Bacillus subtilis plays an important role in fundamental and applied research, and it has been widely used as a cell factory for the production of enzymes, antimicrobial materials, and chemicals for agriculture, medicine, and industry. Howe...
Engineering of Saccharomyces cerevisiae for co-fermentation of glucose and xylose: Current state and perspectives [0.03%]
用于葡萄糖和木糖联合发酵的酿酒酵母工程化研究：现状与展望
Yali Qiu,Meiling Wu,Haodong Bao et al.
Yali Qiu et al.
The use of non-food lignocellulosic biomass to produce ethanol fits into the strategy of a global circular economy with low dependence on fossil energy resources. Xylose is the second most abundant sugar in lignocellulosic hydrolysate, and ...
Recombineering enables genome mining of novel siderophores in a non-model Burkholderiales strain [0.03%]
利用基因组重建技术在非模式菌株Burkholderiales中挖掘新型铁载体
Xingyan Wang,Haibo Zhou,Xiangmei Ren et al.
Xingyan Wang et al.
Iron is essential for bacterial survival, and most bacteria capture iron by producing siderophores. Burkholderiales bacteria produce various types of bioactive secondary metabolites, such as ornibactin and malleobactin siderophores. In this...
Catalog of operational taxonomic units and unified amplicon sequencing data for the microbiomes of medicinal plant roots [0.03%]
中药植物根系微生物组的操作分类单元目录和统一扩增子测序数据
Meng Wang,Ming Lei,Hailun He
Meng Wang
China has a rich history of cultivating medicinal plants, whose root microbial communities closely interact with the medicinal plants, thereby influencing their growth, health, and medicinal properties. Currently, researchers widely use 16S...
Recent advances in the direct cloning of large natural product biosynthetic gene clusters [0.03%]
天然产物生物合成基因簇的直接克隆研究进展
Jiaying Wan,Nan Ma,Hua Yuan
Jiaying Wan
Large-scale genome-mining analyses have revealed that microbes potentially harbor a huge reservoir of uncharacterized natural product (NP) biosynthetic gene clusters (BGCs), and this has spurred a renaissance of novel drug discovery. Howeve...
The lasso structure, biosynthesis, bioactivities and potential applications of Microcin J25: A novel antibacterial agent with unique mechanisms [0.03%]
新型抗菌剂微链J25的结构、合成、活性及应用潜力研究进展
Qingchun Ji,Bixia Zhou,Tong Shen et al.
Qingchun Ji et al.
The overuse and misuse of traditional antimicrobial drugs have led to their weakened effectiveness and the emergence of pathogenic bacterial resistance. Consequently, there has been growing interest in alternative options such as antimicrob...
Ruijuan Li,Aiying Li,Youming Zhang et al.
Ruijuan Li et al.
Recombineering is a valuable technique for generating recombinant DNA in vivo, primarily in bacterial cells, and is based on homologous recombination using phage-encoded homologous recombinases, such as Redαβγ from the lambda phage and R...
Profiling proteomic responses to hexokinase-II depletion in terpene-producing Saccharomyces cerevisiae [0.03%]
反式牻牛儿基焦磷酸生产的酵母蛋白组学响应(hexokinase II耗尽)分析
Zeyu Lu,Qianyi Shen,Lian Liu et al.
Zeyu Lu et al.
Hexokinase II (Hxk2) is a master protein in glucose-mediated transcriptional repression signaling pathway. Degrading Hxk2 through an auxin-inducible protein degradation previously doubled sesquiterpene (nerolidol) production at gram-per-lit...
[This corrects the article DOI: 10.1016/j.engmic.2022.100036.][This corrects the article DOI: 10.1016/j.engmic.2022.100020.][This corrects the article DOI: 10.1016/j.engmic.2022.100039.][This corrects the article DOI: 10.1016/j.engmic.2023....
Published Erratum
Engineering microbiology. 2023 Nov 16;3(4):100125. DOI:10.1016/j.engmic.2023.100125 2023