A mouse model for the study of anti-tumor T cell responses in Kras-driven lung adenocarcinoma [0.03%]
Kras驱动的肺腺癌中研究抗肿瘤T细胞反应的小鼠模型
Brittany Fitzgerald,Kelli A Connolly,Can Cui et al.
Brittany Fitzgerald et al.
Kras-driven lung adenocarcinoma (LUAD) is the most common lung cancer. A significant fraction of patients with Kras-driven LUAD respond to immunotherapy, but mechanistic studies of immune responses against LUAD have been limited because of ...
Robert A Policastro,Gabriel E Zentner
Robert A Policastro
Transcription start site (TSS) selection influences transcript stability and translation as well as protein sequence. Alternative TSS usage is pervasive in organismal development, is a major contributor to transcript isoform diversity in hu...
A probabilistic framework for cellular lineage reconstruction using integrated single-cell 5-hydroxymethylcytosine and genomic DNA sequencing [0.03%]
结合单细胞5-羟甲基胞嘧啶和基因组DNA测序的细胞系重构概率框架
Chatarin Wangsanuwat,Alex Chialastri,Javier F Aldeguer et al.
Chatarin Wangsanuwat et al.
Lineage reconstruction is central to understanding tissue development and maintenance. To overcome the limitations of current techniques that typically reconstruct clonal trees using genetically encoded reporters, we report scPECLR, a proba...
Development of a pan-neuronal genetic driver in Aedes aegypti mosquitoes [0.03%]
在埃及伊蚊中开发一个泛神经元基因驱动系统
Zhilei Zhao,David Tian,Carolyn S McBride
Zhilei Zhao
The recent development of neurogenetic tools in Aedes aegypti mosquitoes is beginning to shed light on the neural basis of behaviors that make this species a major vector of human disease. However, we still lack a pan-neuronal expression dr...
A method for characterizing daily physiology from widely used wearables [0.03%]
一种通过常用的可穿戴设备来描述日常生理状态的方法
Clark Bowman,Yitong Huang,Olivia J Walch et al.
Clark Bowman et al.
Millions of wearable-device users record their heart rate (HR) and activity. We introduce a statistical method to extract and track six key physiological parameters from these data, including an underlying circadian rhythm in HR (CRHR), the...
Highly efficient manipulation of nervous system gene expression with NEPTUNE [0.03%]
利用NEPTUNE高效操控神经系统基因表达
Katrin Mangold,Jan Mašek,Jingyan He et al.
Katrin Mangold et al.
Genetic loss and gain of function in mice have typically been studied by using knockout or knockin mice that take months to years to generate. To address this problem for the nervous system, we developed NEPTUNE (NEural Plate Targeting by i...
Uncovering biomarkers during therapeutic neuromodulation with PARRM: Period-based Artifact Reconstruction and Removal Method [0.03%]
在治疗性神经调节过程中使用PARRM发现生物标志物：基于时期的 artifact 重构和移除方法
Evan M Dastin-van Rijn,Nicole R Provenza,Jonathan S Calvert et al.
Evan M Dastin-van Rijn et al.
Advances in therapeutic neuromodulation devices have enabled concurrent stimulation and electrophysiology in the central nervous system. However, stimulation artifacts often obscure the sensed underlying neural activity. Here, we develop a ...
Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms [0.03%]
跨多重组织成像平台实现可重复、可扩展和稳健的数据分析
Erik A Burlingame,Jennifer Eng,Guillaume Thibault et al.
Erik A Burlingame et al.
The emergence of megascale single-cell multiplex tissue imaging (MTI) datasets necessitates reproducible, scalable, and robust tools for cell phenotyping and spatial analysis. We developed open-source, graphics processing unit (GPU)-acceler...
A simple and robust method for simultaneous dual-omics profiling with limited numbers of cells [0.03%]
一种使用有限数量的细胞进行同时双组学谱分析的简单且稳健的方法
Ruifang Li,Sara A Grimm,Paul A Wade
Ruifang Li
Deciphering epigenetic regulation of gene expression requires measuring the epigenome and transcriptome jointly. Single-cell multi-omics technologies have been developed for concurrent profiling of chromatin accessibility and gene expressio...
A size-exclusion-based approach for purifying extracellular vesicles from human plasma [0.03%]
一种基于尺寸排除的从人血浆中提纯细胞外囊泡的方法
Patrick M Vanderboom,Surendra Dasari,Gregory N Ruegsegger et al.
Patrick M Vanderboom et al.
Extracellular vesicles (EVs) are released into blood from multiple organs and carry molecular cargo that facilitates inter-organ communication and an integrated response to physiological and pathological stimuli. Interrogation of the protei...