Distinct classes of potassium channels fused to GPCRs as electrical signaling biosensors [0.03%]
用于电生理传感器的钾离子通道与G蛋白偶联受体的融合及不同的钾通道类别
M Dolores García-Fernández,Franck C Chatelain,Hugues Nury et al.
M Dolores García-Fernández et al.
Ligand-gated ion channels (LGICs) are natural biosensors generating electrical signals in response to the binding of specific ligands. Creating de novo LGICs for biosensing applications is technically challenging. We have previously designe...
Transcriptome signature of cell viability predicts drug response and drug interaction in Mycobacterium tuberculosis [0.03%]
结核分枝杆菌中转录特征对细胞存活性的预测决定药物反应和相互作用
Vivek Srinivas,Rene A Ruiz,Min Pan et al.
Vivek Srinivas et al.
There is an urgent need for new drug regimens to rapidly cure tuberculosis. Here, we report the development of drug response assayer (DRonA) and "MLSynergy," algorithms to perform rapid drug response assays and predict response of Mycobacte...
A fast, aqueous, reversible three-day tissue clearing method for adult and embryonic mouse brain and whole body [0.03%]
一种快速的、可逆的水溶性三维脑组织光学清除方法及其应用
Stylianos Kosmidis,Adrian Negrean,Alex Dranovsky et al.
Stylianos Kosmidis et al.
Optical clearing methods serve as powerful tools to study intact organs and neuronal circuits. We developed an aqueous clearing protocol, Fast 3D Clear, that relies on tetrahydrofuran for tissue delipidation and iohexol for clearing, such t...
An optimized genome-wide, virus-free CRISPR screen for mammalian cells [0.03%]
适用于哺乳动物细胞的优化型无病毒全基因组CRISPR筛选体系
Kai Xiong,Karen Julie la Cour Karottki,Hooman Hefzi et al.
Kai Xiong et al.
Pooled CRISPR screens have been widely applied to mammalian and other organisms to elucidate the interplay between genes and phenotypes of interest. The most popular method for delivering the CRISPR components into mammalian cells is lentiv...
Genetically encoded biosensors for evaluating NAD+/NADH ratio in cytosolic and mitochondrial compartments [0.03%]
用于评估细胞质和线粒体区室中NAD + / NADH比率的基因编码生物传感器
Qingxun Hu,Dan Wu,Matthew Walker et al.
Qingxun Hu et al.
The ratio of oxidized to reduced NAD (NAD+/NADH) sets intracellular redox balance and antioxidant capacity. Intracellular NAD is compartmentalized and the mitochondrial NAD+/NADH ratio is intricately linked to cellular function. Here, we re...
Uncovering spatial representations from spatiotemporal patterns of rodent hippocampal field potentials [0.03%]
从实验鼠海马体的场电位的时空模型中发掘空间影像
Liang Cao,Viktor Varga,Zhe S Chen
Liang Cao
Spatiotemporal patterns of large-scale spiking and field potentials of the rodent hippocampus encode spatial representations during maze runs, immobility, and sleep. Here, we show that multisite hippocampal field potential amplitude at ultr...
A deep learning-based segmentation pipeline for profiling cellular morphodynamics using multiple types of live cell microscopy [0.03%]
基于深度学习的细胞形态动力学分析分割管道的研究利用多种类型的活细胞显微镜技术
Junbong Jang,Chuangqi Wang,Xitong Zhang et al.
Junbong Jang et al.
Motivation: Quantitative studies of cellular morphodynamics rely on extracting leading-edge velocity time series based on accurate cell segmentation from live cell imaging. However, live cell imaging has numerous challeng...
In situ functional cell phenotyping reveals microdomain networks in colorectal cancer recurrence [0.03%]
原位功能细胞表型揭示结直肠癌复发中的微域网络
Samantha A Furman,Andrew M Stern,Shikhar Uttam et al.
Samantha A Furman et al.
Tumors are dynamic ecosystems comprising localized niches (microdomains), possessing distinct compositions and spatial configurations of cancer and non-cancer cell populations. Microdomain-specific network signaling coevolves with a continu...
Metatranscriptomics to characterize respiratory virome, microbiome, and host response directly from clinical samples [0.03%]
利用宏转录组学直接从临床样品中表征呼吸病毒组、微生物组和宿主反应
Seesandra V Rajagopala,Nicole G Bakhoum,Suman B Pakala et al.
Seesandra V Rajagopala et al.
We developed a metatranscriptomics method that can simultaneously capture the respiratory virome, microbiome, and host response directly from low biomass samples. Using nasal swab samples, we capture RNA virome with sufficient sequencing de...
Sven T Sowa,Albert Galera-Prat,Sarah Wazir et al.
Sven T Sowa et al.
Proteins interacting with ADP-ribosyl groups are often involved in disease-related pathways or viral infections, making them attractive drug targets. We present a robust and accessible assay applicable to both hydrolyzing or non-hydrolyzing...