Wenpin Hou,Zhicheng Ji
Wenpin Hou
Visualizing low-dimensional representations with scatterplots is a crucial step in analyzing single-cell genomic data. However, this visualization has significant biases. The first bias arises when visualizing the gene expression levels or ...
Karolina Sienkiewicz,Jinyu Chen,Ajay Chatrath et al.
Karolina Sienkiewicz et al.
We present a data integration framework that uses non-negative matrix factorization of patient-similarity networks to integrate continuous multi-omics datasets for molecular subtyping. It is demonstrated to have the capability to handle mis...
Rapid detection of multiple SARS-CoV-2 variants of concern by PAM-targeting mutations [0.03%]
通过PAM位点靶标突变快速检测多种关注变异株
Bo Ning,Brady M Youngquist,Diane D Li et al.
Bo Ning et al.
SARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. Current methods available to rapidly detect these can be resource intensive a...
An intravitreal implant injection method for sustained drug delivery into mouse eyes [0.03%]
一种向小鼠眼睛玻璃体内植入药物递送装置的方法
Young Joo Sun,Cheng-Hui Lin,Man-Ru Wu et al.
Young Joo Sun et al.
Using small molecule drugs to treat eye diseases carries benefits of specificity, scalability, and transportability, but their efficacy is significantly limited by a fast intraocular clearance rate. Ocular drug implants (ODIs) present a com...
Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection [0.03%]
用于研究人冠状病毒RNA的多重彩色超分辨率成像技术
Jiarui Wang,Mengting Han,Anish R Roy et al.
Jiarui Wang et al.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current...
Enrichment of gut microbiome strains for cultivation-free genome sequencing using droplet microfluidics [0.03%]
利用微流控液滴对肠道微生物菌株进行无培养基因组测序的优化
Anna Pryszlak,Tobias Wenzel,Kiley West Seitz et al.
Anna Pryszlak et al.
We report a droplet microfluidic method to target and sort individual cells directly from complex microbiome samples and to prepare these cells for bulk whole-genome sequencing without cultivation. We characterize this approach by recoverin...
Macrophage calcium reporter mice reveal immune cell communication in vitro and in vivo [0.03%]
巨噬细胞钙信号报告小鼠揭示体内外免疫细胞通讯
Nika Taghdiri,David M Calcagno,Zhenxing Fu et al.
Nika Taghdiri et al.
Cell communication underlies emergent functions in diverse cell types and tissues. Recent evidence suggests that macrophages are organized in communicating networks, but new tools are needed to quantitatively characterize the resulting cell...
Sequence-selective purification of biological RNAs using DNA nanoswitches [0.03%]
使用DNA纳米开关选择性纯化生物RNA
Lifeng Zhou,Andrew Hayden,Arun Richard Chandrasekaran et al.
Lifeng Zhou et al.
Nucleic acid purification is a critical aspect of biomedical research and a multibillion-dollar industry. Here we establish sequence-selective RNA capture, release, and isolation using conformationally responsive DNA nanoswitches. We valida...
CUT&Tag-BS for simultaneous profiling of histone modification and DNA methylation with high efficiency and low cost [0.03%]
高效经济的染色质免疫沉淀联合 bisulfite 测序技术同时检测组蛋白修饰和DNA甲基化水平
Ruifang Li,Sara A Grimm,Paul A Wade
Ruifang Li
It remains a challenge to decipher the complex relationship between DNA methylation, histone modification, and the underlying DNA sequence with limited input material. Here, we developed an efficient, low-input, and low-cost method for the ...
Maximizing statistical power to detect differentially abundant cell states with scPOST [0.03%]
利用scPOST检测具有统计功效的细胞状态差异丰度的方法
Nghia Millard,Ilya Korsunsky,Kathryn Weinand et al.
Nghia Millard et al.
To estimate a study design's power to detect differential abundance, we require a framework that simulates many multi-sample single-cell datasets. However, current simulation methods are challenging for large-scale power analyses because th...