Real-time pH imaging of macrophage lysosomes using the pH-sensitive probe ApHID [0.03%]
基于pH敏感探针ApHID的巨噬细胞溶酶体实时pH成像研究
Santiago Solé-Domènech,Pradeep Kumar Singh,Lucy Funes et al.
Santiago Solé-Domènech et al.
Published Erratum
Cell reports methods. 2025 Oct 24:101240. DOI:10.1016/j.crmeth.2025.101240 2025
An optimized click chemistry method allows visualization of proliferating neuronal progenitors in the mouse brain [0.03%]
一种优化的点击化学方法能够使小鼠脑中增殖的神经前体细胞可视化
Fei Zhao,Tomonari Hamaguchi,Ryo Egawa et al.
Fei Zhao et al.
We establish a click reaction-based workflow (tissue clearing coupled with click-chemistry in 3D, C4-3D) to visualize 5-ethynyl-2'-deoxyuridine (EdU) in whole mouse brain tissue cleared by CUBIC, iDisco+, and PACT. C4-3D was compatible with...
Hadi Yassine,Elizabeta Sirotkin,Omer Goldberger et al.
Hadi Yassine et al.
Rapid, spatially controlled methods are needed to investigate RNA localization in bacterial cells. APEX2 proximity labeling was shown to be adaptable to rapid RNA labeling in eukaryotic cells and, through the fusion of APEX2 to different pr...
OLIVE provides rapid visualization and analysis of chromatin tracing experiments [0.03%]
OLIVE实现染色质追踪实验的快速可视化和分析
Yeqiao Zhou,Yimin Sheng,Dongbo Hu et al.
Yeqiao Zhou et al.
Optical chromatin tracing experiments directly capture the three-dimensional folding of thousands of individual alleles, highlighting the need for a tool that enables fast, interactive, and analytical browsing of such data. Here, we introdu...
Deep learning finds convergent melanocytic morphology despite noisy archival slides [0.03%]
深度学习发现了黑色素细胞形态的收敛性，尽管档案载玻片质量参差不齐
Mikio Tada,Garrett Gaskins,Sina Ghandian et al.
Mikio Tada et al.
Melanocytic atypia often leads to diagnostic discordance, complicating its prediction by machine learning models. To overcome this, we paired H&E-stained histology images with contiguous or serial sections immunohistochemically (IHC) staine...
AIstain: Enhancing microglial phagocytosis analysis through deep learning [0.03%]
基于深度学习技术增强小胶质细胞吞噬作用分析AIstain研究
Alexander Zähringer,Janaki Manoja Vinnakota,Tobias Wertheimer et al.
Alexander Zähringer et al.
Investigating microglial phagocytosis is essential for understanding the mechanisms underlying brain health and disease. Dysregulation of phagocytosis is implicated in various neurological disorders, necessitating accurate analysis. Leverag...
stTransfer enables transfer of single-cell annotations to spatial transcriptomics with single-cell resolution [0.03%]
基于单细胞分辨率的stTransfer方法实现单细胞注释到空间转录组数据的转移
Tao Zhou,Lin Xiang,Kuo Liao et al.
Tao Zhou et al.
Spatial transcriptomics (ST) enables in situ analysis of gene expression patterns and spatial microenvironments. However, current ST technologies are limited by detection sensitivity and gene coverage, posing significant challenges for prec...
Using spatial proteomics to enhance cell type assignments in histology images [0.03%]
利用空间蛋白质组学增强组织学图像中细胞类型的分配
Monica T Dayao,Aaron T Mayer,Alexandro E Trevino et al.
Monica T Dayao et al.
Hematoxylin and eosin (H&E) staining has been a standard in clinical histopathology for many decades but lacks molecular detail. Advances in multiplexed spatial proteomics imaging allow cell types and tissues to be annotated by their expres...
Real-time pH imaging of macrophage lysosomes using the pH-sensitive probe ApHID [0.03%]
基于pH敏感探针ApHID的巨噬细胞溶酶体实时pH成像研究
Santiago Solé-Domènech,Pradeep Kumar Singh,Lucy Funes et al.
Santiago Solé-Domènech et al.
Active endolysosomal pH regulation is essential for optimal enzymatic activity. To measure acidification, pH sensors can be delivered to acidic compartments using labeled dextran polymers or proteins. However, commercial probes have limited...
Lucy Liang,Alessandro Fasse,Arianna Damiani et al.
Lucy Liang et al.
High-definition visualization techniques are critical for understanding the neuroanatomy of the spinal cord, an essential structure for sensorimotor and autonomic functions, in both healthy and pathological conditions. Magnetic resonance im...