Single-cell intracellular epitope and transcript detection reveals signal transduction dynamics [0.03%]
单细胞胞内表位和转录检测可揭示信号转导动力学
Francesca Rivello,Erik van Buijtenen,Kinga Matuła et al.
Francesca Rivello et al.
To further our understanding of how biochemical information flows through cells upon external stimulation, we require single-cell multi-omics methods that concurrently map changes in (phospho)protein levels across signaling networks and the...
A mixture-of-experts deep generative model for integrated analysis of single-cell multiomics data [0.03%]
单细胞多组学数据综合分析的专家混合深度生成模型
Kodai Minoura,Ko Abe,Hyunha Nam et al.
Kodai Minoura et al.
The recent development of single-cell multiomics analysis has enabled simultaneous detection of multiple traits at the single-cell level, providing deeper insights into cellular phenotypes and functions in diverse tissues. However, currentl...
Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitment [0.03%]
集基因表达与染色质可及性解析人红细胞谱系决定中的转录因子作用机制
Reema Baskar,Amy F Chen,Patricia Favaro et al.
Reema Baskar et al.
Master transcription factors (TFs) directly regulate present and future cell states by binding DNA regulatory elements and driving gene-expression programs. Their abundance influences epigenetic priming to different cell fates at the chroma...
Karam Khateeb,Julien Bloch,Jasmine Zhou et al.
Karam Khateeb et al.
Lesioning and neurophysiological studies have facilitated the elucidation of cortical functions and mechanisms of functional recovery following injury. Clinical translation of such studies is contingent on their employment in non-human prim...
A kinetic fluorescence polarization ligand assay for monitoring BAX early activation [0.03%]
监测BAX早期激活的荧光偏振配体测定法
Jesse D Gelles,Jarvier N Mohammed,Yiyang Chen et al.
Jesse D Gelles et al.
Developmental, homeostatic, and pharmacological pro-apoptotic signals converge by activating the BCL-2 family member BAX. Studies investigating molecular regulation of BAX are commonly limited to methodologies measuring endpoint phenotypes ...
Jared S Rosenblum,Anthony J Cappadona,Pashayar P Lookian et al.
Jared S Rosenblum et al.
Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire in situ cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualiz...
High-throughput identification of autoantibodies that target the human exoproteome [0.03%]
针对人类外源蛋白的人-auto抗体的高通量识别方法
Eric Y Wang,Yile Dai,Connor E Rosen et al.
Eric Y Wang et al.
Autoantibodies that recognize extracellular proteins (the exoproteome) exert potent biological effects but are challenging to detect. Here, we developed rapid extracellular antigen profiling (REAP), a high-throughput technique for the compr...
Dual data and motif clustering improves the modeling and interpretation of phosphoproteomic data [0.03%]
双重数据和基序聚类提高了磷酸蛋白质组学数据的建模和解释能力
Marc Creixell,Aaron S Meyer
Marc Creixell
Cell signaling is orchestrated in part through a network of protein kinases and phosphatases. Dysregulation of kinase signaling is widespread in diseases such as cancer and is readily targetable through inhibitors. Mass spectrometry-based a...
Homebrew: An economical and sensitive glassmilk-based nucleic-acid extraction method for SARS-CoV-2 diagnostics [0.03%]
家用自酿法：一种经济且敏感的基于玻璃奶的核酸提取方法用于SARS-CoV-2诊断
Robert Page,Edward Scourfield,Mattia Ficarelli et al.
Robert Page et al.
Management of COVID-19 and other epidemics requires large-scale diagnostic testing. The gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains reverse transcription quantitative PCR (qRT-PCR) analys...
Development of a hybrid alphavirus-SARS-CoV-2 pseudovirion for rapid quantification of neutralization antibodies and antiviral drugs [0.03%]
开发一种混合型α病毒-SARS-CoV-2假病毒体系，用于快速检测中和抗体及抗病毒药物效力
Brian Hetrick,Linda D Chilin,Sijia He et al.
Brian Hetrick et al.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S)-pseudotyped viruses are commonly used for quantifying antiviral drugs and neutralizing antibodies. Here, we describe the development of a hybrid alphavirus-SARS-...