Inference of high-resolution trajectories in single-cell RNA-seq data by using RNA velocity [0.03%]
利用RNA速度推断单细胞RNA测序数据中的高分辨率轨迹
Ziqi Zhang,Xiuwei Zhang
Ziqi Zhang
Trajectory inference (TI) methods infer cell developmental trajectory from single-cell RNA sequencing data. Current TI methods can be categorized into those using RNA velocity information and those using only single-cell gene expression dat...
Probe design for simultaneous, targeted capture of diverse metagenomic targets [0.03%]
适用于多种宏基因组目标的同时捕获的探针设计
Zachery W Dickson,Dirk Hackenberger,Melanie Kuch et al.
Zachery W Dickson et al.
The compounding challenges of low signal, high background, and uncertain targets plague many metagenomic sequencing efforts. One solution has been DNA capture, wherein probes are designed to hybridize with target sequences, enriching them i...
Development of mammalian cell logic gates controlled by unnatural amino acids [0.03%]
由人工氨基酸控制的哺乳动物细胞逻辑门的发展
Emily M Mills,Victoria L Barlow,Arwyn T Jones et al.
Emily M Mills et al.
Mammalian cell logic gates hold great potential for wide-ranging applications. However, most of those currently available are controlled by drug(-like) molecules with inherent biological activities. To construct truly orthogonal circuits an...
A weakly supervised deep learning approach for label-free imaging flow-cytometry-based blood diagnostics [0.03%]
一种弱监督深度学习方法，用于无标签的基于流动式细胞仪的血液诊断技术中
Corin F Otesteanu,Martina Ugrinic,Gregor Holzner et al.
Corin F Otesteanu et al.
The application of machine learning approaches to imaging flow cytometry (IFC) data has the potential to transform the diagnosis of hematological diseases. However, the need for manually labeled single-cell images for machine learning model...
Catherine Sedrani,Paul Wilmes
Catherine Sedrani
The human gut microbiome is crucial for human health and disease but exhibits extensive individual-level strain variation. Distinct strains encode and express different functions. The resulting emergent properties therefore differentially a...
Autologous humanized mouse models of iPSC-derived tumors enable characterization and modulation of cancer-immune cell interactions [0.03%]
自体人类化小鼠模型使来源于人多能干细胞的肿瘤免疫细胞相互作用的表征和调节成为可能
Gaël Moquin-Beaudry,Basma Benabdallah,Damien Maggiorani et al.
Gaël Moquin-Beaudry et al.
Modeling the tumor-immune cell interactions in humanized mice is complex and limits drug development. Here, we generated easily accessible tumor models by transforming either primary skin fibroblasts or induced pluripotent stem cell-derived...
Motif-centric phosphoproteomics to target kinase-mediated signaling pathways [0.03%]
基于基序的磷酸化蛋白质组学用于靶向激酶介导的信号通路
Chia-Feng Tsai,Kosuke Ogata,Naoyuki Sugiyama et al.
Chia-Feng Tsai et al.
Identifying cellular phosphorylation pathways based on kinase-substrate relationships is a critical step to understanding the regulation of physiological functions in cells. Mass spectrometry-based phosphoproteomics workflows have made it p...
Vincent C T Hanlon,Daniel D Chan,Zeid Hamadeh et al.
Vincent C T Hanlon et al.
Single-cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes, and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one-pot (OP) Strand-seq library preparatio...
A Bayesian factorization method to recover single-cell RNA sequencing data [0.03%]
一种用于恢复单细胞RNA测序数据的贝叶斯因子分解方法
Zi-Hang Wen,Jeremy L Langsam,Lu Zhang et al.
Zi-Hang Wen et al.
Single-cell RNA sequencing (scRNA-seq) offers opportunities to study gene expression of tens of thousands of single cells simultaneously, to investigate cell-to-cell variation, and to reconstruct cell-type-specific gene regulatory networks....
Discovery and validation of human genomic safe harbor sites for gene and cell therapies [0.03%]
人类基因组安全港位点的鉴定与验证用于基因及细胞疗法
Erik Aznauryan,Alexander Yermanos,Elvira Kinzina et al.
Erik Aznauryan et al.
Existing approaches to therapeutic gene transfer are marred by the transient nature of gene expression following non-integrative gene delivery and by safety concerns due to the random mechanism of viral-mediated genomic insertions. The disa...