Improved integration of single-cell transcriptome and surface protein expression by LinQ-View [0.03%]
通过LinQ-View改进单细胞转录组和表面蛋白表达的整合
Lei Li,Haley L Dugan,Christopher T Stamper et al.
Lei Li et al.
Multimodal advances in single-cell sequencing have enabled the simultaneous quantification of cell surface protein expression alongside unbiased transcriptional profiling. Here, we present LinQ-View, a toolkit designed for multimodal single...
Taking the time for our bodies: How wearables can be used to assess circadian physiology [0.03%]
花时间了解我们的身体：可穿戴设备在昼夜节律生理评估中的应用
Md Mobashir Hasan Shandhi,Will Ke Wang,Jessilyn Dunn
Md Mobashir Hasan Shandhi
Consumer wearables, such as smart watches, are a promising tool for monitoring circadian health in "real world" settings. Bowman et al. demonstrate that circadian signals can be accurately captured through heart rate data obtained from wear...
Erratum: An optimized genome-wide, virus-free CRISPR screen for mammalian cells [0.03%]
更正：用于哺乳动物细胞的优化型无病毒全基因组CRISPR筛选
Kai Xiong,Karen Julie la Cour Karottki,Hooman Hefzi et al.
Kai Xiong et al.
[This corrects the article DOI: 10.1016/j.crmeth.2021.100062.]. © 2021 The Authors.
Published Erratum
Cell reports methods. 2021 Nov 22;1(7):100115. DOI:10.1016/j.crmeth.2021.100115 2021
Advancing NGS quality control to enable measurement of actionable mutations in circulating tumor DNA [0.03%]
推进NGS质量控制以实现对循环肿瘤DNA中可操作突变的测定
James C Willey,Tom B Morrison,Bradley Austermiller et al.
James C Willey et al.
The primary objective of the FDA-led Sequencing and Quality Control Phase 2 (SEQC2) project is to develop standard analysis protocols and quality control metrics for use in DNA testing to enhance scientific research and precision medicine. ...
Ramsey Bekdash,Jose R Quejada,Shunnosuke Ueno et al.
Ramsey Bekdash et al.
Lactate metabolism has been shown to have increasingly important implications in cellular functions as well as in the development and pathophysiology of disease. The various roles as a signaling molecule and metabolite have led to interest ...
Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry [0.03%]
ChipCytometry技术实现石蜡包埋组织的多重成像及信号定量分析
Sebastian Jarosch,Jan Köhlen,Rim S J Sarker et al.
Sebastian Jarosch et al.
Deciphering the spatial composition of cells in tissues is essential for detailed understanding of biological processes in health and disease. Recent technological advances enabled the assessment of the enormous complexity of tissue-derived...
A deep-learning toolkit for visualization and interpretation of segmented medical images [0.03%]
一种用于可视化和解释分割医学图像的深度学习工具包
Sambuddha Ghosal,Pratik Shah
Sambuddha Ghosal
Generalizability of deep-learning (DL) model performance is not well understood and uses anecdotal assumptions for increasing training data to improve segmentation of medical images. We report statistical methods for visual interpretation o...
Biosensor for deconvolution of individual cell fate in response to ion beam irradiation [0.03%]
用于解析离子束辐照下单细胞命运的生物传感器
Martin Niklas,Julian Schlegel,Hans Liew et al.
Martin Niklas et al.
Clonogenic survival assay constitutes the gold standard method for quantifying radiobiological effects. However, it neglects cellular radiation response variability and heterogeneous energy deposition by ion beams on the microscopic scale. ...
Extracting functional insights from loss-of-function screens using deep link prediction [0.03%]
利用深度链接预测从功能丧失筛选中获取功能见解
Pieter-Paul Strybol,Maarten Larmuseau,Louise de Schaetzen van Brienen et al.
Pieter-Paul Strybol et al.
We present deep link prediction (DLP), a method for the interpretation of loss-of-function screens. Our approach uses representation-based link prediction to reprioritize phenotypic readouts by integrating screening experiments with gene-ge...
Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane [0.03%]
具有工程化标签的四色单分子成像技术揭示了质膜信号复合物的分子结构
Junel Sotolongo Bellón,Oliver Birkholz,Christian P Richter et al.
Junel Sotolongo Bellón et al.
Localization and tracking of individual receptors by single-molecule imaging opens unique possibilities to unravel the assembly and dynamics of signaling complexes in the plasma membrane. We present a comprehensive workflow for imaging and ...