Retrospective cell lineage reconstruction in humans by using short tandem repeats [0.03%]
通过短串联重复序列在人类中进行回顾性细胞系谱重建
Liming Tao,Ofir Raz,Zipora Marx et al.
Liming Tao et al.
Cell lineage analysis aims to uncover the developmental history of an organism back to its cell of origin. Recently, novel in vivo methods utilizing genome editing enabled important insights into the cell lineages of animals. In contrast, h...
A high-content platform for physiological profiling and unbiased classification of individual neurons [0.03%]
一种用于生理特征表征和单个神经元无偏差分类的高内涵平台
Daniel M DuBreuil,Brenda M Chiang,Kevin Zhu et al.
Daniel M DuBreuil et al.
High-throughput physiological assays lose single-cell resolution, precluding subtype-specific analyses of activation mechanism and drug effects. We demonstrate APPOINT (automated physiological phenotyping of individual neuronal types), a ph...
KiRNet: Kinase-centered network propagation of pharmacological screen results [0.03%]
基于激酶的药物筛选结果传播网络模型
Thomas Bello,Marina Chan,Martin Golkowski et al.
Thomas Bello et al.
The ever-increasing size and scale of biological information have popularized network-based approaches as a means to interpret these data. We develop a network propagation method that integrates kinase-inhibitor-focused functional screens w...
Double-jeopardy: scRNA-seq doublet/multiplet detection using multi-omic profiling [0.03%]
双倍危险：利用多组学分析进行scRNAseq双峰/多重检测
Bo Sun,Emmanuel Bugarin-Estrada,Lauren Elizabeth Overend et al.
Bo Sun et al.
The computational detection and exclusion of cellular doublets and/or multiplets is a cornerstone for the identification the true biological signals from single-cell RNA sequencing (scRNA-seq) data. Current methods do not sensitively identi...
Rapid and accurate agglutination-based testing for SARS-CoV-2 antibodies [0.03%]
一种针对SARS-CoV-2抗体的快速且精确测定法
Sally Esmail,Michael J Knauer,Husam Abdoh et al.
Sally Esmail et al.
We have developed a rapid, accurate, and cost-effective serologic test for SARS-CoV-2 virus, which caused the COVID-19 pandemic, on the basis of antibody-dependent agglutination of antigen-coated latex particles. When validated using plasma...
Amplicon residues in research laboratories masquerade as COVID-19 in surveillance tests [0.03%]
研究实验室中的扩增子污染可能在监测测试中被误认为是COVID-19
Dan Davidi,Susan Fitzgerald,Hannah L Glaspell et al.
Dan Davidi et al.
Asymptomatic surveillance testing together with COVID-19-related research can lead to positive SARS-CoV-2 tests resulting not from true infections, but non-infectious, non-hazardous by-products of research (amplicons). Amplicons can be wide...
Profiling human iPSC-derived sensory neurons for analgesic drug screening using a multi-electrode array [0.03%]
人多感觉神经元诱导多能干细胞系的特征分析及药物筛选方法的发展
Chronic pain is a global health issue, yet effective treatments remain limited due to poor preclinical-to-human translation. To address this, we developed a high-content screening (HCS) platform using hiPSC-derived nociceptors to identify a...