EyaHOST, a modular genetic system for investigation of intercellular and tumor-host interactions in Drosophila melanogaster [0.03%]
EyaHOST：一种模块化遗传系统用于研究果蝇（Drosophila melanogaster）的细胞间和肿瘤-宿主相互作用
José Teles-Reis,Ashish Jain,Dan Liu et al.
José Teles-Reis et al.
Studying intercellular and interorgan interactions in animal models is key to understanding development, physiology, and disease. We introduce EyaHOST, a system for clonal combinatorial loss- and gain-of-function genetics in fluorescently l...
Combinatorial responsiveness of chemosensory neurons in mouse explants revealed by DynamicNeuroTracker [0.03%]
小鼠嗅球神经元动态化学感觉联合反应的定量分析
Jungsik Noh,Wen Mai Wong,Bo-Jui Chang et al.
Jungsik Noh et al.
Calcium fluorescence imaging enables us to investigate how individual neurons of live animals encode sensory input or drive specific behaviors. Extracting and interpreting large-scale neuronal activity from imaging data are crucial steps in...
DiffHiChIP: Identifying differential chromatin contacts from HiChIP data [0.03%]
DiffHiChIP：从HiChIP数据中识别差异染色质相互作用
Sourya Bhattacharyya,Daniela Salgado Figueroa,Katia Georgopoulos et al.
Sourya Bhattacharyya et al.
Chromosome conformation capture (3C) assays such as HiChIP are widely used to study interactions between cis-regulatory and structural elements. However, robust methods for detecting condition-specific loops remain limited. We introduce Dif...
Imaging the time course of DNA damage response at a nonrepetitive endogenous locus [0.03%]
在非重复的内源性位点成像DNA损伤反应的时间过程
Adam T Rybczynski,W Taylor Cottle,Po-Ta Chen et al.
Adam T Rybczynski et al.
DNA double-strand breaks (DSBs) are among the most genotoxic lesions. Investigating the cellular dynamics of repair factors during DSB repair requires methodologies that preserve both spatial and temporal information. Here, we describe a me...
A tabletop blast device for the study of the long-term consequences of traumatic brain injury on brain organoids [0.03%]
一种台式爆炸装置 用于研究创伤性脑损伤对类器官长期影响
Riccardo Sirtori,Akash Pandey,Arun Shukla et al.
Riccardo Sirtori et al.
Traumatic brain injury (TBI) is the leading environmental risk factor for neurodegenerative diseases, yet its molecular link to chronic neurodegeneration is unclear. While animal models of TBI are commonly used, emerging research suggests t...
Targeted long-read methylation analysis using hybridization capture suitable for clinical specimens [0.03%]
适用于临床样本的基于杂交捕获的靶向长读段甲基化分析方法
Keisuke Kunigo,Satoi Nagasawa,Keiko Kajiya et al.
Keisuke Kunigo et al.
To detect precise DNA methylation patterns in long-read DNA sequencing analysis, an efficient target enrichment method is needed. In this study, we established t-nanoEM, a practical method that integrates a hybridization-based capture step ...
MetaLigand provides a prior-knowledge-guided framework for predicting non-peptide ligand mediated cell-cell communication [0.03%]
MetaLigand：一种基于先验知识的非肽类配体介导细胞间通讯预测框架
Ying Xin,Yang Jin,Cheng Qian et al.
Ying Xin et al.
Non-peptide ligands (NPLs), including lipids, amino acids, carbohydrates, and non-peptide neurotransmitters and hormones, play a critical role in ligand-receptor-mediated cell-cell communication, driving diverse physiological and pathologic...
SICyLIA-cTMT dissects redox proteome dynamics with high accuracy and depth at microgram scale [0.03%]
基于SIC的Cyanobacteria cTMT氧化蛋白质组技术及应用研究
Sergio Lilla,Samuel Atkinson,Sonja Radau et al.
Sergio Lilla et al.
Cysteine oxidative modifications are critical signaling events regulating cellular functions, but their low abundance and dynamic nature pose technical challenges. We developed the SICyLIA-TMT workflow, which sequentially labels reduced and...
A transmission electron microscopy platform for assessing mitochondrial and nuclear architecture in cardiomyocytes [0.03%]
心肌细胞线粒体和核结构评估的透射电子显微镜平台
Mara Kiessling,Juergen Gindlhuber,Amalia Sintou et al.
Mara Kiessling et al.
Mitochondria are central to cardiomyocyte function, and their spatial organization regulates nuclear signaling and gene transcription, holding potential for novel cardioprotective interventions. We developed a transmission electron microsco...
Single-cell multiomics data integration and generation with scPairing [0.03%]
使用scPairing进行单细胞多组学数据整合和生成
Jeffrey Niu,Carlos Vasquez-Rios,Jiarui Ding
Jeffrey Niu
Single-cell multiomics technologies generate paired measurements of different cellular modalities, such as gene expression and chromatin accessibility. However, multiomics technologies are more expensive than their unimodal counterparts, re...