Resilience of recombinant antibiotic resistance gene-containing plasmids against common cell culture disposal methods [0.03%]
耐药基因重组质粒在细胞培养废弃处理过程中的稳定性研究
Austin Gluth,Christian Zmasek,Stephen Chiu et al.
Austin Gluth et al.
Antibiotics have saved an untold number of people and animals since penicillin's miraculous discovery in 1928. In the following half-century, progressive discoveries involving antibiotic resistance genes (ARGs), the microorganisms responsib...
High-precision stress prediction using deep energy network enhanced by stress equilibrium with Delaunay integration on refined grid [0.03%]
基于精细网格上Delaunay积分的应力平衡增强型深度能量网络高精度应力预测方法
Yongqiang Ye,Dongming An,Hui Li et al.
Yongqiang Ye et al.
We present the deep energy method enhanced by stress equilibrium (DEM-SE), a physics-informed neural network (PINN) architecture for high-precision prediction of complex stress fields in elastic plates. The method calculates total potential...
DRIFT-EM enables direct wafer retrieval of ultrathin serial sections for large-volume electron microscopy [0.03%]
采用大视场电子显微镜直接回收超薄连续切片的漂移方法
Nelson Medina,Joseph V Gogola,Kevin Boergens et al.
Nelson Medina et al.
Large-volume serial electron microscopy (vEM) has transformed neuroscience by enabling reconstructions of neural circuits at synaptic resolution. Approaches to collecting libraries of ultrathin sections required for producing vEM datasets h...
Noninvasive focal gene transfer of chemogenetic proteins in the primate brain [0.03%]
灵长类动物大脑中化学遗传蛋白聚焦非病毒基因转移
Michael R Corigliano,T Vincenza Parks,Simeon S Guretse et al.
Michael R Corigliano et al.
We leverage our recent development of transcranial focused ultrasound (tFUS) for noninvasive, focal delivery of adeno-associated viruses (AAVs)-encoding excitatory designer receptors exclusively activated by designer drugs (DREADDs)-to marm...
Inferring cell-specific gene regulatory networks based on causal graph embedding [0.03%]
基于因果图嵌入的细胞特异性基因调控网络推理方法研究
Guojie Li,Rui Qiao,Yunnuo Xu et al.
Guojie Li et al.
Gene regulatory networks (GRNs) at single-cell resolution provide a fundamental framework for understanding cellular functions and regulatory mechanisms. However, existing methods often focus on regulatory relationships among genes while ov...
Integrated multi-dimensional modeling of non-model bacteria identifies engineering targets for acarbose biosynthesis optimization [0.03%]
整合多维度建模非模式细菌发现 engineered 靶点以优化壳聚糖生物合成
Feifei Cai,Shijie Zhang,Yang Dai et al.
Feifei Cai et al.
Metabolic engineering for high-value compounds such as acarbose, a diabetes drug, requires systematic understanding of metabolic regulation. Here, we applied a multi-dimensional systems biology approach in Actinoplanes sp. SE50/110, a non-m...
Dissecting serum polyclonal antibody escape to SARS-CoV-2 variants by deep mutational learning [0.03%]
通过深度突变分析研究血清多克隆抗体对新冠病毒变异株的逃逸现象
Danielle Shlesinger,Viktor Sadilek,Mason Minot et al.
Danielle Shlesinger et al.
Deep mutational scanning (DMS) has been extensively used to investigate how single-position mutations in the receptor-binding domain (RBD) affect binding of ACE2 and neutralizing antibodies, thus revealing mutations that drive immune escape...
EthoPy provides an accessible platform for reproducible behavioral neuroscience [0.03%]
EthoPy 为可重复的行为神经科学提供了一个便捷的平台
Alexandros Evangelou,Maria Diamantaki,Konstantina Georgelou et al.
Alexandros Evangelou et al.
As brain activity is tightly coupled to behavior, an accurate understanding of neural functions necessitates consideration of behavioral tasks that capture the complexity and variety that animals encounter. Nevertheless, animal training for...
Single-molecule detection methods to study alpha-synuclein aggregation in postmortem Parkinson's disease brains [0.03%]
单分子检测方法研究帕金森病脑死后α-突触核蛋白聚集
Emre Fertan,John S H Danial,Stephen Neame et al.
Emre Fertan et al.
Nanoscopic aggregates of alpha-synuclein (ɑSyn) have been observed in Parkinson's disease (PD). However, the processes that occur in vivo leading to the formation of these small aggregates are not well understood. We used ultra-sensitive s...
Fast and efficient Borrelia chromosome recovery from tick samples using whole-genome amplification [0.03%]
使用全基因组扩增法从蜱样本中快速高效地获取伯氏疏螺旋体染色体
Sophie Melis,Cristina Cezara Bunduc,Laura Mottarlini et al.
Sophie Melis et al.
Genomic studies of Borrelia are essential for understanding Lyme borreliosis epidemiology and biology but are limited by slow, labor-intensive culture methods. Here, we introduce an approach that overcomes the need for extended culture by c...