Improved expansion of peripheral blood and iPSC-derived natural killer cells for clinical applications [0.03%]
用于临床应用的外周血和iPSC来源的自然杀伤细胞的扩增改进
Jaya Lakshmi Thangaraj,Edith Lopez,Dan S Kaufman
Jaya Lakshmi Thangaraj
The large-scale expansion of natural killer (NK) cells commonly relies on artificial antigen-presenting cells (aAPCs). Currently, K562 cells expressing 41BBL and membrane-bound IL-21 (mbIL-21) are widely used to expand NK cells for clinical...
Data modeling the interplay between single-cell shape, single-cell protein expression, and tissue state [0.03%]
单细胞形态、单细胞蛋白表达和组织状态之间的相互作用的数据建模
Yuval Tamir,Yuval Bussi,Claudia Owczarek et al.
Yuval Tamir et al.
While cell shape fundamentally governs tissue function, the underlying links between single-cell shape and protein expression have been difficult to resolve due to limitations in imaging multiplexing and population averaging. Here, we use m...
Wei Liu,Dewei Xiang,Xiaolu Jiang et al.
Wei Liu et al.
SpaMIE is a deep graph neural network framework designed to tackle the challenge of multi-section integration in spatial multi-omics (SMO) datasets with systematic missing modalities. Current SMO platforms face limitations such as high cost...
Nina Tekkey,John E Garza,Wenjin Zhang et al.
Nina Tekkey et al.
When aligning next-generation sequencing (NGS) reads to a reference genome, differences between the true genome of the individual under study and the reference result in a biased interpretation of aligned data through systematic errors know...
Alicia Rey,Francesco Petrelli,Diana Panfilova et al.
Alicia Rey et al.
Lipid droplets (LDs) are lipid-storage organelles that have gained interest in neurodegenerative diseases, yet their physiological role in the brain is not fully understood. Classical LD detection using lipophilic dyes like BODIPY 493/503 (...
Tumor immune microenvironment reconstitution in patient-derived organoids enables therapy modeling for NSCLC [0.03%]
基于患者的类器官肿瘤免疫微环境的重建可实现对非小细胞肺癌的治疗建模
Enrique Podaza,Jared Capuano,Hui-Hsuan Kuo et al.
Enrique Podaza et al.
Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality. Despite various therapeutic options, treatment resistance is common, underscoring the need for effective combination therapies and reliable pre-clinical...
HyPIC-3D enables characterization of migratory cancer cell subpopulations in 3D hypoxic microenvironments [0.03%]
HyPIC-3D可在三维缺氧微环境中表征迁移性癌细胞亚群
Luana Schito,Sergio Rey-Keim
Luana Schito
Malignant tumors harbor cancer cell subpopulations with heterogeneous migratory behaviors. Identifying the molecular programs specifying whether these subpopulations migrate or remain stationary is essential for preventing metastasis and re...
In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier [0.03%]
用于活体可视化生物活性铁和氧的LiON（游离铁和氧示踪剂）标记体系
Ayato Maeda,Akihiro Nita,Shoko Sashiyama et al.
Ayato Maeda et al.
Iron and oxygen are essential for numerous biological processes, yet monitoring their bioactive intracellular dynamics in vivo remains challenging due to the limitations of current detection methods. Here, we introduce the labile iron and o...
A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study [0.03%]
基于多重PCR的测序方法用于血浆样本诊断和区分泡型和囊型包虫病的概念验证研究
Yanping Zhao,Yeqin Wang,Ming Zhi et al.
Yanping Zhao et al.
Echinococcosis poses a significant public health threat, and its diagnosis relies on imaging. Non-invasive plasma detection of Echinococcus cell-free DNA (cfDNA) offers a promising diagnostic approach. We developed a low-cost multiplex PCR ...
Human cerebral organoids with microglia and vasculature model glioma stem cell interactions and radiotherapy response [0.03%]
含小胶质细胞和血管的脑类器官模拟胶质瘤干细胞相互作用及放射治疗反应
Jérémy Raguin,Noa Legrand,Thierry Kortulewski et al.
Jérémy Raguin et al.
Most human brain organoid models derived from induced pluripotent stem cells (iPSCs) lack vascular and/or immune components, despite their critical roles in maintaining brain homeostasis and contributing to pathophysiological processes. We ...