Visual detection of seizures in mice using supervised machine learning [0.03%]
基于监督机器学习的癫痫发作视觉检测方法研究——以小鼠模型为例
Gautam S Sabnis,Leinani Hession,J Matthew Mahoney et al.
Gautam S Sabnis et al.
Seizures are caused by abnormal synchronous brain activity. The resulting changes in muscle tone, such as twitching, stiffness, or jerking, are used in visual scoring systems such as the Racine scale to quantify seizure intensity. However, ...
Real-time 4D MRI reconstruction using DVR-NeMF, a framework for dynamic volumetric reconstruction [0.03%]
用于动态体积重建的实时4D磁共振成像重构框架DVR-NeMF
Ruoxi Wang,Sijie Zhong,Jincheng Li et al.
Ruoxi Wang et al.
In this study, four dynamic magnetic resonance imaging (MRI) sequences were first developed to collect data. On this basis, we presented DVR-NeMF, a neural magnetic field framework that enables real-time, high-dimensional (4D) MRI reconstru...
Vector-guided graph learning for spatial multi-slice multi-omics alignment [0.03%]
向量引导的图学习在空间多层多组学对齐中的应用
Yikai Lou,Xuan Li,Qixing Yang et al.
Yikai Lou et al.
Spatial mapping of multi-slice multi-omics data enables the identification of shared and slice-specific cellular components across spatiotemporal axes. However, conventional graph neural networks assume uniform contributions from neighborin...
Temporal reassignment and correspondence evaluation with quality control for time-course imaging of 3D cell culture [0.03%]
具有质量控制的时间进程成像的时空重新分配和对应评估（用于3D细胞培养）
Eric M Cramer,Tamara Lopez-Vidal,Jeanette Johnson et al.
Eric M Cramer et al.
Longitudinal imaging of 3D cell cultures like tumor organoids and spheroids offers crucial insights into cancer progression and treatment. However, spatial displacement during time-course imaging, caused by matrix detachment or experimental...
Fast and sensitive detection of targeted gene fusions using frequency minimizers and fuzzy pattern matching with Fuzzion2 [0.03%]
利用Fuzzion2频率最小化和模糊模式匹配技术快速灵敏地检测特定基因融合现象
Stephen V Rice,Michael N Edmonson,Xiaolong Chen et al.
Stephen V Rice et al.
To enable fast and sensitive fusion detection critical for clinical oncology testing, we developed Fuzzion2, a pattern-matching program for detecting targeted gene fusions that employs an index of frequency minimizers and fuzzy matching to ...
Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs [0.03%]
皮质类器官衍生的黑色素瘤脑转移生态位模型可优先选择抗癌药物
Kim Krieg,Silvia Materna-Reichelt,Tobias Naber et al.
Kim Krieg et al.
Effective systemic therapies against brain metastases are severely limited. To understand and target vulnerabilities of human metastases in a brain niche context, we developed reproducible melanoma brain metastasis (MBM) models for metastas...
Non-invasive measurement of biomolecular condensate interfacial tension and bending rigidity [0.03%]
生物分子凝聚体界面张力和弯曲刚度的无创测量
Thomas A Williamson,Jack O Law,Thomas Stevenson et al.
Thomas A Williamson et al.
Accurate measurement of biomolecular condensates' mechanical properties is essential to understand their behavior within cells. We present FlickerPrint, an open-source Python package to determine the interfacial tension and bending rigidity...
Simultaneous capture of single cell RNA-seq, ATAC-seq, and CRISPR perturbation enables multiomic screens to identify gene regulatory relationships [0.03%]
同时捕获单细胞RNA测序、ATAC测序和CRISPR扰动可进行多组学筛选以识别基因调控关系
Kaivalya Shevade,Yeqing Angela Yang,Kevin Feng et al.
Kaivalya Shevade et al.
Here, we introduce CRISPR and transcriptomics-assay for transposase-accessible chromatin (CAT-ATAC), a technique that adds CRISPR guide RNA (gRNA) capture to the existing 10× Genomics Multiome assay, generating linked transcriptome, chroma...
High-speed neural imaging with multiplexed miniaturized two-photon microscopy [0.03%]
采用多路复用微型化双光子显微镜进行高速神经成像
Zixiao Zhang,Shing-Jiuan Liu,Ben Mattison et al.
Zixiao Zhang et al.
Head-mounted miniaturized two-photon microscopes enable cellular-resolution recording of neural activity deep in the mouse brain during unrestrained behavior. Two-photon microscopy, however, is traditionally limited in frame rate by the nec...
Benchmarking of human read removal strategies for viral and microbial metagenomics [0.03%]
评估人类宿主序列去除策略在病毒和微生物元基因组分析中的性能基准测试
Matthew Forbes,Duncan Y K Ng,Róisín M Boggan et al.
Matthew Forbes et al.
Human reads are a key contaminant in microbial metagenomics and enrichment-based studies, requiring removal for computational efficiency, biological analysis, and privacy protection. Various in silico methods exist, but their effectiveness ...