Development of far-red fluorescent proteins for temporal domain multiplexing and super-resolution imaging [0.03%]
远红荧光蛋白的发展应用于时间领域多路复用和超分辨率成像
Olumayowa Fakorede,Zhien Rong,Ruizhao Wang et al.
Olumayowa Fakorede et al.
The diverse spectral and photochemical properties of fluorescent proteins enable imaging applications ranging from organelle labeling to super-resolution and multiplexed live-cell microscopy. Here, we report three far-red fluorescent protei...
Detecting protein higher-order structural changes using kinase as a phospho-labeler [0.03%]
基于蛋白激酶的磷酸化标记检测蛋白质高阶结构变化的新方法
Asato Maeda,Kosuke Ogata,Yasushi Ishihama
Asato Maeda
We developed an approach to detect protein structural changes on a proteome-wide scale by phosphate labeling through in vitro kinase reactions. This structural proteomics approach combines residue-specific labeling based on kinase substrate...
Real-time cell viability monitoring for high-throughput drug screening using tumor xenograft-derived cells [0.03%]
基于移植瘤细胞的高通量药物筛选实时细胞存活监测技术研究
Elham Esmaeilishirazifard,Daniel Guerrero-Romero,Allan J W Lui et al.
Elham Esmaeilishirazifard et al.
Patient-derived tumor xenografts (PDTXs) recapitulate the molecular and phenotypic heterogeneity of human cancers, making them valuable pre-clinical models for cancer drug development. However, high-throughput drug screening (HTDS) using ex...
Toward simultaneous pseudo-space reconstruction and cell-type deconvolution of single-cell spatial transcriptome using SpaDicer [0.03%]
基于SpaDicer的单细胞空间转录组伪bulk重建与细胞类型去卷积
Junming Zhang,Shi Yin,Lingxi Xu et al.
Junming Zhang et al.
Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) offer complementary insights into tissue heterogeneity. Each modality is characterized by inherent trade-offs: scRNA-seq offers high resolution but lacks spatial contex...
Parallel gene amplification by Cas9 nickase for generating functionally heterogeneous cell populations [0.03%]
Cas9切刻酶的平行基因扩增以生成功能异质性细胞群
Hiroaki Takesue,Satoshi Okada,Takashi Ito
Hiroaki Takesue
Genetic diversity underlies adaptive evolution. Because genes often act in concert to execute biological processes, the dosage stoichiometry among cooperating genes represents an additional layer of diversity beyond sequence variation. We t...
Atomic elementary flux modes explain the steady state flow of metabolites in large-scale flux networks [0.03%]
原子基本通量模式解释了大型通量网络中代谢物稳态流动的现象
Justin G Chitpin,Theodore J Perkins
Justin G Chitpin
Steady state fluxes are a measure of cellular activity under homeostatic conditions, but understanding how individual substrates are metabolized remains a challenge in large-scale networks. Pathway-based approaches such as elementary flux m...
ATACompass enables cross-species cell identity mapping for scATAC-seq without gene annotations [0.03%]
基于scATAC-seq的跨物种细胞身份映射工具ATACompass无需基因注释
Jie Jiang,Yali Ding,Bu Jin et al.
Jie Jiang et al.
Single-cell assay for transposase-accessible chromatin with sequencing (scATAC-seq) provides insights into transcriptional regulation, but there remain challenges in cell identity annotation due to data sparsity and limited gene regulatory ...
A generative framework for predicting cellular morphological and transcriptomic perturbation responses [0.03%]
一种预测细胞形态和转录组干扰反应的生成框架
Rui Peng,Ziru Liu,Yuanxu Gao et al.
Rui Peng et al.
Predicting coordinated cellular responses to perturbations is crucial for phenotypic drug discovery and building virtual cell models. Current methods typically predict isolated modalities or require empirical post-perturbation data, preclud...
Andriana Charalampopoulou,Arens Taga,Khalil Rust et al.
Andriana Charalampopoulou et al.
Degeneration of the corticospinal tract is a feature in several neurodegenerative disorders and leads to disability. However, modeling corticospinal neuron (CSN) pathology and corticospinal connectivity is challenging, as there are interspe...
Reproducible detection of antigen-specific T cells and Tregs via standardized and automated activation-induced marker assay workflows [0.03%]
通过标准化和自动化活化诱导标志物测定工作流程可重复鉴定抗原特异性T细胞和调节性T细胞
Torin Halvorson,Gabrielle Boucher,Daniel Yokosawa et al.
Torin Halvorson et al.
Activation-induced marker (AIM) assays are a promising tool to track antigen-specific T cells, but methodological heterogeneity between research groups hinders their clinical utility. To evaluate AIM assay reproducibility, we conducted a mu...