Dissecting novel object exploration in a fully automated homecage-based novel object recognition test [0.03%]
基于全自动化笼养制的新物体识别测试中的新物体探索行为分析
Hinze Ho,Nejc Kejzar,Stephen Burton et al.
Hinze Ho et al.
The novel object recognition (NOR) test is widely used to assess memory in rodents, offering strong ethological validity, cross-species relevance, and specificity for hippocampal-parahippocampal function. However, standard implementations a...
Epigenomic, transcriptomic, and proteomic characterization of breast cancer cell line reference samples [0.03%]
乳腺癌细胞系参考样品的表观基因组、转录组和蛋白质组特征刻画
Chirag Nepal,Wanqiu Chen,Zhong Chen et al.
Chirag Nepal et al.
Next-generation sequencing requires accuracy, reproducibility, and standardized reference materials. The Sequencing Quality Control (SEQC-2) multicenter studies on paired breast cancer and B cell lines generated extensive genomic datasets, ...
Improved flux profiling in genome-scale modeling of human cell metabolism [0.03%]
改进人类细胞代谢基因组规模模型中的通量分析
Cyriel A M Huijer,Xiang Jiao,Yun Chen et al.
Cyriel A M Huijer et al.
Understanding human cell metabolism through genome-scale flux profiling is of interest to diverse research areas of human health and disease. Metabolic modeling using genome-scale metabolic models (GEMs) has the potential to achieve this, b...
Evaluation of the effects of a new standard equation for doubly labeled water studies [0.03%]
评估新型双重标记水研究标准方程的效果
Kaja Falkenhain,Jennifer C Rood,Corby K Martin et al.
Kaja Falkenhain et al.
Accurate measurement of energy expenditure is critical for metabolic research and public health. Doubly labeled water (DLW) is the gold standard for assessing free-living energy expenditure, yet inconsistencies in equations impede comparabi...
Homomorphic encryption enables privacy preserving polygenic risk scores [0.03%]
同态加密技术使隐私保护型多基因风险评分的计算成为可能
Elizabeth Knight,Jiaqi Li,Matthew Jensen et al.
Elizabeth Knight et al.
Polygenic risk score models (PRSs) are important tools in precision medicine, enabling personalized risk prediction; however, they raise privacy concerns. Fully homomorphic encryption (FHE) provides a potential solution, allowing computatio...
Generation of thymus-reconstituting T cell progenitors from human pluripotent stem cells [0.03%]
人多能干细胞向胸腺定植T细胞前体的诱导生成
Elena S Philonenko,Baoyun Zhang,Eugene Albert et al.
Elena S Philonenko et al.
Generating a large number of progenitors that can repopulate the immune system of a recipient is one of the key steps toward efficient cancer immunotherapy. Here, we describe the engineering of T cell progenitors capable of direct and long-...
Assessing PARP trapping dynamics in ovarian cancer using a CRISPR-engineered FRET biosensor [0.03%]
利用CRISPR工程FRET生物传感器评估卵巢癌中PARP捕获动力学
Daniel Marks,Edwin Garcia,Sunil Kumar et al.
Daniel Marks et al.
Poly(ADP-ribose) polymerase inhibitors (PARPi) have revolutionized the treatment of ovarian high-grade serous carcinoma (HGSC), particularly in homologous recombination-deficient tumors. However, the emergence of resistance poses a critical...
Cláudia Martinho,Masakazu Hoshino,Morgane Raphalen et al.
Cláudia Martinho et al.
Brown algae represent the third most complex lineage to have independently evolved multicellularity, distinct from plants and animals. Yet, functional studies of their development and evolution have been limited by the absence of efficient ...
In vivo AGO-APP for cell-type- and compartment-specific miRNA profiling in the mouse brain [0.03%]
体内AGO-APP在小鼠大脑细胞类型和区室特异性miRNA谱分析中的应用
Surbhi Kapoor,Andrea Erni,Francesca Vincenzi et al.
Surbhi Kapoor et al.
AGO-APP through the expression of the T6B peptide permits the isolation of Ago-bound microRNAs (miRNAs). Here, we present the generation and characterization of two transgenic mouse lines that enable AGO-APP to be performed in vivo. First, ...
MMonitor for real-time monitoring of microbial communities using long reads [0.03%]
基于长读段的实时监测微生物种群的方法/MMonitor方法
Timo N Lucas,Ulrike Biehain,Anupam Gautam et al.
Timo N Lucas et al.
Real-time monitoring of microbial communities offers valuable insights into microbial dynamics across diverse environments. However, many existing metagenome analysis tools require advanced computational expertise and are not designed for m...