Neural barcoding representing cortical spatiotemporal dynamics based on continuous-time Markov chains [0.03%]
基于连续时间马尔可夫链的神经条形码表示皮层时空动力学
Jordan M Culp,Donovan M Ashby,Antis G George et al.
Jordan M Culp et al.
Populations of neurons form assemblies at many scales and display recurring spatiotemporal patterns of activity. In the cerebral cortex, these patterns of activity involve coordinated activity spanning large distances and anatomical regions...
Using DIPA-CRISPR for simple and efficient endogenous protein tagging in insects [0.03%]
利用DIPA-CRISPR对昆虫内源蛋白进行简便而高效的标记
Alfonso Ferrández-Roldán,Maria-Dolors Piulachs
Alfonso Ferrández-Roldán
CRISPR-Cas9 is rapidly expanding across diverse organisms. Among these advances, in-frame knockins of reporter genes have become essential for studying gene expression and protein localization. However, in hemimetabolan insects such as the ...
John Hageter,Audrey DelGaudio,Maegan Leathery et al.
John Hageter et al.
Functional imaging using genetically encoded indicators has become a foundational tool for cellular dynamics and communication analysis. However, large or complex experiments pose analytical challenges. Many programs address these challenge...
African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementation [0.03%]
非洲侏鼠诱导多能干细胞作为体外胚胎发生、种间嵌合体和囊胚补偿的模型
Pjeter Gjonlleshaj,Ajda Lenardič,Monika Tarnowska-Sengül et al.
Pjeter Gjonlleshaj et al.
Somatic cell reprogramming into induced pluripotent stem cells (iPSCs) has been achieved in various mammals; however, assessing iPSC contribution to full-term chimeras beyond laboratory rodents remains challenging. Here, we demonstrate indu...
Investigation of synaptic connectivity in functional in vitro neuronal assemblies [0.03%]
用于功能体外神经元组装的突触连接的研究
Clara Zaccaria,Asiye Malkoç,Ilya Auslender et al.
Clara Zaccaria et al.
Studies using genetic tagging and optogenetics demonstrated that reactivation of memory engrams, neuronal ensembles encoding specific learned information, can trigger memory recall and that synaptic potentiation among engram neurons is crit...
Fluorescent indicators for visualizing dynamic contact between cells and between processes originating from a single cell [0.03%]
用于检测细胞间动态接触以及起源于单个细胞的过程之间动态接触的荧光指示剂
Takashi Kanadome,Natsumi Hoshino,Susumu Jitsuki et al.
Takashi Kanadome et al.
Cells continuously communicate through dynamic cell-cell contacts. Tools for visualizing these dynamic interactions in living cells are essential to the study of fundamental biological processes in multicellular organisms. Here, we present ...
Evaluation of statistical differential analysis methods for identification of senescent cells using single-cell transcriptomics [0.03%]
基于单细胞转录组学的衰老细胞鉴定的统计差异分析方法评估
Dongmei Li,Pinxin Liu,Irfan Rahman et al.
Dongmei Li et al.
Differential gene expression (DGE) analysis is a crucial step in identifying senescent cells using single-cell RNA sequencing (scRNA-seq) data. However, few studies have evaluated the performance of DGE methods-particularly those implemente...
High-efficiency transfection of Acanthamoeba castellanii using a cationic polymer [0.03%]
阳离子聚合物高效转染卡氏棘阿米巴细胞
Anaísa B Moreno,Viktor Ek,Jens Eriksson et al.
Anaísa B Moreno et al.
The free-living amoeba Acanthamoeba castellanii is an ecologically, clinically, and evolutionarily important microorganism. A. castellanii amoebae can be directly pathogenic to humans and serve as reservoirs for bacterial pathogens (e.g., L...
Simultaneous determination of free and total metabolite concentrations in proteinaceous specimens by 1D 1H CPMG NMR [0.03%]
基于一维CPMG谱的样品中游离型和结合型代谢物的同时定量检测方法研究
Alexander Reindl,Claudia Samol,Silke Haerteis et al.
Alexander Reindl et al.
Nuclear magnetic resonance (NMR) spectroscopy is often used for the analysis of metabolites in proteinaceous biological specimens. However, the binding of metabolites to proteins impedes accurate quantitation of total metabolite concentrati...
HT SpaceM enables high-throughput mapping of metabolic diversity at the single-cell level [0.03%]
HT SpaceM 实现了单细胞水平代谢多样性的高通量绘图
Rune Daucke,Erwin M Schoof
Rune Daucke
Expanding metabolomic profiling to the single-cell level can reveal metabolic heterogeneity and clinically relevant subpopulations, yet existing methods lack sensitivity and scale. To address this gap, in a recent issue of Cell, Delafiori a...