Framework for evaluating in vitro vasculogenic mimicry using structural and metabolic parameters [0.03%]
基于结构和代谢参数的体外血管生成模拟评价体系的建立框架
Gema Nicolle Santander,Nicole Babbitt,Javiera Veas-Torres et al.
Gema Nicolle Santander et al.
Vasculogenic mimicry (VM) refers to the ability of non-endothelial cells to form fluid-conducting, vessel-like structures independently of endothelial cells. In cancer, VM is associated with tumor aggressiveness and poor prognosis, although...
A standardized and reproducible behavioral framework to elicit multiple aspects of visual spatial attention in mice [0.03%]
一种在小鼠中诱发视觉空间注意力的多个方面的标准化和可重复的行为框架
Kayla Peelman,Nicole Allen,Jamin Ahn et al.
Kayla Peelman et al.
Understanding how neural activity gives rise to cognitive processes such as selective attention is a fundamental goal of neuroscience. An important but often overlooked advance toward this goal requires the development and sharing of rigoro...
TACTIC: A transfer learning framework to predict drug interactions in emerging pathogens [0.03%]
一种预测新发传染病用药相互作用的迁移学习框架 Tactical
Carolina H Chung,David C Chang,Nicole M Rhoads et al.
Carolina H Chung et al.
Machine learning (ML) is necessary to efficiently identify potent drug combinations within a large candidate space to combat drug resistance. However, existing ML approaches cannot be applied to emerging and understudied pathogens with limi...
Large-scale discovery platform enables identification of peptides targeting drug-resistant candidiasis [0.03%]
大规模发现平台能够识别针对耐药性念珠菌病肽类药物
Bahar Behsaz,Abhinav Adduri,Mustafa Guler et al.
Bahar Behsaz et al.
Natural products have an unparalleled track record as sources of clinical drugs. Among them, nonribosomal peptides (NRPs) stand as one of the most therapeutically significant classes, encompassing numerous approved anti-infective and antica...
A computational method to design broad-spectrum T cell-inducing vaccines applied to Betacoronaviruses [0.03%]
一种针对贝塔冠状病毒设计广谱T细胞诱导型疫苗的计算方法
Phil Palmer,Sofiya Fedosyuk,Srivatsan Parthasarathy et al.
Phil Palmer et al.
Antigenically diverse pathogens such as coronaviruses pose substantial global health threats, highlighting the need for broad-spectrum vaccines. Here, we introduce Spectravax, a computational method that designs broad-spectrum vaccines acco...
MalDeepSeq panel: A targeted ultra-deep sequencing approach to trace drug resistance markers in Plasmodium falciparum [0.03%]
针对恶性疟原虫抗药性标记物追踪的靶向超高深度测序方法(MalDeepSeq)面板
Yanka E A R Salazar,Antonio M Rezende,Maria C S B Puça et al.
Yanka E A R Salazar et al.
The emergence of drug-resistant Plasmodium falciparum highlights the need for tools to detect minor parasite subpopulations before resistant lineages expand. We developed and validated a targeted ultra-deep sequencing framework for the full...
Induced pluripotent stem cell-derived macrophages enable broad modeling of human inflammasome signaling [0.03%]
诱导多能干细胞源性巨噬细胞可广泛模拟人类炎性小体信号转导
Chloe M McKee,Melanie Cranston,Emma C McKay et al.
Chloe M McKee et al.
Macrophage models are a mainstay of inflammasome research; however, current in vitro models have significant limitations. Here, we generate induced pluripotent stem cell (iPSC)-derived macrophages (iMacs) to study inflammasome signaling and...
Rapid discovery of cell-surface glycosylation regulators using a lectin-based magnetic CRISPR screen [0.03%]
基于凝集素的磁性CRISPR筛选快速发现细胞表面糖基化调节因子
Jimmy Kim,Halen Kovacs,Simon Wisnovsky
Jimmy Kim
FACS-based CRISPR screening has emerged as a potent tool for dissecting the genetic networks that regulate cell-surface glycosylation. However, existing protocols can be tedious and are not compatible with many cell models. We developed a l...
A real-time FRET ubiquitin transfer assay for quantitative characterization of ternary complexes in targeted protein degradation [0.03%]
用于定量表征靶向蛋白降解三元复合物的实时FRET泛素转移测定法
Corey H Yu,Vi Dougherty,Dongwen Lv et al.
Corey H Yu et al.
Inhibiting or redirecting E3 ubiquitin ligase activity with drug-like small molecules is of growing therapeutic interest, yet broadly applicable and robust assays for quantifying E3-catalyzed ubiquitination remain limited. Here, we present ...
A high-throughput, end-to-end pipeline for extracellular miRNA biomarker discovery from human biofluids [0.03%]
一种高效的体外microRNA生物标志物发现的端到端流程：从人体生物流体分析入手
Abbas Hakim,Jennifer N Chousal,Srimeenakshi Srinivasan et al.
Abbas Hakim et al.
We developed a scalable pipeline for extracellular miRNA (ex-miRNA) profiling that integrates automated exRNA extraction, small RNA sequencing, and bioinformatic analysis including data processing and normalization. Automated extraction pro...