2P-FENDO-II: A fiber bundle microscope for all-optical, large field-of-view brain studies in freely moving mice [0.03%]
2P-FENDO-II：一种全光学、大视野自由活动小鼠脑成像纤维束显微镜
François G C Blot,Dimitri Decombe,Antonio Lorca-Cámara et al.
François G C Blot et al.
All-optical strategies enable identification of functional neuronal ensembles with calcium imaging and replay/alter their spatiotemporal activity with optogenetics to decipher their behavioral implications. We previously developed a fiber-c...
HazardPyMatch: A tool for identifying reproductive and other hazards in scientific laboratories [0.03%]
HazardPyMatch：一种在科学实验室中识别生殖及其他危害的工具
Emily M Parker,Anastasia-Maria Zavitsanou,Clara Liff et al.
Emily M Parker et al.
Understanding and mitigating laboratory hazards is essential for fostering safe and inclusive research environments. However, conducting risk assessments can be challenging and time consuming, especially for scientists who have new or speci...
A unified framework combining linear and 3D molecular features for robust drug-protein interaction prediction [0.03%]
一种结合线性和3D分子特征的用于稳健药物-蛋白质相互作用预测的统一框架
Chang Sun,Zichen Qin,Minglei Li et al.
Chang Sun et al.
We develop PointDPI to predict drug-protein interactions by simultaneously exploiting their linear and 3D structural features. By aligning the features, PointDPI stereoscopically recognizes molecular properties and reduces reliance on 3D st...
An integrative spatial multi-omic workflow for unified analysis of tumor tissue [0.03%]
一种集成的空间多组学工作流程，用于统一分析肿瘤组织
Jurgen Kriel,Joel J D Moffet,Tianyao Lu et al.
Jurgen Kriel et al.
Combining molecular profiling with imaging techniques has advanced the field of spatial biology, offering new insights into complex biological processes. Focusing on diffuse IDH-mutated glioma, this study presents a workflow for spatial mul...
An orthogonal CRISPR/Cpf1 platform for precise spatiotemporal gene regulation and osteoporotic fracture repair [0.03%]
一个正交CRISPR/Cpf1平台用于精准时空基因调控和治疗骨质疏松性骨折
Jie Zhao,Zengliang Wang,Lina Lu et al.
Jie Zhao et al.
CRISPR-Cas systems enable powerful gene editing and regulation, yet single-modality control often fails to achieve orthogonal, spatiotemporally precise regulation of multiple endogenous genes. We engineered OREC, an orthogonal platform inte...
N Suhas Jagannathan,Wei-Xiang Sin,Denise Bei Lin Teo et al.
N Suhas Jagannathan et al.
We present a modeling framework that can perform real-time estimation of per-cell metabolic rates of T cells expanded ex vivo in a reactor. We validate our estimated rates using metabolic assays, show how average rates can be deconvoluted t...
Predictive modeling of molecular activity underlying physical cell-cell interactions [0.03%]
预测分子活性的模型构建及其在物理细胞-细胞相互作用中的应用
Tamjeed Azad,Sarah K Walker,Gabriel D Victora et al.
Tamjeed Azad et al.
Interactions between cells are central to tissue organization and function in health and disease. Labeling immune partnerships by sortagging intercellular contacts (LIPSTIC) quantitatively measures direct physical cell-cell interactions. Co...
AxoMetric platform facilitates rapid and unbiased automated quantification of axon regeneration in tissue sections [0.03%]
AxoMetric平台可实现组织切片中轴突再生的快速而无偏差的自动化量化
Matthew C Finneran,Tara Rahmani,Ismaël Valentin Salioski et al.
Matthew C Finneran et al.
Experimental strategies to stimulate axon regeneration are the core of therapeutic development for nervous system injury and disease. Here, we present AxoMetric, an open-source, user-friendly software for rapid and automated quantification ...
DMC-BrainMap is an open-source, end-to-end tool for multi-feature brain mapping in different species [0.03%]
DMC-BrainMap是一个开源的、端到端的跨物种多模态脑图谱工具
Felix Jung,Xiao Cao,Loran Heymans et al.
Felix Jung et al.
Rigid anatomical mapping is a necessity in current neuroscience research. Here, we introduce DMC-BrainMap, an open-source napari plugin designed as a user-friendly tool for streamlined processing and whole-brain analysis of anatomical data....
Multi-omic analysis of guided and unguided forebrain organoids reveals differences in cellular composition and metabolic profiles [0.03%]
定向和非定向诱导的脑器官的多组学分析揭示了细胞组成和代谢谱型的不同
Marie Sejberg Øhlenschlæger,Pia Jensen,Jesper Foged Havelund et al.
Marie Sejberg Øhlenschlæger et al.
Neural organoids are invaluable model systems for studying neurodevelopment, generated by either guided or unguided approaches. Despite the importance for the field, the resulting differences between these models are unclear. To obtain an u...