Yash Patel,Chenghao Zhu,Takafumi N Yamaguchi et al.
Yash Patel et al.
The price, quality, and throughput of DNA sequencing continue to improve. Algorithmic innovations have allowed inference of a growing range of features from DNA sequencing data, quantifying nuclear, mitochondrial, and evolutionary aspects o...
Chuanbin Su,Han-Seul Ryu,Keerthivasan Raanin Chandradoss et al.
Chuanbin Su et al.
More than 60 human disorders are caused by unstable expansion of short tandem repeat (STR) tracts. These can exhibit cell-type-specific mosaicism in several repeat expansion disorders and remain difficult to characterize due to technical ch...
Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples [0.03%]
Yang Bai,Yuqing Wang,Yicheng Fu et al.
Yang Bai et al.
Single-cell metabolic characteristics are powerful indicators of cellular physiological and pathological states. Flow cytometry enables high-throughput single-cell metabolic profiling and immunophenotyping; however, spectral overlap impedes...
Anamika Yadav,Kyle Alvarez,Akanimoh Adeleye et al.
Anamika Yadav et al.
We present TLPath, a deep learning framework that predicts bulk-tissue telomere length from tissue morphology extracted from routine histopathology images. The central thesis of TLPath is that telomere length can be determined from tissue m...
Lucien Hinderling,Roman Schwob,Guillaume Witz et al.
Lucien Hinderling et al.
We present Convpaint, a universal computational framework for interactive pixel classification. Convpaint uses pretrained convolutional neural networks (CNNs), vision transformers (ViTs), or classical filter banks for feature extraction in ...
Comprehensive cellular analysis with single-nucleus RNA-seq of archived PAXgene whole blood samples [0.03%]
Ojasvi Chaudhary,Mia Steinberg,Grant Duclos et al.
Ojasvi Chaudhary et al.
Single-cell analysis of the large repositories of preserved whole blood samples stored in PAXgene blood RNA tubes has not yet been available. To address this gap, two nuclei-isolation techniques for single-nucleus RNA sequencing were evalua...
MitoTracker transfers from astrocytes to neurons independently of mitochondria [0.03%]
线粒体独立的星形胶质细胞向神经元转移
Katriona L Hole,Rosalind Norkett,Emma Russell et al.
Katriona L Hole et al.
The neuroprotective transfer of mitochondria from astrocytes to neurons has been primarily investigated by labeling astrocytic mitochondria with the dye MitoTracker. Here, we labeled astrocytic mitochondria with both a genetically encoded f...
Practical AI-based cell extraction and spatial statistics for large 3D bone marrow tissue images [0.03%]
基于AI的大规模三维骨髓组织图像细胞提取和空间统计学的实践
George Adams,Floriane S Tissot,Chang Liu et al.
George Adams et al.
Although the molecular regulation of hematopoiesis is well characterized, the spatial organization of hematopoietic cells within bone marrow (BM) remains unclear. Advances in microscopy have produced increasingly detailed images of murine B...
Joana Dopp,Sarah Martens,Frederik Hobin et al.
Joana Dopp et al.
Astrocytes are the brain's major glial population and have been associated with a vast number of functions. To probe this diversity and to reach a similar level of understanding about astrocyte physiology that we have about neurons, we need...
A streamlined, nanopore-compatible 5PSeq protocol for rapid phenotypic antimicrobial sensitivity testing [0.03%]
一种快速的表型抗菌素敏感性检测方法5PSeq的精简版纳米孔兼容协议
Honglian Liu,Susanne Huch,Ryan Hull et al.
Honglian Liu et al.
Antimicrobial resistance (AMR) poses a significant threat to public health. Rapid and accurate antimicrobial sensitivity testing is essential to guide effective treatment. Here, we present "simplified 5PSeq" (s5PSeq), a streamlined protocol...