Engineering PHL7 for improved poly(ethylene terephthalate) depolymerization via rational design and directed evolution [0.03%]
工程化改造PHL7蛋白以提高聚对苯二甲酸乙二醇酯的降解性能：理性设计和定向进化相结合的方法
Thomas M Groseclose,Erin Kober,Matilda Clark et al.
Thomas M Groseclose et al.
Enzymatic depolymerization of poly(ethylene terephthalate) (PET) has emerged as a promising approach for polyester recycling, and, to date, many natural and engineered PET hydrolase enzymes have been reported. For industrial use, PET hydrol...
SubTuner leverages physics-based modeling to complement AI in enzyme engineering toward non-native substrates [0.03%]
基于物理的建模技术助力人工智能进行酶工程改造以适应非天然底物
Qianzhen Shao,Asher C Hollenbeak,Yaoyukun Jiang et al.
Qianzhen Shao et al.
We developed SubTuner, a physics-based computational tool that tackles the challenge of identifying enzyme mutants with enhanced activity for specified non-native substrates. To test the performance of SubTuner, we designed three tasks - al...
Belt-Sulfur Mobilization as a Crucial Mechanistic Feature Shared between the Vanadium and Molybdenum Nitrogenases [0.03%]
钒氮酶和钼氮酶中共同存在的关键作用机制——带状硫的动员
Chi Chung Lee,Kuntal Chatterjee,Junko Yano et al.
Chi Chung Lee et al.
Nitrogenase catalyzes the reduction of N2 to NH3 at its active-site cofactor. Recent studies of the 'conventional' Mo-nitrogenase suggest a plausible involvement of all cofactor belt-S sites in catalysis. Here, we use analytical, enzymatic ...
Promoting CO2 reduction in the presence of oxygen with polymer-based gas diffusion electrodes [0.03%]
基于聚合物的气体扩散电极在氧气存在下促进二氧化碳还原
Sam Van Daele,Lieven Hintjens,Daniel Choukroun et al.
Sam Van Daele et al.
The electrochemical reduction of CO2 is a promising technology that holds the potential to convert waste CO2 into valuable products. High carbon capture and purification costs hamper economic feasibility and drive scientists to explore the ...
Ann Varghese,Sodiq O Waheed,Shobhit S Chaturvedi et al.
Ann Varghese et al.
The fat-mass and obesity-associated protein (FTO) is a Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenase of the AlkB family and is linked with obesity and cancer. The enzyme is identified as single-stranded DNA/RNA demethylase with N6-met...
Copper-Catalyzed 1,4-Aminohydroxylation and Aminothiolation of 1,3-Dienes by Carbonyl-Assisted Migration [0.03%]
羰基辅助迁移铜催化1,3-丁二烯的1,4-amination氧化和加硫反应
Noah H Watkins,Yungeun Kwon,Qiu Wang
Noah H Watkins
We report a copper-catalyzed 1,4-selective aminooxygenation of 1,3-dienes as a direct entry to 1,4-allylic amino alcohols. The reactions are effective on a diverse range of amide, urea, and ester-containing 1,3-dienes, allowing for facile i...
Enantioselective synthesis of axially chiral P-containing allenes for allenyl chirality [0.03%]
手性P原子轴不对称联二烯的对映选择性合成及其联二烯手性的应用研究
Guangshou Feng,Qiu Wang
Guangshou Feng
In this issue of Chem Catalysis, Wang J. and co-workers have established an efficient and practical route to construct versatile di-, tri-, and tetra-substituted phosphoryl allenes via a nickel-catalyzed propargylic substitution reaction, o...
Expanding the repertoire of imine reductases by mining divergent biosynthetic pathways for promiscuous reactivity [0.03%]
通过挖掘发散的生物合成途径来扩展亚胺还原酶的多样性反应库
Godwin A Aleku,Florian Hollfelder
Godwin A Aleku
Imine reductases (IREDs) are invaluable catalysts for enantioselective imine reduction and reductive amination of carbonyl compounds. Their synthetic versatility is, however, limited by their substrate scope, and new IREDs are needed. Curre...
Chiral pyrrolidines via an enantioselective Hofmann-Löffler-Freytag reaction [0.03%]
手性吡咯烷的霍夫曼-洛弗尔-弗莱塔反应法合成
Pavitra Laohapaisan,Ipshita Roy,David A Nagib
Pavitra Laohapaisan
Radical C-H aminations enable rapid access to the most common heterocycles in medicines (e.g. pyrrolidines), yet stereocontrol of these powerful transformations remains a challenge. Here, we report the discovery of the first enantio- and re...
Visible Light-Driven Excited-State Copper-BINAP Catalysis for Accessing Diverse Chemical Reactions [0.03%]
可见光驱动激发态铜-BINAP催化多样性化学反应
Upasana Mukherjee,Jagrut A Shah,Ming-Yu Ngai
Upasana Mukherjee
The use of visible light to drive chemical transformations has a history spanning over a century. However, the development of photo-redox catalysts to efficiently harness light energy is a more recent advancement, evolving over the past two...