Samitha S Somathilaka,Sasitharan Balasubramaniam,Daniel P Martins et al.
Samitha S Somathilaka et al.
Bacteria are known to interpret a range of external molecular signals that are crucial for sensing environmental conditions and adapting their behaviors accordingly. These external signals are processed through a multitude of signaling tran...
Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles [0.03%]
基于粒子的相位FLIM-FRET解析单个病毒颗粒内的蛋白质相互作用
Quinten Coucke,Nagma Parveen,Guillermo Solís Fernández et al.
Quinten Coucke et al.
Fluorescence lifetime imaging microscopy (FLIM) is a popular modality to create additional contrast in fluorescence images. By carefully analyzing pixel-based nanosecond lifetime patterns, FLIM allows studying complex molecular populations....
Thermodynamically consistent determination of free energies and rates in kinetic cycle models [0.03%]
动力学循环模型中自由能量和速率的热力学一致确定方法
Ian M Kenney,Oliver Beckstein
Ian M Kenney
Kinetic and thermodynamic models of biological systems are commonly used to connect microscopic features to system function in a bottom-up multiscale approach. The parameters of such models-free energy differences for equilibrium properties...
Cytosolic Ca2+ gradients and mitochondrial Ca2+ uptake in resting muscle fibers: A model analysis [0.03%]
休息肌肉纤维细胞质钙离子梯度与线粒体钙离子摄取的模型分析
Lorenzo Marcucci,Antonio Michelucci,Carlo Reggiani
Lorenzo Marcucci
Calcium ions (Ca2+) enter mitochondria via the mitochondrial Ca2+ uniporter, driven by electrical and concentration gradients. In this regard, transgenic mouse models, such as calsequestrin knockout (CSQ-KO) mice, with higher mitochondrial ...
Ivan Terterov,Daniel Nettels,Dmitrii E Makarov et al.
Ivan Terterov et al.
Quantifying biomolecular dynamics has become a major task of single-molecule fluorescence spectroscopy methods. In single-molecule Förster resonance energy transfer (smFRET), kinetic information is extracted from the stream of photons emit...
A transistor model for the cystic fibrosis transmembrane conductance regulator [0.03%]
囊性纤维化跨膜调节因子的晶体管模型
William D Hunt,Nael A McCarty,Eduardo Martinez Marin et al.
William D Hunt et al.
In this paper we present a transistor circuit model for cystic fibrosis transmembrane conductance regulator (CFTR) that seeks to map the functional form of CFTR both in wild type and mutants. The circuit architecture is configured so that t...
In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET [0.03%]
活体FRET小动物成像定量分析：基于强度与寿命的FRET对比研究
Jason T Smith,Nattawut Sinsuebphon,Alena Rudkouskaya et al.
Jason T Smith et al.
Förster resonance energy transfer (FRET) microscopy is used in numerous biophysical and biomedical applications to monitor inter- and intramolecular interactions and conformational changes in the 2-10 nm range. FRET is currently being exte...
Comparing confocal and two-photon Ca2+ imaging of thin low-scattering preparations [0.03%]
共焦和双光子钙成像的比较（用于薄低散射样品）
Jinbo Cheng,Shane M McMahon,David W Piston et al.
Jinbo Cheng et al.
Ca2+ imaging provides insight into biological processes ranging from subcellular dynamics to neural network activity. Two-photon microscopy has assumed a dominant role in Ca2+ imaging. The longer wavelength infra-red illumination undergoes ...
Macy Payne,Ivina Mali,Thomas Mueller et al.
Macy Payne et al.
Magnetic resonance imaging (MRI) is a highly significant imaging platform for a variety of medical and research applications. However, the low spatiotemporal resolution of conventional MRI limits its applicability toward rapid acquisition o...
MeltR software provides facile determination of nucleic acid thermodynamics [0.03%]
MeltR软件可方便地确定核酸热力学性质
Jacob P Sieg,Sebastian J Arteaga,Brent M Znosko et al.
Jacob P Sieg et al.
Thermodenaturation (melting) curves of macromolecules are used to determine folding thermodynamic parameters. Notably, this insight into RNA and DNA stability underlies nearest neighbor theory and diverse structure prediction tools. Analysi...