Phasor identifier: A cloud-based analysis of phasor-FLIM data on Python notebooks [0.03%]
相量标识符：基于Python软件包的相量-荧光寿命成像显微镜数据分析云计算系统
Mario Bernardi,Francesco Cardarelli
Mario Bernardi
This paper introduces an innovative approach utilizing Google Colaboratory for the versatile analysis of phasor fluorescence lifetime imaging microscopy (FLIM) data collected from various samples (e.g., cuvette, cells, tissues) and in vario...
Trustworthy in silico cell labeling via ensemble-based image translation [0.03%]
基于集成的图像转换中的可信的细胞合成标注方法
Sara Imboden,Xuanqing Liu,Marie C Payne et al.
Sara Imboden et al.
Artificial intelligence (AI) image translation has been a valuable tool for processing image data in biological and medical research. To apply such a tool in mission-critical applications, including drug screening, toxicity study, and clini...
Thioflavin T indicates mitochondrial membrane potential in mammalian cells [0.03%]
地塞米松诱导的哺乳动物细胞中线粒体膜电位指示剂的可能性
Emily Skates,Hadrien Delattre,Zoe Schofield et al.
Emily Skates et al.
The fluorescent benzothiazole dye thioflavin T (ThT) is widely used as a marker for protein aggregates, most commonly in the context of neurodegenerative disease research and diagnosis. Recently, this same dye was shown to indicate membrane...
Establishing Riboglow-FLIM to visualize noncoding RNAs inside live zebrafish embryos [0.03%]
建立Riboglow-FLIM以在活斑马鱼胚胎中可视化非编码RNA
Nadia Sarfraz,Harrison J Lee,Morgan K Rice et al.
Nadia Sarfraz et al.
The central role of RNAs in health and disease calls for robust tools to visualize RNAs in living systems through fluorescence microscopy. Live zebrafish embryos are a popular system to investigate multicellular complexity as disease models...
Model-based trajectory classification of anchored molecular motor-biopolymer interactions [0.03%]
基于模型的轨迹分类研究锚定分子马达-生物聚合物相互作用
John B Linehan,Gerald Alan Edwards,Vincent Boudreau et al.
John B Linehan et al.
During zygotic mitosis in many species, forces generated at the cell cortex are required for the separation and migration of paternally provided centrosomes, pronuclear migration, segregation of genetic material, and cell division. Furtherm...
Decoding stoichiometric protein synthesis in E. coli through translation rate parameters [0.03%]
通过翻译速率参数解码大肠杆菌中的计量学蛋白质合成
Inayat Ullah Irshad,Ajeet K Sharma
Inayat Ullah Irshad
E. coli is one of the most widely used organisms for understanding the principles of cellular and molecular genetics. However, we are yet to understand the origin of several experimental observations related to the regulation of gene expres...
Dag Chun Standnes
Dag Chun Standnes
Uptake of oxygen by hemoglobin (Hb), described by the oxygen-Hb dissociation curve, is obviously important for the existence of all vertebrates. Its sigmoidal curve shape indicates that oxygen binds more tightly if sites already are occupie...
Neural network-assisted single-molecule localization microscopy with a weak-affinity protein tag [0.03%]
基于弱亲和力蛋白质标签的神经网络辅助单分子定位显微镜技术
Soohyen Jang,Kaarjel K Narayanasamy,Johanna V Rahm et al.
Soohyen Jang et al.
Single-molecule localization microscopy achieves nanometer spatial resolution by localizing single fluorophores separated in space and time. A major challenge of single-molecule localization microscopy is the long acquisition time, leading ...
Real-time detection of virus antibody interaction by label-free common-path interferometry [0.03%]
基于共路径干涉的实时无标记病毒抗体检测方法研究
Samer Alhaddad,Houda Bey,Olivier Thouvenin et al.
Samer Alhaddad et al.
Viruses have a profound influence on all forms of life, motivating the development of rapid and minimally invasive methods for virus detection. In this study, we present a novel methodology that enables quantitative measurement of the inter...
Evolution of drug resistance drives destabilization of flap region dynamics in HIV-1 protease [0.03%]
耐药性进化驱动HIV-1蛋白酶翼区动力学不稳定性发展
Madhusudan Rajendran,Maureen C Ferran,Leora Mouli et al.
Madhusudan Rajendran et al.
The HIV-1 protease is one of several common key targets of combination drug therapies for human immunodeficiency virus infection and acquired immunodeficiency syndrome. During the progression of the disease, some individual patients acquire...