Raviv Dharan,Alisa Vaknin,Raya Sorkin
Raviv Dharan
Tetraspanin 4, a protein with four transmembrane helices and three connecting loops, senses membrane curvature and localizes to membrane tubes. This enrichment in tubular membranes enhances its diverse interactions. While the transmembrane ...
Diffusion kinetics and perfusion times in tissue models obtained by bioorthogonal Raman μ-spectroscopy [0.03%]
基于生物正交拉曼μ光谱的组织模型中的扩散动力学和灌注时间
Saskia Altmaier,Ina Meiser,Frank Stracke et al.
Saskia Altmaier et al.
The penetration kinetics of small-molecule compounds like nutrients, drugs, and cryoprotective agents into artificial cell aggregates are of pivotal relevance in many applications, from stem cell differentiation and drug screening through t...
Christopher Tyson,Santosh Gaire,Ian Pegg et al.
Christopher Tyson et al.
We present a method for tracking densely clustered, high-velocity, indistinguishable objects being spawned at a high rate and moving in a directed force field using only object centroids as inputs and no other image information. The algorit...
Philament: A filament tracking program to quickly and accurately analyze in vitro motility assays [0.03%]
Philament：一个快速准确分析体外动力学测定的追踪程序
Ryan M Bowser,Gerrie P Farman,Carol C Gregorio
Ryan M Bowser
In vitro motility (IVM) assays allow for the examination of the basic interaction between cytoskeletal filaments with molecular motors and the influence many physiological factors have on this interaction. Examples of factors that can be st...
Deciphering amyloid fibril molecular maturation through FLIM-phasor analysis of thioflavin T [0.03%]
通过噻氯氟霉素T的FLIM-phasor分析破译淀粉样纤维分子成熟过程
Sara Anselmo,Giuseppe Sancataldo,Valeria Vetri
Sara Anselmo
The investigation of amyloid fibril formation is paramount for advancing our understanding of neurodegenerative diseases and for exploring potential correlated therapeutic strategies. Moreover, the self-assembling properties of amyloid fibr...
Specific anchoring of large topologically closed DNA for single-molecule protein:DNA interactions [0.03%]
大拓扑闭合DNA的特定位点锚定用于单分子蛋白质:DNA相互作用的研究
Neville S Gilhooly,Stephen C Kowalczykowski
Neville S Gilhooly
Single-molecule and bulk biophysical approaches to study protein-DNA interactions on surface-immobilized nucleic acid templates typically rely on modifying the ends of linear DNA molecules to enable surface-DNA attachments. Unless both stra...
Quantifying the minimum localization uncertainty of image scanning localization microscopy [0.03%]
图像扫描定位显微镜的最小定位不确定性量化
Dylan Kalisvaart,Shih-Te Hung,Carlas S Smith
Dylan Kalisvaart
Modulation enhanced single-molecule localization microscopy (meSMLM), where emitters are sparsely activated with sequentially applied patterned illumination, increases the localization precision over single-molecule localization microscopy ...
Empirical asymmetric phonon sideband due to phonons in the protein matrix present in photosynthetic complexes: Time-domain response theory [0.03%]
存在于光合复合物中的蛋白质基质中声子引起的实测不对称声子边带：时间域响应理论
Mohamad Toutounji
Mohamad Toutounji
The phonon spectral density plays a key role in probing the dynamical and spectral behavior of molecular aggregates. One may utilize the intimate connection between the one-phonon profile and the phonon spectral density to extract a plausib...
Andrew Killeen,Thibault Bertrand,Chiu Fan Lee
Andrew Killeen
Active nematics is an emerging paradigm for characterizing biological systems. One aspect of particularly intense focus is the role active nematic defects play in these systems, as they have been found to mediate a growing number of biologi...
Mohammad Sharifian Gh,Michael J Wilhelm,Hai-Lung Dai
Mohammad Sharifian Gh
Mechanosensitive (MS) channels act to protect the cytoplasmic membrane (CM) of living cells from environmental changes in osmolarity. In this report, we demonstrate the use of time-resolved second-harmonic light scattering (SHS) as a means ...