Yeast complementation assays provide limited informationon functional features of K+ channels [0.03%]
酵母补救实验只能提供有限的关于钾通道的功能特征的信息
Kerri Kurkovetz,Matea Cartolano,Manuela Gebhardt et al.
Kerri Kurkovetz et al.
We investigate to what extent yeast complementation assays, which in principle can provide large amounts of training data for machine learning models, yield quantitative correlations between growth rescue and single channel recordings. If t...
Deep Learning-Driven Automated High-Content dSTORM Imaging with a scalable Open Source Toolkit [0.03%]
基于深度学习的可扩展开源工具包驱动的自动高内涵dSTORM成像
Janis T Linke,Luise Appeltshauser,Kathrin Doppler et al.
Janis T Linke et al.
Super-resolution microscopy offers the ability to visualize molecular structures in biological samples with unprecedented detail. However, the full potential of these techniques is often hindered by a lack of automated, user-independent wor...
Wessel S Rodenburg,Sven F A Ebben,Jorine M Eeftens
Wessel S Rodenburg
Mechanical properties of cells are closely related to function and play a crucial role in many cellular processes, including migration, differentiation, and cell fate determination. Numerous methods have been developed to assess cell mechan...
Marcel Streit,Made Budiarta,Marvin Jungblut et al.
Marcel Streit et al.
Super-resolution microscopy (SRM) has transformed biological imaging by circumventing the diffraction limit of light and enabling the visualization of cellular structures and processes at the molecular level. Central to the capabilities of ...
Overexpression, Biophysical and Functional Characterization of a Recombinant FGF21 (rFGF21) [0.03%]
重组FGF21(rFGF21)的过表达及其生物物理和功能表征
Phuc Phan,Jason Hoang,Thallapuranam Krishnaswamy Suresh Kumar
Phuc Phan
Fibroblast Growth Factor 21 (FGF21) is an endocrine FGF that plays a vital role in regulating essential metabolic pathways. FGF21 increases glucose uptake by cells, promotes fatty acid oxidation, reduces blood glucose levels, and alleviates...
Towards measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime [0.03%]
基于荧光寿命的芽孢杆菌膜电位的绝对测量
Debjit Roy,Xavier Michalet,Evan W Miller et al.
Debjit Roy et al.
Membrane potential (MP) changes can provide a simple readout of bacterial functional and metabolic state or stress levels. While several optical methods exist for measuring fast changes in MP in excitable cells, there is a dearth of such me...
Correlating Disordered Activation Domain Ensembles with Gene Expression Levels [0.03%]
无序激活结构域与基因表达水平的关系研究
Eduardo Flores,Aleah R Camacho,Estefania Cuevas-Zepeda et al.
Eduardo Flores et al.
Transcription factor proteins bind to specific DNA promoter sequences and initiate gene transcription. These proteins often contain intrinsically disordered activation domains (ADs) that regulate their transcriptional activity. Like other d...
DiffMAP-GP: Continuous 2D Diffusion Maps from Particle Trajectories without Data Binning using Gaussian Processes [0.03%]
基于高斯过程的连续二维扩散图谱算法_diffmap-gp：利用高斯过程从粒子轨迹中获得连续二维扩散图谱无需数据离散化处理
Vishesh Kumar,J Shepard Bryan th,Alex Rojewski et al.
Vishesh Kumar et al.
Diffusion coefficients often vary across regions, such as cellular membranes, and quantifying their variation can provide valuable insight into local membrane properties such as composition and stiffness. Toward quantifying diffusion coeffi...
CD spectra reveal the state of G-quadruplexes and i-motifs in repeated and other DNA sequences [0.03%]
圆二色谱揭示重复序列和其他DNA序列中G-四链体和i- motif的状态
Levi Diggins,Daniel Ross,Sundeep Bhanot et al.
Levi Diggins et al.
The B-DNA of the genome contains numerous sequences that can form various noncanonical structures including G-quadruplex (G4), formed by two or more stacks of four guanine residues in a plane, and intercalating motif (i-motif [iM]) formed b...
TC10 differently controls the dynamics of Exo70 in growth cones of cortical and hippocampal neurons [0.03%]
TC10调控皮层和海马神经元生长锥中Exo70的动态过程有所不同
Hiteshika Gosain,Karin B Busch
Hiteshika Gosain
The exocyst is an octameric protein complex that acts as a tether for GOLGI-derived vesicles at the plasma membrane during exocytosis. It is involved in membrane expansion during axonal outgrowth. Exo70 is a major subunit of the exocyst com...