Optimizing a Coarse-Grained Model for Large-Scale Membrane Protein Simulation [0.03%]
用于大规模膜蛋白模拟的粗粒化模型优化
Chen Yun Wen,Yun Lyna Luo,Jesper J Madsen
Chen Yun Wen
Coarse-grained (CG) models are widely used to study membrane proteins at physiologically relevant scales. However, simulating long-range bilayer deformations induced by membrane-embedded proteins at submicrometer scales remains challenging....
An Antarctic toothfish eye lens protein resists thermal stress even when extensively deamidated [0.03%]
脱氨处理后南极冰鱼眼睛晶状体蛋白仍能抵抗热应力
Collin Sroge,Vanessa Encinas,Zane G Long et al.
Collin Sroge et al.
Crystallins are highly stable, soluble proteins that refract light and maintain transparency in the vertebrate eye lens. They are not replaced after early development, making them an excellent system for studying protein stability and solub...
Refractive index as an indicator for dynamic protein condensation in cell nuclei [0.03%]
折射率作为细胞核中蛋白质凝聚的动力学指示器
Orlando Marin,Peter Kirchweger,Arina Dalaloyan et al.
Orlando Marin et al.
Protein condensation is the basis for formation of membrane-less organelles in the cell. Most famously, weak, polyvalent interactions, often including RNA, may lead to a liquid-liquid phase separation. This effect greatly enhances local con...
Sequence and supercoiling-dependent effects on the structural dynamics of DNA minicircles [0.03%]
序列和超螺旋对DNA迷你环结构动力学的影响
Manuel Micheloni,Luca Tubiana,Raffaello Potestio et al.
Manuel Micheloni et al.
The degree of over/under-winding of the DNA double helix quantified by the superhelical density, is a key feature modulating critical biological processes such as gene expression and regulation: In fact, DNA molecules are able to channel th...
Yonglei Sun,Quan Wang
Yonglei Sun
Measuring dipole orientations of fluorescent probes offers unique local structural insights of labelled biomolecules and has seen expanding applications in structural biology studies. Here we propose an alternative imaging geometry, 'dual-v...
Scanning electron microscopy of hyphal ectobiont bacteria within mycelial extracellular matrices [0.03%]
菌丝外生物细菌的扫描电子显微镜观察——胞外基质中的菌丝外生物细菌的扫描电镜观测
Davin Browner,Andrew Adamatzky
Davin Browner
Fungi and bacteria are found living in a wide variety of environments and their interactions are important in many processes including soil health, human and animal physiology and in biotechnological applications. Here, we investigate a sin...
Skeletal Muscle Alpha Actin (ACTA1) Acetylation Enhances Myosin Binding and Increases Calcium Sensitivity [0.03%]
乙酰化骨骼肌α-actin(ACTA1)增强与肌球蛋白结合和增加钙敏感性
Samantha S Romanick,Luis Godoy,Adrian Lopez et al.
Samantha S Romanick et al.
Skeletal muscle alpha actin (ACTA1) is important for muscle contraction and relaxation, with historical studies focused on ACTA1 mutations in muscle dysfunction. Proteomics reports have consistently observed that actin, including ACTA1, is ...
Escherichia coli FocA/B-dependent H+ and K+ fluxes: influence of exogenous versus endogenous formate [0.03%]
大肠杆菌FocA/B依赖型H+和K+通量：外源与内源甲酸的影响
L Grigoryan,A Babayan,A Vassilian et al.
L Grigoryan et al.
Escherichia coli translocates formate/formic acid bidirectionally across the cytoplasmic membrane by the FocA/FocB formate channels during fermentation. Depending on the pH and whether formate is supplied exogenously or generated internally...
Assessing Protein-Lipid Interactions with a Low-Cost, Accessible Centrifugation Assay [0.03%]
一种低成本的离心检测方法评估蛋白质与脂质的相互作用
Citlayi G Villaseñor,Alexandra Karagiaridi,Valentina S Dimitrova et al.
Citlayi G Villaseñor et al.
Assessing protein insertion and association with membranes is often a critical step that follows protein synthesis for both fundamental studies on protein folding and structure as well as translational applications that harness proteins for...
Single molecule localization microscopy error is sensor-dependent and larger than theory predicts [0.03%]
一种分子定位显微术的误差取决于传感器，并且比理论预测的大
Alfonso Brenlla-Lopez,Laila Deen,Paolo Annibale
Alfonso Brenlla-Lopez
Since the advent of stochastic localization microscopy approaches in 2006, the number of studies employing this strategy to investigate the sub-diffraction limit features of fluorescently labeled structures in biology, biophysics and solid ...