Recharging your fats: CHARMM36 parameters for neutral lipids triacylglycerol and diacylglycerol [0.03%]
中性脂质甘油三酯和甘油二酯的CHARMM36参数计算
Pablo Campomanes,Janak Prabhu,Valeria Zoni et al.
Pablo Campomanes et al.
Neutral lipids (NLs) are an abundant class of cellular lipids. They are characterized by the total lack of charged chemical groups in their structure, and, as a consequence, they play a major role in intracellular lipid storage. NLs that ca...
Optical probing of local membrane potential with fluorescent polystyrene beads [0.03%]
用荧光聚苯乙烯珠测量局部膜电位的光学方法
Zehavit Shapira,Nurit Degani-Katzav,Shimon Yudovich et al.
Zehavit Shapira et al.
The study of electrical activity in single cells and local circuits of excitable cells, such as neurons, requires an easy-to-use, high-throughput methodology that allows for the measurement of membrane potential. Investigating the electrica...
Ana C Chang-Gonzalez,Holly C Gibbs,Arne C Lekven et al.
Ana C Chang-Gonzalez et al.
We introduce a computational approach to build three-dimensional (3D) surface mesh models of the early-stage zebrafish brain primordia from time-series microscopy images. The complexity of the early-stage brain primordia and lack of recogni...
Cell-free electrophysiology of human VDACs incorporated into nanodiscs: An improved method [0.03%]
人类VDACs融入纳米盘的无细胞电生理学：一种改进的方法
Stefano Conti Nibali,Maria Carmela Di Rosa,Oliver Rauh et al.
Stefano Conti Nibali et al.
Voltage-dependent anion-selective channel (VDAC) is one of the main proteins of the outer mitochondrial membrane of all eukaryotes, where it forms aqueous, voltage-sensitive, and ion-selective channels. Its electrophysiological properties h...
Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs [0.03%]
单荧光纳米盘通过点定位、位点特异性膜电位光学记录
Asaf Grupi,Zehavit Shapira,Shimon Yudovich et al.
Asaf Grupi et al.
Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was de...
Microscopic Origin of the Spatial and Temporal Precision in Biological Systems [0.03%]
生物系统空间和时间精度的微观起源
All living systems display remarkable spatial and temporal precision, despite operating in intrinsically fluctuating environments. It is even more surprising given that biological phenomena are regulated by multiple chemical reactions that ...