A home for the genome [0.03%]
基因组也有一个家了
Laura M Zahn
Laura M Zahn
Manel Esteller
Manel Esteller
Mouse models are widely used in biomedical sciences and in epigenetic studies, yet a simple way to interrogate the mouse DNA methylation was lacking. In this issue of Cell Genomics, Zhou et al.1 describe a mouse DNA methylation microarray t...
David Ichikawa,Marcus Noyes
David Ichikawa
The discovery of regulatory domains has been limited to the investigation of transcription factors and homologous protein sequences. In this issue of Cell Genomics, motivated by an oncogenic protein fusion, Tak et al.1 direct the regulatory...
Katie Teng,Hartland Warren Jackson
Katie Teng
Tissue-tumor interactivity is the culmination of cell intrinsic features and their extrinsic interactions with the environment. Recently in Cell, Dhainaut and Rose et al. established a strategy to track pooled CRISPR-modified cells in vivo ...
Unscrambling cancer genomes via integrated analysis of structural variation and copy number [0.03%]
通过结构变异和拷贝数的综合分析解开癌症基因组
Charles Shale,Daniel L Cameron,Jonathan Baber et al.
Charles Shale et al.
Complex somatic genomic rearrangements and copy number alterations are hallmarks of nearly all cancers. We have developed an algorithm, LINX, to aid interpretation of structural variant and copy number data derived from short-read, whole-ge...
Open for connections: HiCAR reveals the interactions of accessible DNA [0.03%]
开放的连接：HiCAR揭示易接近DNA的互作关系
Benoit Moindrot,Daan Noordermeer
Benoit Moindrot
Hi-C is a powerful technology for exploring 3D genome organization on a genome-wide scale, yet it can be financially and computationally challenging. In a recent issue of Molecular Cell, Wei et al.1 introduce HiCAR, which simplifies Hi-C by...
Eva Gracia Villacampa,Ludvig Larsson,Reza Mirzazadeh et al.
Eva Gracia Villacampa et al.
Formalin-fixed paraffin embedding (FFPE) is the most widespread long-term tissue preservation approach. Here, we report a procedure to perform genome-wide spatial analysis of mRNA in FFPE-fixed tissue sections, using well-established, comme...
A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation [0.03%]
介导细胞类型特异性基因调控的DNA基元组合的顺式调控词典
Laura K H Donohue,Margaret G Guo,Yang Zhao et al.
Laura K H Donohue et al.
Gene expression is controlled by transcription factors (TFs) that bind cognate DNA motif sequences in cis-regulatory elements (CREs). The combinations of DNA motifs acting within homeostasis and disease, however, are unclear. Gene expressio...
Meta-analysis fine-mapping is often miscalibrated at single-variant resolution [0.03%]
元分析精细定位在单变异分辨率下经常校准错误
Masahiro Kanai,Roy Elzur,Wei Zhou;Global Biobank Meta-analysis Initiative;Mark J Daly et al.
Masahiro Kanai et al.
Meta-analysis is pervasively used to combine multiple genome-wide association studies (GWASs). Fine-mapping of meta-analysis studies is typically performed as in a single-cohort study. Here, we first demonstrate that heterogeneity (e.g., of...
Heather R Keys,Kristin A Knouse
Heather R Keys
A complete understanding of the genetic determinants underlying mammalian physiology and disease is limited by the capacity for high-throughput genetic dissection in the living organism. Genome-wide CRISPR screening is a powerful method for...