Deciphering proteins in Alzheimer's disease: A new Mendelian randomization method integrated with AlphaFold3 for 3D structure prediction [0.03%]
阿尔茨海默病蛋白质组学的孟德尔随机化研究：结合AlphaFold2.0预测蛋白三维结构的新方法
Minhao Yao,Gary W Miller,Badri N Vardarajan et al.
Minhao Yao et al.
Hidden confounding biases hinder identifying causal protein biomarkers for Alzheimer's disease in non-randomized studies. While Mendelian randomization (MR) can mitigate these biases using protein quantitative trait loci (pQTLs) as instrume...
Single-nucleus multi-omics analyses reveal cellular and molecular innovations in the anterior cingulate cortex during primate evolution [0.03%]
单核多组学分析揭示前扣带回皮质在灵长类动物进化中的细胞和分子创新
Jiamiao Yuan,Kangning Dong,Haixu Wu et al.
Jiamiao Yuan et al.
The anterior cingulate cortex (ACC) of the human brain is involved in higher-level cognitive functions such as emotion and self-awareness. We generated profiles of human and macaque ACC gene expression and chromatin accessibility at single-...
Cell type and dynamic state govern genetic regulation of gene expression in heterogeneous differentiating cultures [0.03%]
细胞类型和动态状态在异质分化培养物的基因表达遗传调控中的作用
Joshua M Popp,Katherine Rhodes,Radhika Jangi et al.
Joshua M Popp et al.
Identifying the molecular effects of human genetic variation across cellular contexts is crucial for understanding the mechanisms underlying disease-associated loci, yet many cell types and developmental stages remain underexplored. Here, w...
Genome-wide investigation of VNTR motif polymorphisms in 8,222 genomes: Implications for biological regulation and human traits [0.03%]
基于8,222个基因组的泛基因组VNTR动机多态性研究及其对生物调控和人类表型的影响
Sijia Zhang,Qiao Song,Peng Zhang et al.
Sijia Zhang et al.
Variable number tandem repeat (VNTR) is a pervasive and highly mutable genetic feature that varies in both length and repeat sequence. Despite the well-studied copy-number variants, the functional impacts of repeat motif polymorphisms remai...
Genome-wide chromosome architecture prediction reveals biophysical principles underlying gene structure [0.03%]
全基因组染色体构象预测揭示了基因结构背后的基本物理原理
Michael Chiang,Chris A Brackley,Catherine Naughton et al.
Michael Chiang et al.
Classical observations suggest a connection between 3D gene structure and function, but testing this hypothesis has been challenging due to technical limitations. To explore this, we developed epigenetic highly predictive heteromorphic poly...
Rui Fan,Chunmei Cui,Boming Kang et al.
Rui Fan et al.
N6-methyladenosine (m6A) is the most prevalent chemical modification in eukaryotic mRNAs and plays key roles in diverse cellular processes. Precise localization of m6A sites is thus critical for characterizing the functional roles of m6A in...
Lost in translation: Illuminating protein mislocalization through high-content screening microscopy [0.03%]
从高内涵筛选成像角度看蛋白错定位的影响
Lucy G Dornan,Jeremy C Simpson
Lucy G Dornan
Establishing the subcellular distribution of all proteins encoded by the human genome remains a key objective of life science research. This is particularly important in the context of proteins that, through genetic sequencing of patients, ...
Jayne Hehir-Kwa
Jayne Hehir-Kwa
Jayne Hehir-Kwa is based at the Princess Máxima Center for Pediatric Oncology in the Netherlands and is an associate group leader within the Kemmeren group and the Big Data Core. Her work is focused on genomic and transcriptomic sequencing...
Seed sequences mediate off-target activity in the CRISPR-interference system [0.03%]
种子序列介导CRISPR干扰系统的脱靶效应
Neha Rohatgi,Jean-Philippe Fortin,Ted Lau et al.
Neha Rohatgi et al.
The CRISPR interference (CRISPRi) system is a powerful tool for selectively and efficiently silencing genes in functional genomics research applications. However, its off-target activity has not been systematically investigated. Here, we ut...
Liujia Qian,Rui Sun,Ruedi Aebersold et al.
Liujia Qian et al.
The insufficient availability of comprehensive protein-level perturbation data is impeding the widespread adoption of systems biology. In this perspective, we introduce the rationale, essentiality, and practicality of perturbation proteomic...