Protocol for generation of PD modeling induced neurons and detection of α-synuclein forms [0.03%]
帕金森病模型诱导神经元的生成及α-突触核蛋白形式检测的操作规范
Francesco V Nardi,Gulimiheranmu Maisumu,You Zhou et al.
Francesco V Nardi et al.
Alpha-synuclein (α-Syn) is an important molecule in the pathogenesis of Parkinson's disease and Alzheimer's disease-related dementias such as Lewy body dementia, forming multiple pathological species. In vitro disease models, including hum...
Protocol to calculate and compare exact Shapley values for different kernels in support vector machine models using binary features [0.03%]
一种计算和支持向量机模型中二元特征的不同核的精确Shapley值的协议
Jannik P Roth,Jürgen Bajorath
Jannik P Roth
The Shapley value formalism from cooperative game theory was adapted to explain predictions of machine learning models. Here, we present a protocol to calculate and compare exact Shapley values for support vector machine models with commonl...
Protocol for Xenium spatial transcriptomics studies using fixed frozen mouse brain sections [0.03%]
使用固定冷冻小鼠脑切片进行Xenium空间转录组学研究的协议
Xiaokuang Ma,Peng Chen,Jing Wei et al.
Xiaokuang Ma et al.
Here, we present a protocol for Xenium spatial transcriptomics studies using fixed frozen mouse brain sections. We describe steps for intracardiac perfusion, cryosectioning, and floating section mounting of brain sections, which enable runs...
Protocol for unified metabolomics and proteomics analysis of formalin-fixed paraffin-embedded tissue [0.03%]
福尔马林固定石蜡包被组织的代谢组学和蛋白质组学统一分析方案
Abisola R Isaiah,Laneke Luies,Du Toit Loots et al.
Abisola R Isaiah et al.
The use of archival formalin-fixed paraffin-embedded (FFPE) tissue samples for biochemical analyses is problematic because of the formation of a Schiff base, leading to low protein and metabolite yields during analytical extractions. Here, ...
Protocol to discover actionable cancer vulnerabilities enabled by neomorph protein-protein interactions with the AVERON Notebook [0.03%]
利用AVERON笔记本发现neo-蛋白互作以发掘可操作的癌症弱点的协议
Hongyue Chen,Brian Revennaugh,Haian Fu et al.
Hongyue Chen et al.
While some tumor driver mutations inhibit existing protein-protein interactions (PPIs), others can create neomorph interactions (neoPPIs) not characteristic of the wild-type counterparts. Such tumor-specific neoPPIs may represent targets fo...
Protocol for developing Pseudomonas aeruginosa type III secretion system-neutralizing monoclonal antibodies from human B cells [0.03%]
人B细胞发育产生抗铜绿假单胞菌分泌系统单克隆抗体的协议
Alexandra Albus,Christoph Kreer,Florian Klein et al.
Alexandra Albus et al.
Monoclonal antibodies (mAbs) targeting bacterial virulence factors may represent promising therapeutics in the fight against severe bacterial infections. Here, we present an approach for developing human-derived antibodies targeting the typ...
Protocols for rapid identification of small-molecule metabolite ligands interacting with proteins [0.03%]
用于快速鉴定与蛋白质结合的小分子元配体的协议
Baoyu He,Rou Zhao,Bin Zhang et al.
Baoyu He et al.
Metabolite-protein interactions have not been systematically studied due to a lack of effective techniques. Here, we present a protocol for identifying small-molecule metabolite ligands interacting with proteins. We describe steps for mixin...
Protocol for quantifying murine cardiomyocyte cell division by single-cell suspension [0.03%]
小鼠心肌细胞胞质分裂定量单细胞悬液法
Samantha K Swift,Alexandra L Purdy,Michaela Patterson
Samantha K Swift
The cardiac regeneration and development fields lack low-barrier-to-entry techniques that distinguish cardiomyocyte division from alternative outcomes in vivo. Here, we present a protocol in rodents to determine if cardiomyocyte cell divisi...
Protocol to study electrophysiological properties of hPSC-derived 3D cardiac organoids using MEA and sharp electrode techniques [0.03%]
基于hPSC的3D心脏类器官电生理特性的研究方案（微点差分放大器和尖端电极技术）
Ravichandra Venkateshappa,Zehra Yildirim,Shane R Zhao et al.
Ravichandra Venkateshappa et al.
Continuing advancements in human pluripotent stem cell (hPSC)-derived complex three-dimensional (3D) cardiac tissues require the development of novel technologies or adaptation of existing technologies to understand the physiology of the de...
Protocol for isolating specific C. elegans neuron types for bulk and single-cell RNA sequencing [0.03%]
用于批量和单细胞RNA测序的特定C. elegans神经元类型的分离方案
Seth R Taylor,Rebecca D McWhirter,Brittany K Matlock et al.
Seth R Taylor et al.
The C. elegans nervous system is compact and well described, ideal for identifying genetic programs that drive neuron-specific development, function, and connectivity. Here, we present a protocol for isolating specific neuron types for gene...