Protocol to purify human and mouse cytokines using an endotoxin-free Escherichia coli-based platform [0.03%]
一种使用无内毒素大肠杆菌平台纯化人和小鼠细胞因子的方法
Jae-Yoon Kim,Jessica Xin,I-Chih Kuo et al.
Jae-Yoon Kim et al.
Here, we present a cost-effective protocol to purify four representative human and mouse cytokines using an endotoxin-free Escherichia coli (E. coli)-based platform. We describe steps for protein expression and maltose-binding protein (MBP)...
Protocol for profiling circulating T cell subsets, their homing and regulatory markers, in pediatric samples using a 4-laser BD LSRII [0.03%]
用于儿科样本中循环T细胞亚群及其归巢和调节标记物的检测的四激光BD LSRIIProfiler实验流程
Tomas Thon,Jan Svoboda,Zaneta Slavickova et al.
Tomas Thon et al.
Here, we present a protocol for profiling T cell subsets, regulatory phenotypes, and gut- and skin-homing markers of unstimulated cells or those stimulated with staphylococcal enterotoxin B (SEB), lipopolysaccharide (LPS), or zymosan (ZYM)....
A step-by-step guide for isolating soluble and insoluble chromatin followed by next-generation sequencing library preparation [0.03%]
一步一步地进行可溶性和不可溶性染色质的分离，然后进行下一代测序文库构建指南
Shan Hua,Miraz A Sadi,Brandon J Park et al.
Shan Hua et al.
Mapping epigenetic features is essential for investigating chromatin regulation and gene expression control. We recently found that crosslinking- and sonication-based techniques preferentially exclude insoluble chromatin, restricting the in...
Immunofluorescence-based visualization of Malpighian tubule morphogenesis in Drosophila embryos [0.03%]
基于免疫荧光的果蝇胚胎Malpighi管形态发生成像
Neha Tiwari,Madhu G Tapadia
Neha Tiwari
Malpighian tubules (MTs) undergo extensive morphogenetic remodeling during Drosophila melanogaster embryogenesis, making them sensitive indicators of developmental perturbations. Here, we present a protocol to analyze embryonic MT developme...
Lyla El-Fayomi,Michael Bergamini,Derek van der Kooy
Lyla El-Fayomi
Neural implants are critical for modern mouse studies; however, permanent fixation of fibre optics or electrodes can render brain removal for histology particularly challenging. Frequent complaints include implant damage and tissue distorti...
Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing [0.03%]
使用集成单核RNA测序对小鼠肝脏和内脏脂肪组织进行最小偏差谱型分析的协议
Lei Li,Jonathan G Pol,Christophe Desterke et al.
Lei Li et al.
Single-nucleus RNA sequencing (snRNA-seq) enables transcriptomic profiling of tissues that are difficult to dissociate. Here, we present an integrated protocol combining tissue-specific nuclei extraction and selective enrichment to isolate ...
Protocol for generating multiplexed prime-edited monoclonal cell lines in porcine fetal fibroblasts [0.03%]
基于猪胚胎成纤维细胞的多靶点多编辑克隆系制备方案
Wenxin Duan,Yuyun Xing,Weiwei Liu
Wenxin Duan
Porcine fetal fibroblasts (PFFs) serve as standard donor cells for generating cloned pigs, and prime editing (PE) enables precise genome modification. Here, we describe a protocol for generating multiplexed prime-edited monoclonal cell line...
Protocol for cosmic-ray muon detection using plastic scintillators and photomultiplier tubes [0.03%]
用于宇宙射线缪子探测的塑料闪烁体和光电倍增管协议
Hamid Basiri,Jun Matsushima,Eduardo Cortina Gil et al.
Hamid Basiri et al.
Here, we present a protocol for cosmic-ray muon detection using plastic scintillators and photomultiplier tubes (PMT). We describe steps for assembling a scintillator-PMT detector, high-voltage plateauing and per-channel gain matching, and ...
Protocol for isolation of live tumor-infiltrating immune cells from immunocompetent murine brains for high-dimensional profiling [0.03%]
从免疫功能正常的小鼠脑中分离活的肿瘤浸润性免疫细胞进行高维谱型分析的协议
Aimée du Chatinier,Leyla Rigamonti,Piotr Waranecki et al.
Aimée du Chatinier et al.
Here, we present an optimized workflow to isolate viable immune cells from tumor-bearing mouse brains, with particular emphasis on myeloid populations, to enable detailed functional analysis of the tumor-immune microenvironment. We describe...
Protocol for generating and culturing high-grade serous ovarian carcinoma organoids from fresh or cryopreserved patient samples [0.03%]
来源于新鲜或冷冻保存的患者样本的高度浆液性卵巢癌类器官的制备和培养协议
Laura Gall-Mas,Lidia Moyano-Galceran,Elina Valkonen et al.
Laura Gall-Mas et al.
Stable long-term high-grade serous ovarian carcinoma (HGSC) organoids can be difficult to develop due to limited primary sample growth in 3D conditions. Here, we present a detailed protocol for the development of stable HGSC organoids, incl...