Protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes [0.03%]
成年果蝇卵巢细胞线粒体动态活细胞成像方案
Ankur Kumar,Hua Bai
Ankur Kumar
Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosoph...
Protocol for isolation of total RNA from mouse whole cochlea using organic solvents [0.03%]
有机溶剂法提取小鼠完整耳蜗总RNA的实验方法学探讨
Ezequiel Rías,Guillermo Spitzmaul,Leonardo Dionisio
Ezequiel Rías
Determining gene expression changes in cochlear components is important for understanding the mechanisms underlying hearing loss. Here, we present a protocol for the isolation of total RNA from mouse cochleae across a wide range of ages (2-...
Spatiotemporal analysis of cell division during symbiotic root nodule development in the model legume Medicago truncatula [0.03%]
模式豆类牧草 Médicago truncatula 根瘤发育共生过程中细胞分裂的时空分析
Georgina Wickens,Ella Greensmith,Katharina Schiessl
Georgina Wickens
This protocol combines rhizobial spot inoculation with deep-tissue imaging to capture cellular processes during early nodule development in the legume Medicago truncatula. We describe steps to visualize DNA replication activity and cell geo...
Protocol to perform cell-type-specific transcriptome-wide association study using scPrediXcan framework [0.03%]
使用scPrediXcan框架执行细胞类型特异性转录组范围关联研究的协议
Yichao Zhou,Sarah Sumner,Temidayo Adeluwa et al.
Yichao Zhou et al.
The scPrediXcan framework enables cell-type-specific transcriptome-wide association studies (TWASs) by integrating deep learning-based prediction of gene expression from DNA sequence and epigenetic features. We present a protocol for scPred...
Protocol for in vivo analysis of muscle function in porcine models for muscular dystrophies [0.03%]
用于肌肉营养不良模型猪的体内分析肌力的方案
Hristiyan Hristov,Michaela Blasi,Igor Neves Barbosa et al.
Hristiyan Hristov et al.
Porcine models carrying DMD mutations recapitulate Duchenne and Becker muscular dystrophy (DMD and BMD), inherited disorders leading to progressive muscle weakness, with DMD presenting a far more severe and rapidly progressive phenotype. Ac...
Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloids [0.03%]
人肾上皮细胞球状体的慢病毒工程和多组学表征方案
Giulia Perticari,Maroussia M P Ganpat,Jarno Drost
Giulia Perticari
Organoids derived from normal human tissue have proven to be effective preclinical models for studying tumor progression through the introduction of driver mutations. In this protocol, we outline techniques for disease modeling using organo...
Protocol for quantifying interaction patterns among genomic alterations in cancer [0.03%]
一种量化癌症中基因组改变之间相互作用模式的方法学
Adrián Maqueda-Real,Seokjin Ham,Laia Ollé-Monràs et al.
Adrián Maqueda-Real et al.
Publicly available large-scale cancer datasets allow for the systematic analysis of complex genomic alteration associations in tumorigenesis. Here, we present a protocol to quantify diverse interaction patterns between genomic alterations. ...
Protocol to enhance pre-sexual and sexual differentiation of Toxoplasma gondii using retinal cells and intestinal organoid-derived monolayers [0.03%]
利用视网膜细胞和肠类器官单层促进隐孢子虫性前分化和有性分化的方案
Saira Cancela,Florencia Sena,Romina Pagotto et al.
Saira Cancela et al.
Toxoplasma gondii undergoes pre-sexual and sexual differentiation primarily in feline hosts, limiting experimental study. Here, we present a protocol for enhancing T. gondii stage differentiation using non-feline in vitro systems. We descri...
Protocol for intranuclear Nrf2 detection in activated mouse, human, and Jurkat T cells by spectral flow cytometry [0.03%]
小鼠、人和Jurkat T细胞活化后胞核Nrf2检测的光谱流式细胞术方案
Aprajita Tripathi,Debolina Dasgupta,Mehak Ahuja et al.
Aprajita Tripathi et al.
Nuclear factor erythroid 2-related factor 2 (Nrf2) coordinates cellular antioxidation responses. Its expression increases in T cells upon activation. Here, we present a protocol for quantitative detection of intranuclear Nrf2 in activated m...
Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays [0.03%]
一种基于邻近连接的定量检测病毒蛋白和宿主蛋白相互作用的方法协议
Helene Hoenigsperger,Susanne Klute,Zoé Engels et al.
Helene Hoenigsperger et al.
The spatial organization of viral and cellular proteins shapes signal transduction. Here, we present a protocol to quantify differential protein-protein interactions by measuring their spatial association using proximity ligation assays (PL...