Deep phenotyping ILCs and T cells: A comparative analysis protocol for conventional and spectral flow cytometry [0.03%]
深入表型分析ILCs和T细胞：传统流式细胞术与光谱流式细胞术的比较分析方案
Jadie Acklam,Sukhveer K Mann,Karen G Hogg et al.
Jadie Acklam et al.
Here, we present a protocol for identifying human innate lymphoid cells (ILCs) and T cells using a 19-plex flow cytometry panel. We describe steps for titrating antibodies, preparing single-stain controls and samples, and staining cells for...
Protocol for establishing rectal tumor organoid-derived monolayers in a microphysiological system to model radiotherapy [0.03%]
基于直肠肿瘤类器官的单层细胞模型构建微流体芯片放射治疗研究体系的标准流程
Eloïse Bouges,Charlotte Segers,Marco Lezzerini et al.
Eloïse Bouges et al.
Patient-derived organoids offer greater human translational relevance than mouse models or immortalized cell lines, owing to their multicellular composition and patient-specific responses. Here, we present a protocol for establishing a huma...
Protocol for reconstructing 3D human skin equivalents on endogenous dermal matrix with optional pigmentation [0.03%]
在内源性真皮基质上重建具有可选色素的三维人体皮肤等效物的协议
Khek-Chian Tham,Zhi Heng Lau,Joycelyn X Y Lee et al.
Khek-Chian Tham et al.
Previous in vitro 3D skin models are limited by their reliance on animal-derived collagen, a poor representation of the human dermis, and low throughput. Here, we present a protocol for reconstructing 3D human skin equivalents on an endogen...
Protocol for deep phenotyping of human antigen-specific T cells across conventional and unconventional subsets by 22-color spectral flow cytometry [0.03%]
采用22色谱流式细胞仪对常规和非常规亚群人抗原特异性T细胞进行深度表型分析的方案
Jiahui Du,Mingzhe Zeng,Ziqian He et al.
Jiahui Du et al.
Analyzing antigen-specific T cells at the subset level is crucial for studying antiviral immunity and vaccine efficacy. We present a 22-color spectral flow cytometry protocol to characterize these cells from human PBMCs, covering convention...
Protocol for simultaneous non-invasive electrocardiogram and respiratory plethysmography recordings in mice [0.03%]
小鼠心电图和呼吸容积描记法的同步无创记录协议
Nicholas Y Iwakoshi,Stephen Salloum,Kevin J Cummings et al.
Nicholas Y Iwakoshi et al.
Here, we present a protocol for non-invasive simultaneous recording of electrocardiogram (ECG) and breathing in conscious mice. We describe steps for securing surface ECG electrodes with kinesiology tape, lightly sedating mice for lead plac...
Protocol for the generation of human medullary spinal trigeminal nucleus-like organoids and trigeminothalamic assembloids [0.03%]
人类脊髓三叉神经核样器官和三叉丘脑复合器官的生成方案
Wei Pang,Jinkui Zhu,Siyuan Chu et al.
Wei Pang et al.
Nucleus-specific brain organoids enable precise human brain research, yet refined hindbrain models are rare. Here, we present a guided differentiation protocol for generating human medullary spinal trigeminal nucleus-like organoids (hmSpVOs...
Protocol for the isolation of inducible GαsR201C explant cultures for ex vivo modeling of fibrous dysplasia of bone and pathologic osteoclastogenesis [0.03%]
用于骨纤维异常增殖症和病理性破骨细胞生成的体外模型的诱导型GαsR201C出芽培养分离方案
Morteza Abyadeh,Jarred M Whitlock
Morteza Abyadeh
Fibrous dysplasia (FD) is caused by activating mutations in GNAS that disrupt skeletal progenitor differentiation and promote osteoclast-mediated bone resorption. Here, we describe a protocol to isolate bone marrow stromal cells from doxycy...
Protocol for genotyping cephalopod sex using a skin swab and quantitative PCR [0.03%]
基于皮肤拭子和定量PCR进行头足类动物性别鉴定的方案
Frederick A Rubino,Connor J Gibbons,Thomas J Mungioli et al.
Frederick A Rubino et al.
The coleoid cephalopods (octopus, cuttlefish, and squid) are emerging model organisms for neuroscience, development, and evolutionary biology. Determining their sex early in life is critical for population management and controlled experime...
Protocol for evaluating microglial phagocytosis in situ in mouse hippocampal slices [0.03%]
小胶质细胞吞噬作用的原位评价方案——基于小鼠海马切片
Summer G Paulson,Fritz W Lischka,Jeremy D Rotty
Summer G Paulson
Microglia are the primary phagocytes of the central nervous system (CNS). Here, we present a protocol for microinjecting opsonized particles into mouse hippocampal slices followed by two-photon time-lapse microscopy. We describe steps for p...
Protocol for assessing enzymatic and non-enzymatic hydrogen sulfide production and levels [0.03%]
硫化氢产生和水平的酶促和非酶促测定规程
María Ángeles Cáliz-Molina,Inmaculada Pino-Pérez,András K Ponti et al.
María Ángeles Cáliz-Molina et al.
Hydrogen sulfide (H2S) is a key molecule in metabolism with important physiological effects. Despite its merits, detection and/or accurate determination remain challenging. Here, we present a protocol to determine H2S levels and cumulative ...