Protocol for specific induction of apoptosis or necroptosis in established murine tumors [0.03%]
小鼠移植瘤的凋亡和坏死程序诱导方案
Kay Hänggi,Brian Ruffell
Kay Hänggi
We present a protocol to study cancer cell death in vivo using mouse tumor models by generating cell lines that allow for the induction of "pure" apoptosis or necroptosis via an inducible dimerizer system. We describe steps for lentiviral t...
Titaya Lerskiatiphanich,Cedric S Cui,John D Lee
Titaya Lerskiatiphanich
Metabolic disturbances are common in motor neuron disease (MND), and elucidating their mechanisms may reveal therapies. Here, we present a protocol to assess glucose homeostasis in mice, including glucose tolerance, insulin tolerance, and g...
Protocol for the detection of RHIM-protein oligomeric complex formation using DSP-mediated crosslinking in HEK293T cultured cells [0.03%]
基于DSP交联的HEK293T细胞培养体系中RHIM蛋白寡聚复合物检测方案
Sanchita Mishra,Ayushi Amin Dey,Sannula Kesavardhana
Sanchita Mishra
Proteins harboring the RIP homotypic interaction motifs (RHIMs) can assemble into higher-order structures through RHIM-RHIM interactions. Here, we present a protocol for detecting RHIM protein oligomerization, which is central to cell death...
Protocol for immunodetection of α-synuclein pathology in paraffin-embedded liver tissues from murine models of Parkinson's disease [0.03%]
小鼠帕金森病模型石蜡包埋肝组织α突触核蛋白病理免疫检测方案
Martin Hallbeck,Maria Ntzouni,Martin Ingelsson et al.
Martin Hallbeck et al.
The accumulation of α-synuclein (α-Syn) pathology in peripheral tissues of Parkinson's disease (PD) has attracted growing scientific interest in recent years. Here, we present a protocol for the immunodetection of α-Syn pathology in muri...
Protocol for 3D Matrigel embedding of primary murine astrocytes for a physiologically relevant culture model [0.03%]
小鼠原代星形胶质细胞的三维基质胶培养体系构建及生理相关性分析protocols
Gurkirat Kaur,Maria Ester Salvucci,Veronica Giusti et al.
Gurkirat Kaur et al.
Primary astrocyte 2D cultures often exhibit a phenotype that differs significantly from astrocytes in the brain. Here, we present a detailed protocol for culturing primary murine astrocytes within a 3D Matrigel environment, which better mim...
Protocol to rapidly verify silent gene reporter in human pluripotent stem cells using CRISPR activation [0.03%]
使用CRISPR激活在人多能干细胞中快速验证沉默型基因报告者的方法协议
Youjun Wu,Aaron Zhong,Bernny Ramirez et al.
Youjun Wu et al.
Validating human pluripotent stem cell (hPSC) reporters targeting silent genes typically requires inducing gene expression through cell state transitions, which can be time consuming and complex. Here, we present a rapid workflow to verify ...
Protocol for mapping murine transcription factor interactomes and composite motifs combining affinity purification mass spectrometry and ChIP-seq [0.03%]
结合亲和纯化质谱及ChIP-seq的绘制小鼠转录因子相互作用组及复合基序的方案
Anna Gabele,Mert Cihan,Maximilian Sprang et al.
Anna Gabele et al.
Mass spectrometry (MS)-based approaches have significantly advanced our ability to study protein interaction networks in an unbiased manner. Here, we present a protocol that uses affinity purification (AP)-MS to identify interaction partner...
u-LINNDA: A protocol for user-optimized lymphoma identification through neural network detection aid [0.03%]
u-LINNDA：一种通过神经网络检测辅助进行用户优化的淋巴瘤识别协议
Maximilian Fischer,Miriam Cindy Maurer,Robin Peretzke et al.
Maximilian Fischer et al.
Glioblastoma (GBM) and primary central nervous system lymphoma (PCNSL) are aggressive brain tumors that require neurosurgical treatment. For targeted treatment, biopsy and histopathological identification of the tumor entity are necessary. ...
Protocol to quantify protein phosphorylation in mouse extended pluripotent stem cell-derived blastoids [0.03%]
小鼠扩展多能干细胞源性类囊胚蛋白质磷酸化定量方案
Tianxia Xiao,Yuxin Luo,Yang Yu
Tianxia Xiao
Protein phosphorylation is an important post-transcription modification regulating essential cellular activities. Here, we present a proteomics protocol to quantify protein phosphorylation in the extended pluripotent stem cell (EPSC)-derive...
Protocol to study metastatic dormancy using 4T07-mCherry breast cancer cells and an algorithm-based quantification of disseminated cancer cells [0.03%]
采用4T07-mCherry乳腺癌细胞和算法量化播散性癌细胞研究转移休眠的方案
Paulo Pereira,Virginia Cecconi,Maries van den Broek
Paulo Pereira
Dormant disseminated cancer cells are responsible for late relapses of breast cancer. Here, we present a protocol to orthotopically inject 4T07-mCherry breast cancer cells into mice and describe how to resect the primary tumor. We detail st...