Protocol for blastomere injection to generate bilateral hemimosaic Xenopus tadpoles [0.03%]
用于生成双侧半镶嵌斑马鱼蝌蚪的细胞注射方案
Vanessa J Li,Philip M Kesner,Anne Schohl et al.
Vanessa J Li et al.
Here, we present a protocol to generate bilateral hemimosaic Xenopus tadpoles that allows systematic, independent manipulation of pre- and postsynaptic neurons in the tadpole retinotectal projection. We describe the steps to perform in vitr...
Protocol for evaluating the economic viability of adiabatic compressed air energy storage systems [0.03%]
绝热压缩空气储能系统的经济性评估方法协议书
Danlei Yang,Yang Wang,Jihong Wang et al.
Danlei Yang et al.
Techno-economic evaluation is vital for assessing long-duration energy storage. Here, we present a computational workflow evaluating adiabatic compressed air energy storage economic performance. We describe steps for deriving learning-drive...
Protocol for modeling innate immune training by repeated alum exposure in mice (adjuvant conditioning) [0.03%]
铝佐剂反复刺激小鼠固有免疫记忆模型构建的方案（佐剂预处理）
Thais Boccia,Matheus Deroco Veloso da Silva,Victor Fattori et al.
Thais Boccia et al.
We present a protocol to model the effects of innate immune training and reprogramming through repeated alum exposure in mice, enabling the evaluation of immunoregulatory cell populations in both steady-state and inflammatory conditions. We...
Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs [0.03%]
人外周血单个核细胞光谱流式细胞术数据分析协议促进无监督聚类以探究人体衰老问题
Claudia J Krause,Akihide Higuchi,Shinji Tashiro et al.
Claudia J Krause et al.
Spectral flow cytometry enables the use of high-parameter panels. Using a Sony ID7000-optimized 45-color panel, we present a protocol for acquiring and preprocessing high-quality, high-parameter spectral flow cytometry data. We describe ste...
Yunyi Chen,Le Xu,Yuanyuan Yu et al.
Yunyi Chen et al.
Plant-derived extracellular vesicles (PEVs) are nanosized, lipid bilayer-enclosed vesicles secreted by plant cells into the extracellular space, playing critical roles in intercellular communication. Here, we present a comprehensive and rep...
Protocol to establish glioma stem cell spheroids and an enriched cancer-associated fibroblast population from a single patient tumor specimen [0.03%]
利用单一肿瘤样本建立胶质瘤干细胞球和富集的癌症相关成纤维细胞人群的协议
Caroline Delmas,Elisabeth Cohen-Jonathan-Moyal,Catherine Seva
Caroline Delmas
Glioblastoma is an aggressive, therapy-resistant brain tumor in which the role of cancer-associated fibroblasts (CAFs) remains poorly defined. We present a protocol to simultaneously establish glioblastoma stem cell (GSCs) spheroids and CAF...
Protocol for Nanodisc single-molecule pull-down assay to detect protein-lipid interactions [0.03%]
用于检测蛋白质-脂质相互作用的纳米盘单分子沉淀测定法标准操作规程
Adriana Reyes-Ordoñez,Shweta Shree,Stephen G Sligar et al.
Adriana Reyes-Ordoñez et al.
Phospholipids function as signaling molecules regulating numerous processes within the cellular environment. Identifying and characterizing the protein players involved in lipid-mediated functions remains a central focus. Here, we present a...
Protocol for quantitative analysis of adult zebrafish swimming behavior using DeepLabCut [0.03%]
使用DeepLabCut进行定量分析成年斑马鱼游泳行为的协议
Pramuk Keerthisinghe,Anwarul Karim,Aditya Basak et al.
Pramuk Keerthisinghe et al.
Here, we present a protocol for quantifying adult zebrafish swimming behavior using open-source software. We describe steps for recording and preprocessing swimming behavior videos, labeling and training a DeepLabCut network, and applying t...
Protocol for microinjection of rapamycin into the zebrafish habenula [0.03%]
雷帕霉素斑马鱼 Habenula 微注射实验方案
Olga Doszyn,Tomasz Dulski,Justyna Zmorzynska
Olga Doszyn
Published Erratum
STAR protocols. 2026 Feb 16;7(1):104407. DOI:10.1016/j.xpro.2026.104407 2026
Protocol for establishing a mouse model of bilateral maxillary first molar extraction to study alveolar bone healing [0.03%]
双侧第一磨牙拔除的小鼠模型建立方案以研究牙槽骨愈合问题
Jiarui Jiang,Jiayu Mou,Siwei Wang et al.
Jiarui Jiang et al.
Alveolar bone healing following tooth extraction is a complex biological process. Here, we present a protocol for establishing a reproducible mouse model of bilateral maxillary first molar extraction to study alveolar bone healing. We descr...